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Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design

Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study...

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Detalles Bibliográficos
Autores principales: Moghannem, Saad A.M., Farag, Mohamed M.S., Shehab, Amr M., Azab, Mohamed S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066745/
https://www.ncbi.nlm.nih.gov/pubmed/29449173
http://dx.doi.org/10.1016/j.bjm.2017.05.012
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author Moghannem, Saad A.M.
Farag, Mohamed M.S.
Shehab, Amr M.
Azab, Mohamed S.
author_facet Moghannem, Saad A.M.
Farag, Mohamed M.S.
Shehab, Amr M.
Azab, Mohamed S.
author_sort Moghannem, Saad A.M.
collection PubMed
description Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett–Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters. Box–Behnken RSM indicated that; the optimum concentration for each parameter was 12% (w/v) for molasses, 6 g/L yeast extract and 30 °C for incubation temperature. The most potent bacterial isolate was identified as Bacillus velezensis KY498625. After production, EPS was extracted, purified using DEAE-cellulose, identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and gas chromatography–mass spectroscopy (GC–MS). The result indicated that; it has molecular weight 1.14 × 10(5) D consisting of glucose, mannose and galactose.
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spelling pubmed-60667452018-08-01 Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design Moghannem, Saad A.M. Farag, Mohamed M.S. Shehab, Amr M. Azab, Mohamed S. Braz J Microbiol Environmental Microbiology Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett–Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters. Box–Behnken RSM indicated that; the optimum concentration for each parameter was 12% (w/v) for molasses, 6 g/L yeast extract and 30 °C for incubation temperature. The most potent bacterial isolate was identified as Bacillus velezensis KY498625. After production, EPS was extracted, purified using DEAE-cellulose, identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and gas chromatography–mass spectroscopy (GC–MS). The result indicated that; it has molecular weight 1.14 × 10(5) D consisting of glucose, mannose and galactose. Brazilian Society of Microbiology 2018-01-18 /pmc/articles/PMC6066745/ /pubmed/29449173 http://dx.doi.org/10.1016/j.bjm.2017.05.012 Text en © 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Environmental Microbiology
Moghannem, Saad A.M.
Farag, Mohamed M.S.
Shehab, Amr M.
Azab, Mohamed S.
Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title_full Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title_fullStr Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title_full_unstemmed Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title_short Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title_sort exopolysaccharide production from bacillus velezensis ky471306 using statistical experimental design
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066745/
https://www.ncbi.nlm.nih.gov/pubmed/29449173
http://dx.doi.org/10.1016/j.bjm.2017.05.012
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