Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

Therapeutic genome editing with Staphylococcus aureus Cas9 (SaCas9) requires a rigorous understanding of its potential off-target activity in the human genome. Here we report a high-throughput screening approach to measure SaCas9 genome editing variation in human cells across a large repertoire of 8...

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Detalles Bibliográficos
Autores principales: Tycko, Josh, Barrera, Luis A., Huston, Nicholas C., Friedland, Ari E., Wu, Xuebing, Gootenberg, Jonathan S., Abudayyeh, Omar O., Myer, Vic E., Wilson, Christopher J., Hsu, Patrick D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063963/
https://www.ncbi.nlm.nih.gov/pubmed/30054474
http://dx.doi.org/10.1038/s41467-018-05391-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063963/
https://www.ncbi.nlm.nih.gov/pubmed/30054474
http://dx.doi.org/10.1038/s41467-018-05391-2

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