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Host Factor Nucleoporin 93 Is Involved in the Nuclear Export of Influenza Virus RNA

Influenza virus replication relies on the functions of host factors. In our previous study, we identified host factors involved in virus replication and began analyses of their roles in this process. In this study, we focused on Nucleoporin 93 (NUP93) and revealed its importance in influenza virus r...

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Autores principales: Furusawa, Yuri, Yamada, Shinya, Kawaoka, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066526/
https://www.ncbi.nlm.nih.gov/pubmed/30087672
http://dx.doi.org/10.3389/fmicb.2018.01675
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author Furusawa, Yuri
Yamada, Shinya
Kawaoka, Yoshihiro
author_facet Furusawa, Yuri
Yamada, Shinya
Kawaoka, Yoshihiro
author_sort Furusawa, Yuri
collection PubMed
description Influenza virus replication relies on the functions of host factors. In our previous study, we identified host factors involved in virus replication and began analyses of their roles in this process. In this study, we focused on Nucleoporin 93 (NUP93) and revealed its importance in influenza virus replication. NUP93 knockdown mediated by siRNAs reduced viral replication and decreased the efficiency of the early step of the viral life cycle. NUP93 did not appear to be important for virus binding, internalization, or the nuclear import of viral ribonucleoprotein (vRNP); however, in NUP93-depleted cells, viral RNA accumulated in the nucleus. These results suggest that NUP93 is involved in the nuclear export of viral RNA.
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spelling pubmed-60665262018-08-07 Host Factor Nucleoporin 93 Is Involved in the Nuclear Export of Influenza Virus RNA Furusawa, Yuri Yamada, Shinya Kawaoka, Yoshihiro Front Microbiol Microbiology Influenza virus replication relies on the functions of host factors. In our previous study, we identified host factors involved in virus replication and began analyses of their roles in this process. In this study, we focused on Nucleoporin 93 (NUP93) and revealed its importance in influenza virus replication. NUP93 knockdown mediated by siRNAs reduced viral replication and decreased the efficiency of the early step of the viral life cycle. NUP93 did not appear to be important for virus binding, internalization, or the nuclear import of viral ribonucleoprotein (vRNP); however, in NUP93-depleted cells, viral RNA accumulated in the nucleus. These results suggest that NUP93 is involved in the nuclear export of viral RNA. Frontiers Media S.A. 2018-07-24 /pmc/articles/PMC6066526/ /pubmed/30087672 http://dx.doi.org/10.3389/fmicb.2018.01675 Text en Copyright © 2018 Furusawa, Yamada and Kawaoka. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Furusawa, Yuri
Yamada, Shinya
Kawaoka, Yoshihiro
Host Factor Nucleoporin 93 Is Involved in the Nuclear Export of Influenza Virus RNA
title Host Factor Nucleoporin 93 Is Involved in the Nuclear Export of Influenza Virus RNA
title_full Host Factor Nucleoporin 93 Is Involved in the Nuclear Export of Influenza Virus RNA
title_fullStr Host Factor Nucleoporin 93 Is Involved in the Nuclear Export of Influenza Virus RNA
title_full_unstemmed Host Factor Nucleoporin 93 Is Involved in the Nuclear Export of Influenza Virus RNA
title_short Host Factor Nucleoporin 93 Is Involved in the Nuclear Export of Influenza Virus RNA
title_sort host factor nucleoporin 93 is involved in the nuclear export of influenza virus rna
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066526/
https://www.ncbi.nlm.nih.gov/pubmed/30087672
http://dx.doi.org/10.3389/fmicb.2018.01675
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