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Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells

Therapeutic genome editing with Staphylococcus aureus Cas9 (SaCas9) requires a rigorous understanding of its potential off-target activity in the human genome. Here we report a high-throughput screening approach to measure SaCas9 genome editing variation in human cells across a large repertoire of 8...

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Detalles Bibliográficos
Autores principales:	Tycko, Josh, Barrera, Luis A., Huston, Nicholas C., Friedland, Ari E., Wu, Xuebing, Gootenberg, Jonathan S., Abudayyeh, Omar O., Myer, Vic E., Wilson, Christopher J., Hsu, Patrick D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063963/ https://www.ncbi.nlm.nih.gov/pubmed/30054474 http://dx.doi.org/10.1038/s41467-018-05391-2

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