Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes

Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreER(T2) recombinase in mice. Ou...

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Autores principales: Ransom, Ryan C., Foster, Deshka S., Salhotra, Ankit, Jones, Ruth Ellen, Marshall, Clement D., Leavitt, Tripp, Murphy, Matthew P., Moore, Alessandra L., Blackshear, Charles P., Brett, Elizabeth A., Wan, Derrick C., Longaker, Michael T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065311/
https://www.ncbi.nlm.nih.gov/pubmed/30061668
http://dx.doi.org/10.1038/s41467-018-05436-6
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author Ransom, Ryan C.
Foster, Deshka S.
Salhotra, Ankit
Jones, Ruth Ellen
Marshall, Clement D.
Leavitt, Tripp
Murphy, Matthew P.
Moore, Alessandra L.
Blackshear, Charles P.
Brett, Elizabeth A.
Wan, Derrick C.
Longaker, Michael T.
author_facet Ransom, Ryan C.
Foster, Deshka S.
Salhotra, Ankit
Jones, Ruth Ellen
Marshall, Clement D.
Leavitt, Tripp
Murphy, Matthew P.
Moore, Alessandra L.
Blackshear, Charles P.
Brett, Elizabeth A.
Wan, Derrick C.
Longaker, Michael T.
author_sort Ransom, Ryan C.
collection PubMed
description Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreER(T2) recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre drivers in a variety of tissue types, under conditions of homeostasis and post-injury repair, and is highly efficient for lineage tracing and genetic analysis. This methodology is directly and immediately applicable to the developmental biology, stem cell biology and regenerative medicine, and cancer biology fields.
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spelling pubmed-60653112018-07-31 Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes Ransom, Ryan C. Foster, Deshka S. Salhotra, Ankit Jones, Ruth Ellen Marshall, Clement D. Leavitt, Tripp Murphy, Matthew P. Moore, Alessandra L. Blackshear, Charles P. Brett, Elizabeth A. Wan, Derrick C. Longaker, Michael T. Nat Commun Article Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreER(T2) recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre drivers in a variety of tissue types, under conditions of homeostasis and post-injury repair, and is highly efficient for lineage tracing and genetic analysis. This methodology is directly and immediately applicable to the developmental biology, stem cell biology and regenerative medicine, and cancer biology fields. Nature Publishing Group UK 2018-07-30 /pmc/articles/PMC6065311/ /pubmed/30061668 http://dx.doi.org/10.1038/s41467-018-05436-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ransom, Ryan C.
Foster, Deshka S.
Salhotra, Ankit
Jones, Ruth Ellen
Marshall, Clement D.
Leavitt, Tripp
Murphy, Matthew P.
Moore, Alessandra L.
Blackshear, Charles P.
Brett, Elizabeth A.
Wan, Derrick C.
Longaker, Michael T.
Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes
title Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes
title_full Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes
title_fullStr Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes
title_full_unstemmed Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes
title_short Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes
title_sort genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065311/
https://www.ncbi.nlm.nih.gov/pubmed/30061668
http://dx.doi.org/10.1038/s41467-018-05436-6
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