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miRNAs regulate acute transcriptional changes in broiler embryos in response to modification of incubation temperature

MicroRNAs are post-transcriptional regulators that play critical roles in diverse biological processes. We hypothesize that miRNAs may be involved in regulating transcriptome responses to changes in embryonic incubation temperature in chickens affecting differentiation and proliferation processes du...

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Autores principales: Naraballobh, Watcharapong, Trakooljul, Nares, Murani, Eduard, Krischek, Carsten, Janisch, Sabine, Wicke, Michael, Ponsuksili, Siriluck, Wimmers, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063901/
https://www.ncbi.nlm.nih.gov/pubmed/30054505
http://dx.doi.org/10.1038/s41598-018-29316-7
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author Naraballobh, Watcharapong
Trakooljul, Nares
Murani, Eduard
Krischek, Carsten
Janisch, Sabine
Wicke, Michael
Ponsuksili, Siriluck
Wimmers, Klaus
author_facet Naraballobh, Watcharapong
Trakooljul, Nares
Murani, Eduard
Krischek, Carsten
Janisch, Sabine
Wicke, Michael
Ponsuksili, Siriluck
Wimmers, Klaus
author_sort Naraballobh, Watcharapong
collection PubMed
description MicroRNAs are post-transcriptional regulators that play critical roles in diverse biological processes. We hypothesize that miRNAs may be involved in regulating transcriptome responses to changes in embryonic incubation temperature in chickens affecting differentiation and proliferation processes during tissue development. Therefore, we conducted comparative transcriptome profiling of miRNAs to examine altered expression in breast and hind muscle of embryos and day 35 chickens experiencing high (38.8 °C), control (37.8 °C), or low (36.8 °C) embryonic incubation temperature during embryonic day (ED) 7–10 or ED10–13. The results revealed differential expression of miRNAs due to modification of embryonic incubation temperature in a muscle type-specific and a developmental stage-specific manner. The immediate effects of thermal change observed in embryos were substantial compared to the subtle long-term effects in chickens at day 35 post-hatch. Upregulation of miR-133 in breast muscle and downregulation of miR-199a-5p, miR-1915, and miR-638 in hind muscle post ED7–10 high-temperature treatment are functionally associated with myogenesis and body size. ED10–13 low-temperature treatment led to downregulation of let-7, miR-93, and miR-130c that are related to proliferation and differentiation. The results provide insight into the dynamics of miRNA expression at variable embryonic incubation temperatures during developmental processes and indicate a major regulatory role of miRNAs in acute responses to modified environmental conditions that affect remodelling of cells and tissues.
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spelling pubmed-60639012018-07-31 miRNAs regulate acute transcriptional changes in broiler embryos in response to modification of incubation temperature Naraballobh, Watcharapong Trakooljul, Nares Murani, Eduard Krischek, Carsten Janisch, Sabine Wicke, Michael Ponsuksili, Siriluck Wimmers, Klaus Sci Rep Article MicroRNAs are post-transcriptional regulators that play critical roles in diverse biological processes. We hypothesize that miRNAs may be involved in regulating transcriptome responses to changes in embryonic incubation temperature in chickens affecting differentiation and proliferation processes during tissue development. Therefore, we conducted comparative transcriptome profiling of miRNAs to examine altered expression in breast and hind muscle of embryos and day 35 chickens experiencing high (38.8 °C), control (37.8 °C), or low (36.8 °C) embryonic incubation temperature during embryonic day (ED) 7–10 or ED10–13. The results revealed differential expression of miRNAs due to modification of embryonic incubation temperature in a muscle type-specific and a developmental stage-specific manner. The immediate effects of thermal change observed in embryos were substantial compared to the subtle long-term effects in chickens at day 35 post-hatch. Upregulation of miR-133 in breast muscle and downregulation of miR-199a-5p, miR-1915, and miR-638 in hind muscle post ED7–10 high-temperature treatment are functionally associated with myogenesis and body size. ED10–13 low-temperature treatment led to downregulation of let-7, miR-93, and miR-130c that are related to proliferation and differentiation. The results provide insight into the dynamics of miRNA expression at variable embryonic incubation temperatures during developmental processes and indicate a major regulatory role of miRNAs in acute responses to modified environmental conditions that affect remodelling of cells and tissues. Nature Publishing Group UK 2018-07-27 /pmc/articles/PMC6063901/ /pubmed/30054505 http://dx.doi.org/10.1038/s41598-018-29316-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Naraballobh, Watcharapong
Trakooljul, Nares
Murani, Eduard
Krischek, Carsten
Janisch, Sabine
Wicke, Michael
Ponsuksili, Siriluck
Wimmers, Klaus
miRNAs regulate acute transcriptional changes in broiler embryos in response to modification of incubation temperature
title miRNAs regulate acute transcriptional changes in broiler embryos in response to modification of incubation temperature
title_full miRNAs regulate acute transcriptional changes in broiler embryos in response to modification of incubation temperature
title_fullStr miRNAs regulate acute transcriptional changes in broiler embryos in response to modification of incubation temperature
title_full_unstemmed miRNAs regulate acute transcriptional changes in broiler embryos in response to modification of incubation temperature
title_short miRNAs regulate acute transcriptional changes in broiler embryos in response to modification of incubation temperature
title_sort mirnas regulate acute transcriptional changes in broiler embryos in response to modification of incubation temperature
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063901/
https://www.ncbi.nlm.nih.gov/pubmed/30054505
http://dx.doi.org/10.1038/s41598-018-29316-7
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