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Antibacterial Effects of Chitosan, Formocresol and CMCP as Pulpectomy Medicament on Enterococcus faecalis, Staphylococcus aureus and Streptococcus mutans
INTRODUCTION: During pulpectomy of primary teeth, cytotoxic medicaments such as formocresol or camphor mono-chlorophenol (CMCP) are used as medicaments. For the first time it is theorized that chitosan can substitute these traditional materials used in pulpectomy of infectious primary teeth. METHODS...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Iranian Center for Endodontic Research
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064027/ https://www.ncbi.nlm.nih.gov/pubmed/30083204 http://dx.doi.org/10.22037/iej.v13i3.20791 |
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author | Imani, Zhila Imani, Zahra Basir, Leila Shayeste, Mohsen Abbasi Montazeri, Effat Rakhshan, Vahid |
author_facet | Imani, Zhila Imani, Zahra Basir, Leila Shayeste, Mohsen Abbasi Montazeri, Effat Rakhshan, Vahid |
author_sort | Imani, Zhila |
collection | PubMed |
description | INTRODUCTION: During pulpectomy of primary teeth, cytotoxic medicaments such as formocresol or camphor mono-chlorophenol (CMCP) are used as medicaments. For the first time it is theorized that chitosan can substitute these traditional materials used in pulpectomy of infectious primary teeth. METHODS AND MATERIALS: This preliminary in vitro study consisted of two separate phases (n=75), each of which assessed the antibacterial effects of chitosan versus formocresol and CMCP and positive/negative controls (n=15) on three bacteria types [Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, (n=5 per subgroup)]. Phases 1 and 2 concerned respectively with 1- and 7-day effects of these materials. Bacteria were cultured and injected into sterilized canals and colonies were counted. Medicaments were applied and colonies were re-counted after 1 day of treatment (phase 1). Specimens were re-sterilized and re-randomized, and used for phase 2, in which the same procedures were performed for a 7-day period. Effects of agents on bacteria were analyzed statistically (Kruskal-Wallis α=0.05 and Mann-Whitney α=0.017). RESULTS: Treatments reduced bacterial count either after 1 or 7 days (P=0.000). Their effects on different bacteria types were not significant either after 1 or 7 days (P>0.48). Antibacterial efficacies of treatments (indicated by colony reduction) were significantly different, after 7 days (P=0.045). Antibacterial efficacy of chitosan was similar to that of formocresol or CMCP, in both phases [either after 1 or 7 days of treatment (P>0.017). Formocresol and CMCP had similar efficacies in either phase (P>0.017). CONCLUSIONS: This preliminary study confirmed the appropriate antibacterial efficacy of chitosan as a medicament in pulpectomy of infectious primary teeth. |
format | Online Article Text |
id | pubmed-6064027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Iranian Center for Endodontic Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-60640272018-08-06 Antibacterial Effects of Chitosan, Formocresol and CMCP as Pulpectomy Medicament on Enterococcus faecalis, Staphylococcus aureus and Streptococcus mutans Imani, Zhila Imani, Zahra Basir, Leila Shayeste, Mohsen Abbasi Montazeri, Effat Rakhshan, Vahid Iran Endod J Original Article INTRODUCTION: During pulpectomy of primary teeth, cytotoxic medicaments such as formocresol or camphor mono-chlorophenol (CMCP) are used as medicaments. For the first time it is theorized that chitosan can substitute these traditional materials used in pulpectomy of infectious primary teeth. METHODS AND MATERIALS: This preliminary in vitro study consisted of two separate phases (n=75), each of which assessed the antibacterial effects of chitosan versus formocresol and CMCP and positive/negative controls (n=15) on three bacteria types [Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, (n=5 per subgroup)]. Phases 1 and 2 concerned respectively with 1- and 7-day effects of these materials. Bacteria were cultured and injected into sterilized canals and colonies were counted. Medicaments were applied and colonies were re-counted after 1 day of treatment (phase 1). Specimens were re-sterilized and re-randomized, and used for phase 2, in which the same procedures were performed for a 7-day period. Effects of agents on bacteria were analyzed statistically (Kruskal-Wallis α=0.05 and Mann-Whitney α=0.017). RESULTS: Treatments reduced bacterial count either after 1 or 7 days (P=0.000). Their effects on different bacteria types were not significant either after 1 or 7 days (P>0.48). Antibacterial efficacies of treatments (indicated by colony reduction) were significantly different, after 7 days (P=0.045). Antibacterial efficacy of chitosan was similar to that of formocresol or CMCP, in both phases [either after 1 or 7 days of treatment (P>0.017). Formocresol and CMCP had similar efficacies in either phase (P>0.017). CONCLUSIONS: This preliminary study confirmed the appropriate antibacterial efficacy of chitosan as a medicament in pulpectomy of infectious primary teeth. Iranian Center for Endodontic Research 2018 /pmc/articles/PMC6064027/ /pubmed/30083204 http://dx.doi.org/10.22037/iej.v13i3.20791 Text en |
spellingShingle | Original Article Imani, Zhila Imani, Zahra Basir, Leila Shayeste, Mohsen Abbasi Montazeri, Effat Rakhshan, Vahid Antibacterial Effects of Chitosan, Formocresol and CMCP as Pulpectomy Medicament on Enterococcus faecalis, Staphylococcus aureus and Streptococcus mutans |
title | Antibacterial Effects of Chitosan, Formocresol and CMCP as Pulpectomy Medicament on Enterococcus faecalis, Staphylococcus aureus and Streptococcus mutans |
title_full | Antibacterial Effects of Chitosan, Formocresol and CMCP as Pulpectomy Medicament on Enterococcus faecalis, Staphylococcus aureus and Streptococcus mutans |
title_fullStr | Antibacterial Effects of Chitosan, Formocresol and CMCP as Pulpectomy Medicament on Enterococcus faecalis, Staphylococcus aureus and Streptococcus mutans |
title_full_unstemmed | Antibacterial Effects of Chitosan, Formocresol and CMCP as Pulpectomy Medicament on Enterococcus faecalis, Staphylococcus aureus and Streptococcus mutans |
title_short | Antibacterial Effects of Chitosan, Formocresol and CMCP as Pulpectomy Medicament on Enterococcus faecalis, Staphylococcus aureus and Streptococcus mutans |
title_sort | antibacterial effects of chitosan, formocresol and cmcp as pulpectomy medicament on enterococcus faecalis, staphylococcus aureus and streptococcus mutans |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064027/ https://www.ncbi.nlm.nih.gov/pubmed/30083204 http://dx.doi.org/10.22037/iej.v13i3.20791 |
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