A tRNA-derived small RNA regulates ribosome biogenesis

tRNA-derived small RNAs (tsRNAs; also called tRNA-derived fragments (tRFs)) are an abundant class of small non-coding RNAs whose biological roles are not well defined. We show that inhibition of a specific tsRNA, LeuCAG3′tsRNA, induces apoptosis in rapidly dividing cells in vitro and in a patient-de...

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Autores principales: Kim, Hak Kyun, Fuchs, Gabriele, Wang, Shengchun, Wei, Wei, Zhang, Yue, Park, Hyesuk, Roy-Chaudhuri, Biswajoy, Li, Pan, Xu, Jianpeng, Chu, Kirk, Zhang, Feijie, Chua, Mei-Sze, So, Samuel, Zhang, Qiangfeng Cliff, Sarnow, Peter, Kay, Mark A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066594/
https://www.ncbi.nlm.nih.gov/pubmed/29186115
http://dx.doi.org/10.1038/nature25005
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author Kim, Hak Kyun
Fuchs, Gabriele
Wang, Shengchun
Wei, Wei
Zhang, Yue
Park, Hyesuk
Roy-Chaudhuri, Biswajoy
Li, Pan
Xu, Jianpeng
Chu, Kirk
Zhang, Feijie
Chua, Mei-Sze
So, Samuel
Zhang, Qiangfeng Cliff
Sarnow, Peter
Kay, Mark A.
author_facet Kim, Hak Kyun
Fuchs, Gabriele
Wang, Shengchun
Wei, Wei
Zhang, Yue
Park, Hyesuk
Roy-Chaudhuri, Biswajoy
Li, Pan
Xu, Jianpeng
Chu, Kirk
Zhang, Feijie
Chua, Mei-Sze
So, Samuel
Zhang, Qiangfeng Cliff
Sarnow, Peter
Kay, Mark A.
author_sort Kim, Hak Kyun
collection PubMed
description tRNA-derived small RNAs (tsRNAs; also called tRNA-derived fragments (tRFs)) are an abundant class of small non-coding RNAs whose biological roles are not well defined. We show that inhibition of a specific tsRNA, LeuCAG3′tsRNA, induces apoptosis in rapidly dividing cells in vitro and in a patient-derived orthotopic hepatocellular carcinoma model in mice. This tsRNA binds at least two ribosomal protein mRNAs (for RPS28 and RPS15) to enhance their translation. Reduction of RPS28 mRNA translation blocks pre-18S ribosomal RNA processing, resulting in a decrease in the number of 40S ribosomal subunits. These data establish another post-transcriptional mechanism that can fine-tune gene expression during different physiological states and provide a potential new target for treating cancer.
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spelling pubmed-60665942018-07-31 A tRNA-derived small RNA regulates ribosome biogenesis Kim, Hak Kyun Fuchs, Gabriele Wang, Shengchun Wei, Wei Zhang, Yue Park, Hyesuk Roy-Chaudhuri, Biswajoy Li, Pan Xu, Jianpeng Chu, Kirk Zhang, Feijie Chua, Mei-Sze So, Samuel Zhang, Qiangfeng Cliff Sarnow, Peter Kay, Mark A. Nature Article tRNA-derived small RNAs (tsRNAs; also called tRNA-derived fragments (tRFs)) are an abundant class of small non-coding RNAs whose biological roles are not well defined. We show that inhibition of a specific tsRNA, LeuCAG3′tsRNA, induces apoptosis in rapidly dividing cells in vitro and in a patient-derived orthotopic hepatocellular carcinoma model in mice. This tsRNA binds at least two ribosomal protein mRNAs (for RPS28 and RPS15) to enhance their translation. Reduction of RPS28 mRNA translation blocks pre-18S ribosomal RNA processing, resulting in a decrease in the number of 40S ribosomal subunits. These data establish another post-transcriptional mechanism that can fine-tune gene expression during different physiological states and provide a potential new target for treating cancer. 2017-11-29 2017-12-07 /pmc/articles/PMC6066594/ /pubmed/29186115 http://dx.doi.org/10.1038/nature25005 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms Reprints and permissions information is available at www.nature.com/reprints (http://www.nature.com/reprints) .
spellingShingle Article
Kim, Hak Kyun
Fuchs, Gabriele
Wang, Shengchun
Wei, Wei
Zhang, Yue
Park, Hyesuk
Roy-Chaudhuri, Biswajoy
Li, Pan
Xu, Jianpeng
Chu, Kirk
Zhang, Feijie
Chua, Mei-Sze
So, Samuel
Zhang, Qiangfeng Cliff
Sarnow, Peter
Kay, Mark A.
A tRNA-derived small RNA regulates ribosome biogenesis
title A tRNA-derived small RNA regulates ribosome biogenesis
title_full A tRNA-derived small RNA regulates ribosome biogenesis
title_fullStr A tRNA-derived small RNA regulates ribosome biogenesis
title_full_unstemmed A tRNA-derived small RNA regulates ribosome biogenesis
title_short A tRNA-derived small RNA regulates ribosome biogenesis
title_sort trna-derived small rna regulates ribosome biogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066594/
https://www.ncbi.nlm.nih.gov/pubmed/29186115
http://dx.doi.org/10.1038/nature25005
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