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Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes
Kif4a, a member of the kinesin superfamily, has been reported to participate in a series of cellular processes such as chromosome condensation and cytokinesis during mitosis. However, the roles of KIF4a in meiosis are still unknown. In present study we found that the Kif4a protein expression decreas...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
JKL International LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065292/ https://www.ncbi.nlm.nih.gov/pubmed/30090651 http://dx.doi.org/10.14336/AD.2017.0901 |
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author | Tang, Feng Pan, Meng-Hao Lu, Yujie Wan, Xiang Zhang, Yu Sun, Shao-Chen |
author_facet | Tang, Feng Pan, Meng-Hao Lu, Yujie Wan, Xiang Zhang, Yu Sun, Shao-Chen |
author_sort | Tang, Feng |
collection | PubMed |
description | Kif4a, a member of the kinesin superfamily, has been reported to participate in a series of cellular processes such as chromosome condensation and cytokinesis during mitosis. However, the roles of KIF4a in meiosis are still unknown. In present study we found that the Kif4a protein expression decreased in maternal aged mouse oocytes. We then explored the roles of Kif4a in mouse oocyte meiosis by knockdown analysis. Kif4a was enriched at the spindle during mouse oocyte maturation. By specific knock down of the Kif4a using morpholino microinjection, we found that the disruption of Kif4a caused the failure of polar body extrusion. Further analysis indicated that Kif4a might affect the spindle morphology and chromosome alignment in the mouse oocytes, and this might be due to the regulation of tubulin acetylation. Moreover, our results showed that an increased proportion of aneuploidy in the Kif4a knock down oocytes, and this might be due to the loss of kinetochore-microtubule attachment. Taken together, these results suggested that Kif4a possibly regulated mouse oocyte meiosis through its effects on the spindle organization and accurate chromosome segregation, and the loss of Kif4a might be related with aneuploidy of aging oocytes. |
format | Online Article Text |
id | pubmed-6065292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | JKL International LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-60652922018-08-08 Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes Tang, Feng Pan, Meng-Hao Lu, Yujie Wan, Xiang Zhang, Yu Sun, Shao-Chen Aging Dis Orginal Article Kif4a, a member of the kinesin superfamily, has been reported to participate in a series of cellular processes such as chromosome condensation and cytokinesis during mitosis. However, the roles of KIF4a in meiosis are still unknown. In present study we found that the Kif4a protein expression decreased in maternal aged mouse oocytes. We then explored the roles of Kif4a in mouse oocyte meiosis by knockdown analysis. Kif4a was enriched at the spindle during mouse oocyte maturation. By specific knock down of the Kif4a using morpholino microinjection, we found that the disruption of Kif4a caused the failure of polar body extrusion. Further analysis indicated that Kif4a might affect the spindle morphology and chromosome alignment in the mouse oocytes, and this might be due to the regulation of tubulin acetylation. Moreover, our results showed that an increased proportion of aneuploidy in the Kif4a knock down oocytes, and this might be due to the loss of kinetochore-microtubule attachment. Taken together, these results suggested that Kif4a possibly regulated mouse oocyte meiosis through its effects on the spindle organization and accurate chromosome segregation, and the loss of Kif4a might be related with aneuploidy of aging oocytes. JKL International LLC 2018-08-01 /pmc/articles/PMC6065292/ /pubmed/30090651 http://dx.doi.org/10.14336/AD.2017.0901 Text en Copyright: © 2018 Tang et al. http://creativecommons.org/licenses/by/2.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Orginal Article Tang, Feng Pan, Meng-Hao Lu, Yujie Wan, Xiang Zhang, Yu Sun, Shao-Chen Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes |
title | Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes |
title_full | Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes |
title_fullStr | Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes |
title_full_unstemmed | Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes |
title_short | Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes |
title_sort | involvement of kif4a in spindle formation and chromosome segregation in mouse oocytes |
topic | Orginal Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065292/ https://www.ncbi.nlm.nih.gov/pubmed/30090651 http://dx.doi.org/10.14336/AD.2017.0901 |
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