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Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses
Among the strategies targeting vector control, the exploitation of the endosymbiont Wolbachia to produce sterile males and/or invasive females with reduced vector competence seems to be promising. A new Aedes albopictus transinfection (ARwP-M) was generated by introducing wMel Wolbachia in the ARwP...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066253/ https://www.ncbi.nlm.nih.gov/pubmed/30020933 http://dx.doi.org/10.1371/journal.pntd.0006626 |
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author | Moretti, Riccardo Yen, Pei-Shi Houé, Vincent Lampazzi, Elena Desiderio, Angiola Failloux, Anna-Bella Calvitti, Maurizio |
author_facet | Moretti, Riccardo Yen, Pei-Shi Houé, Vincent Lampazzi, Elena Desiderio, Angiola Failloux, Anna-Bella Calvitti, Maurizio |
author_sort | Moretti, Riccardo |
collection | PubMed |
description | Among the strategies targeting vector control, the exploitation of the endosymbiont Wolbachia to produce sterile males and/or invasive females with reduced vector competence seems to be promising. A new Aedes albopictus transinfection (ARwP-M) was generated by introducing wMel Wolbachia in the ARwP line which had been established previously by replacing wAlbA and wAlbB Wolbachia with the wPip strain. Various infection and fitness parameters were studied by comparing ARwP-M, ARwP and wild-type (S(ANG) population) Ae. albopictus sharing the same genetic background. Moreover, the vector competence of ARwP-M related to chikungunya, dengue and zika viruses was evaluated in comparison with ARwP. ARwP-M showed a 100% rate of maternal inheritance of wMel and wPip Wolbachia. Survival, female fecundity and egg fertility did not show to differ between the three Ae. albopictus lines. Crosses between ARwP-M males and S(ANG) females were fully unfertile regardless of male age while egg hatch in reverse crosses increased from 0 to about 17% with S(ANG) males aging from 3 to 17 days. When competing with S(ANG) males for S(ANG) females, ARwP-M males induced a level of sterility significantly higher than that expected for an equal mating competitiveness (mean Fried index of 1.71 instead of 1). The overall Wolbachia density in ARwP-M females was about 15 fold higher than in ARwP, mostly due to the wMel infection. This feature corresponded to a strongly reduced vector competence for chikungunya and dengue viruses (in both cases, 5 and 0% rates of transmission at 14 and 21 days post infection) with respect to ARwP females. Results regarding Zika virus did not highlight significant differences between ARwP-M and ARwP. However, none of the tested ARwP-M females was capable at transmitting ZIKV. These findings are expected to promote the exploitation of Wolbachia to suppress the wild-type Ae. albopictus populations. |
format | Online Article Text |
id | pubmed-6066253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60662532018-08-13 Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses Moretti, Riccardo Yen, Pei-Shi Houé, Vincent Lampazzi, Elena Desiderio, Angiola Failloux, Anna-Bella Calvitti, Maurizio PLoS Negl Trop Dis Research Article Among the strategies targeting vector control, the exploitation of the endosymbiont Wolbachia to produce sterile males and/or invasive females with reduced vector competence seems to be promising. A new Aedes albopictus transinfection (ARwP-M) was generated by introducing wMel Wolbachia in the ARwP line which had been established previously by replacing wAlbA and wAlbB Wolbachia with the wPip strain. Various infection and fitness parameters were studied by comparing ARwP-M, ARwP and wild-type (S(ANG) population) Ae. albopictus sharing the same genetic background. Moreover, the vector competence of ARwP-M related to chikungunya, dengue and zika viruses was evaluated in comparison with ARwP. ARwP-M showed a 100% rate of maternal inheritance of wMel and wPip Wolbachia. Survival, female fecundity and egg fertility did not show to differ between the three Ae. albopictus lines. Crosses between ARwP-M males and S(ANG) females were fully unfertile regardless of male age while egg hatch in reverse crosses increased from 0 to about 17% with S(ANG) males aging from 3 to 17 days. When competing with S(ANG) males for S(ANG) females, ARwP-M males induced a level of sterility significantly higher than that expected for an equal mating competitiveness (mean Fried index of 1.71 instead of 1). The overall Wolbachia density in ARwP-M females was about 15 fold higher than in ARwP, mostly due to the wMel infection. This feature corresponded to a strongly reduced vector competence for chikungunya and dengue viruses (in both cases, 5 and 0% rates of transmission at 14 and 21 days post infection) with respect to ARwP females. Results regarding Zika virus did not highlight significant differences between ARwP-M and ARwP. However, none of the tested ARwP-M females was capable at transmitting ZIKV. These findings are expected to promote the exploitation of Wolbachia to suppress the wild-type Ae. albopictus populations. Public Library of Science 2018-07-18 /pmc/articles/PMC6066253/ /pubmed/30020933 http://dx.doi.org/10.1371/journal.pntd.0006626 Text en © 2018 Moretti et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Moretti, Riccardo Yen, Pei-Shi Houé, Vincent Lampazzi, Elena Desiderio, Angiola Failloux, Anna-Bella Calvitti, Maurizio Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses |
title | Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses |
title_full | Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses |
title_fullStr | Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses |
title_full_unstemmed | Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses |
title_short | Combining Wolbachia-induced sterility and virus protection to fight Aedes albopictus-borne viruses |
title_sort | combining wolbachia-induced sterility and virus protection to fight aedes albopictus-borne viruses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066253/ https://www.ncbi.nlm.nih.gov/pubmed/30020933 http://dx.doi.org/10.1371/journal.pntd.0006626 |
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