Three newly identified Immediate Early Genes of Bovine herpesvirus 1 lack the characteristic Octamer binding motif- 1

Only three immediate early genes (IE) BICP0, BICP4 and BICP22 of Bovine herpesvirus 1 (BoHV-1) are known. These genes are expressed coordinately and their promoters are well characterized. We provide evidence for expression of three additional IE genes of BoHV-1 i.e. UL21, UL33 and UL34. These genes...

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Autores principales: Pokhriyal, Mayank, Ratta, Barkha, Yadav, Brijesh Singh, Kumar, Ajay, Saxena, Meeta, Verma, Om Prakash, Sharma, Bhaskar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065388/
https://www.ncbi.nlm.nih.gov/pubmed/30061689
http://dx.doi.org/10.1038/s41598-018-29490-8
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author Pokhriyal, Mayank
Ratta, Barkha
Yadav, Brijesh Singh
Kumar, Ajay
Saxena, Meeta
Verma, Om Prakash
Sharma, Bhaskar
author_facet Pokhriyal, Mayank
Ratta, Barkha
Yadav, Brijesh Singh
Kumar, Ajay
Saxena, Meeta
Verma, Om Prakash
Sharma, Bhaskar
author_sort Pokhriyal, Mayank
collection PubMed
description Only three immediate early genes (IE) BICP0, BICP4 and BICP22 of Bovine herpesvirus 1 (BoHV-1) are known. These genes are expressed coordinately and their promoters are well characterized. We provide evidence for expression of three additional IE genes of BoHV-1 i.e. UL21, UL33 and UL34. These genes are expressed in the presence of cycloheximide (CH) at the same time as known IE genes. Surprisingly, the promoters of newly identified IE genes (UL21, UL33, UL34) lack the OCT-1 binding site, a considered site of transactivation of the BoHV-1 IE genes. The other difference in the promoters of the newly identified IE genes is the presence of TATA box at near optimal site. However, all the IE genes have similar spatial placements of C/EBPα, DPE and INR elements.
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spelling pubmed-60653882018-08-06 Three newly identified Immediate Early Genes of Bovine herpesvirus 1 lack the characteristic Octamer binding motif- 1 Pokhriyal, Mayank Ratta, Barkha Yadav, Brijesh Singh Kumar, Ajay Saxena, Meeta Verma, Om Prakash Sharma, Bhaskar Sci Rep Article Only three immediate early genes (IE) BICP0, BICP4 and BICP22 of Bovine herpesvirus 1 (BoHV-1) are known. These genes are expressed coordinately and their promoters are well characterized. We provide evidence for expression of three additional IE genes of BoHV-1 i.e. UL21, UL33 and UL34. These genes are expressed in the presence of cycloheximide (CH) at the same time as known IE genes. Surprisingly, the promoters of newly identified IE genes (UL21, UL33, UL34) lack the OCT-1 binding site, a considered site of transactivation of the BoHV-1 IE genes. The other difference in the promoters of the newly identified IE genes is the presence of TATA box at near optimal site. However, all the IE genes have similar spatial placements of C/EBPα, DPE and INR elements. Nature Publishing Group UK 2018-07-30 /pmc/articles/PMC6065388/ /pubmed/30061689 http://dx.doi.org/10.1038/s41598-018-29490-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pokhriyal, Mayank
Ratta, Barkha
Yadav, Brijesh Singh
Kumar, Ajay
Saxena, Meeta
Verma, Om Prakash
Sharma, Bhaskar
Three newly identified Immediate Early Genes of Bovine herpesvirus 1 lack the characteristic Octamer binding motif- 1
title Three newly identified Immediate Early Genes of Bovine herpesvirus 1 lack the characteristic Octamer binding motif- 1
title_full Three newly identified Immediate Early Genes of Bovine herpesvirus 1 lack the characteristic Octamer binding motif- 1
title_fullStr Three newly identified Immediate Early Genes of Bovine herpesvirus 1 lack the characteristic Octamer binding motif- 1
title_full_unstemmed Three newly identified Immediate Early Genes of Bovine herpesvirus 1 lack the characteristic Octamer binding motif- 1
title_short Three newly identified Immediate Early Genes of Bovine herpesvirus 1 lack the characteristic Octamer binding motif- 1
title_sort three newly identified immediate early genes of bovine herpesvirus 1 lack the characteristic octamer binding motif- 1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065388/
https://www.ncbi.nlm.nih.gov/pubmed/30061689
http://dx.doi.org/10.1038/s41598-018-29490-8
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