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Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps

We previously reported that chronic rhinosinusitis with nasal polyps (CRSwNP) was subdivided into four chronic rhinosinusitis (CRS) subtypes using the JESREC scoring system. We sought to identify the gene expression profile and biomarkers related with CRSwNP by RNA-sequence. RNA-sequencing was perfo...

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Autores principales: Ninomiya, Takahiro, Noguchi, Emiko, Haruna, Takenori, Hasegawa, Masayo, Yoshida, Takuto, Yamashita, Yukiko, Okano, Mitsuhiro, Yoshida, Naohiro, Haruna, Shinichi, Sakuma, Yasunori, Ohta, Shoichiro, Ono, Junya, Izuhara, Kenji, Okada, Masafumi, Kidoguchi, Masanori, Tokunaga, Takahiro, Okamoto, Masayuki, Kanno, Masafumi, Sakashita, Masafumi, Takabayashi, Tetsuji, Narita, Norihiko, Fujieda, Shigeharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065353/
https://www.ncbi.nlm.nih.gov/pubmed/30061580
http://dx.doi.org/10.1038/s41598-018-29612-2
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author Ninomiya, Takahiro
Noguchi, Emiko
Haruna, Takenori
Hasegawa, Masayo
Yoshida, Takuto
Yamashita, Yukiko
Okano, Mitsuhiro
Yoshida, Naohiro
Haruna, Shinichi
Sakuma, Yasunori
Ohta, Shoichiro
Ono, Junya
Izuhara, Kenji
Okada, Masafumi
Kidoguchi, Masanori
Tokunaga, Takahiro
Okamoto, Masayuki
Kanno, Masafumi
Sakashita, Masafumi
Takabayashi, Tetsuji
Narita, Norihiko
Fujieda, Shigeharu
author_facet Ninomiya, Takahiro
Noguchi, Emiko
Haruna, Takenori
Hasegawa, Masayo
Yoshida, Takuto
Yamashita, Yukiko
Okano, Mitsuhiro
Yoshida, Naohiro
Haruna, Shinichi
Sakuma, Yasunori
Ohta, Shoichiro
Ono, Junya
Izuhara, Kenji
Okada, Masafumi
Kidoguchi, Masanori
Tokunaga, Takahiro
Okamoto, Masayuki
Kanno, Masafumi
Sakashita, Masafumi
Takabayashi, Tetsuji
Narita, Norihiko
Fujieda, Shigeharu
author_sort Ninomiya, Takahiro
collection PubMed
description We previously reported that chronic rhinosinusitis with nasal polyps (CRSwNP) was subdivided into four chronic rhinosinusitis (CRS) subtypes using the JESREC scoring system. We sought to identify the gene expression profile and biomarkers related with CRSwNP by RNA-sequence. RNA-sequencing was performed to identify differentially expressed genes between nasal polyps (NPs) and inferior turbinate mucosa from 6 patients with CRSwNP, and subsequently, quantitative real-time PCR was performed to verify the results. ELISA was performed to identify possible biomarkers for postoperative recurrence. In the RNA-sequencing results, periostin (POSTN) expression was the highest in NP. We focused on POSTN and investigated the protein level of POSTN by immunohistochemistry and ELISA. POSTN was diffusely expressed in moderate and severe eosinophilic CRS using immunohistochemistry, and its staining pattern was associated with the severity of the phenotype of the CRSwNP (P < 0.05). There was a significant difference between the POSTN high/low groups for postoperative recurrence when the cutoff point was set at 115.5 ng/ml (P = 0.0072). Our data suggests that the protein expression level of POSTN was associated with the severity of CRSwNP, and serum POSTN can be a novel biomarker for postoperative recurrence of CRSwNP.
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spelling pubmed-60653532018-08-06 Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps Ninomiya, Takahiro Noguchi, Emiko Haruna, Takenori Hasegawa, Masayo Yoshida, Takuto Yamashita, Yukiko Okano, Mitsuhiro Yoshida, Naohiro Haruna, Shinichi Sakuma, Yasunori Ohta, Shoichiro Ono, Junya Izuhara, Kenji Okada, Masafumi Kidoguchi, Masanori Tokunaga, Takahiro Okamoto, Masayuki Kanno, Masafumi Sakashita, Masafumi Takabayashi, Tetsuji Narita, Norihiko Fujieda, Shigeharu Sci Rep Article We previously reported that chronic rhinosinusitis with nasal polyps (CRSwNP) was subdivided into four chronic rhinosinusitis (CRS) subtypes using the JESREC scoring system. We sought to identify the gene expression profile and biomarkers related with CRSwNP by RNA-sequence. RNA-sequencing was performed to identify differentially expressed genes between nasal polyps (NPs) and inferior turbinate mucosa from 6 patients with CRSwNP, and subsequently, quantitative real-time PCR was performed to verify the results. ELISA was performed to identify possible biomarkers for postoperative recurrence. In the RNA-sequencing results, periostin (POSTN) expression was the highest in NP. We focused on POSTN and investigated the protein level of POSTN by immunohistochemistry and ELISA. POSTN was diffusely expressed in moderate and severe eosinophilic CRS using immunohistochemistry, and its staining pattern was associated with the severity of the phenotype of the CRSwNP (P < 0.05). There was a significant difference between the POSTN high/low groups for postoperative recurrence when the cutoff point was set at 115.5 ng/ml (P = 0.0072). Our data suggests that the protein expression level of POSTN was associated with the severity of CRSwNP, and serum POSTN can be a novel biomarker for postoperative recurrence of CRSwNP. Nature Publishing Group UK 2018-07-30 /pmc/articles/PMC6065353/ /pubmed/30061580 http://dx.doi.org/10.1038/s41598-018-29612-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ninomiya, Takahiro
Noguchi, Emiko
Haruna, Takenori
Hasegawa, Masayo
Yoshida, Takuto
Yamashita, Yukiko
Okano, Mitsuhiro
Yoshida, Naohiro
Haruna, Shinichi
Sakuma, Yasunori
Ohta, Shoichiro
Ono, Junya
Izuhara, Kenji
Okada, Masafumi
Kidoguchi, Masanori
Tokunaga, Takahiro
Okamoto, Masayuki
Kanno, Masafumi
Sakashita, Masafumi
Takabayashi, Tetsuji
Narita, Norihiko
Fujieda, Shigeharu
Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps
title Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps
title_full Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps
title_fullStr Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps
title_full_unstemmed Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps
title_short Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps
title_sort periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065353/
https://www.ncbi.nlm.nih.gov/pubmed/30061580
http://dx.doi.org/10.1038/s41598-018-29612-2
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