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Characterization of Mucus-Related Properties of Streptococcus thermophilus: From Adhesion to Induction
Mucus is a major component of the intestinal barrier involved both in the protection of the host and the fitness of commensals of the gut. Streptococcus thermophilus is consumed world-wide in fermented dairy products and is also recognized as a probiotic, as its consumption is associated with improv...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6067005/ https://www.ncbi.nlm.nih.gov/pubmed/30087622 http://dx.doi.org/10.3389/fphys.2018.00980 |
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author | Fernandez, Neïké Wrzosek, Laura Radziwill-Bienkowska, Joanna M. Ringot-Destrez, Belinda Duviau, Marie-Pierre Noordine, Marie-Louise Laroute, Valérie Robert, Véronique Cherbuy, Claire Daveran-Mingot, Marie-Line Cocaign-Bousquet, Muriel Léonard, Renaud Robbe-Masselot, Catherine Rul, Françoise Ogier-Denis, Eric Thomas, Muriel Mercier-Bonin, Muriel |
author_facet | Fernandez, Neïké Wrzosek, Laura Radziwill-Bienkowska, Joanna M. Ringot-Destrez, Belinda Duviau, Marie-Pierre Noordine, Marie-Louise Laroute, Valérie Robert, Véronique Cherbuy, Claire Daveran-Mingot, Marie-Line Cocaign-Bousquet, Muriel Léonard, Renaud Robbe-Masselot, Catherine Rul, Françoise Ogier-Denis, Eric Thomas, Muriel Mercier-Bonin, Muriel |
author_sort | Fernandez, Neïké |
collection | PubMed |
description | Mucus is a major component of the intestinal barrier involved both in the protection of the host and the fitness of commensals of the gut. Streptococcus thermophilus is consumed world-wide in fermented dairy products and is also recognized as a probiotic, as its consumption is associated with improved lactose digestion. We determined the overall effect of S. thermophilus on the mucus by evaluating its ability to adhere, degrade, modify, or induce the production of mucus and/or mucins. Adhesion was analyzed in vitro using two types of mucins (from pig or human biopsies) and mucus-producing intestinal HT29-MTX cells. The induction of mucus was characterized in two different rodent models, in which S. thermophilus is the unique bacterial species in the digestive tract or transited as a sub-dominant bacterium through a complex microbiota. S. thermophilus LMD-9 and LMG18311 strains did not grow in sugars used to form mucins as the sole carbon source and displayed weak binding to mucus/mucins relative to the highly adhesive TIL448 Lactococcus lactis. The presence of S. thermophilus as the unique bacteria in the digestive tract of gnotobiotic rats led to accumulation of lactate and increased the number of Alcian-Blue positive goblet cells and the amount of the mucus-inducer KLF4 transcription factor. Lactate significantly increased KLF4 protein levels in HT29-MTX cells. Introduction of S. thermophilus via transit as a sub-dominant bacterium (10(3) CFU/g feces) in a complex endogenous microbiota resulted in a slight increase in lactate levels in the digestive tract, no induction of overall mucus production, and moderate induction of sulfated mucin production. We thus show that although S. thermophilus is a poor mucus-adhesive bacterium, it can promote mucus pathway at least in part by producing lactate in the digestive tract. |
format | Online Article Text |
id | pubmed-6067005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60670052018-08-07 Characterization of Mucus-Related Properties of Streptococcus thermophilus: From Adhesion to Induction Fernandez, Neïké Wrzosek, Laura Radziwill-Bienkowska, Joanna M. Ringot-Destrez, Belinda Duviau, Marie-Pierre Noordine, Marie-Louise Laroute, Valérie Robert, Véronique Cherbuy, Claire Daveran-Mingot, Marie-Line Cocaign-Bousquet, Muriel Léonard, Renaud Robbe-Masselot, Catherine Rul, Françoise Ogier-Denis, Eric Thomas, Muriel Mercier-Bonin, Muriel Front Physiol Physiology Mucus is a major component of the intestinal barrier involved both in the protection of the host and the fitness of commensals of the gut. Streptococcus thermophilus is consumed world-wide in fermented dairy products and is also recognized as a probiotic, as its consumption is associated with improved lactose digestion. We determined the overall effect of S. thermophilus on the mucus by evaluating its ability to adhere, degrade, modify, or induce the production of mucus and/or mucins. Adhesion was analyzed in vitro using two types of mucins (from pig or human biopsies) and mucus-producing intestinal HT29-MTX cells. The induction of mucus was characterized in two different rodent models, in which S. thermophilus is the unique bacterial species in the digestive tract or transited as a sub-dominant bacterium through a complex microbiota. S. thermophilus LMD-9 and LMG18311 strains did not grow in sugars used to form mucins as the sole carbon source and displayed weak binding to mucus/mucins relative to the highly adhesive TIL448 Lactococcus lactis. The presence of S. thermophilus as the unique bacteria in the digestive tract of gnotobiotic rats led to accumulation of lactate and increased the number of Alcian-Blue positive goblet cells and the amount of the mucus-inducer KLF4 transcription factor. Lactate significantly increased KLF4 protein levels in HT29-MTX cells. Introduction of S. thermophilus via transit as a sub-dominant bacterium (10(3) CFU/g feces) in a complex endogenous microbiota resulted in a slight increase in lactate levels in the digestive tract, no induction of overall mucus production, and moderate induction of sulfated mucin production. We thus show that although S. thermophilus is a poor mucus-adhesive bacterium, it can promote mucus pathway at least in part by producing lactate in the digestive tract. Frontiers Media S.A. 2018-07-24 /pmc/articles/PMC6067005/ /pubmed/30087622 http://dx.doi.org/10.3389/fphys.2018.00980 Text en Copyright © 2018 Fernandez, Wrzosek, Radziwill-Bienkowska, Ringot-Destrez, Duviau, Noordine, Laroute, Robert, Cherbuy, Daveran-Mingot, Cocaign-Bousquet, Léonard, Robbe-Masselot, Rul, Ogier-Denis, Thomas and Mercier-Bonin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Fernandez, Neïké Wrzosek, Laura Radziwill-Bienkowska, Joanna M. Ringot-Destrez, Belinda Duviau, Marie-Pierre Noordine, Marie-Louise Laroute, Valérie Robert, Véronique Cherbuy, Claire Daveran-Mingot, Marie-Line Cocaign-Bousquet, Muriel Léonard, Renaud Robbe-Masselot, Catherine Rul, Françoise Ogier-Denis, Eric Thomas, Muriel Mercier-Bonin, Muriel Characterization of Mucus-Related Properties of Streptococcus thermophilus: From Adhesion to Induction |
title | Characterization of Mucus-Related Properties of Streptococcus thermophilus: From Adhesion to Induction |
title_full | Characterization of Mucus-Related Properties of Streptococcus thermophilus: From Adhesion to Induction |
title_fullStr | Characterization of Mucus-Related Properties of Streptococcus thermophilus: From Adhesion to Induction |
title_full_unstemmed | Characterization of Mucus-Related Properties of Streptococcus thermophilus: From Adhesion to Induction |
title_short | Characterization of Mucus-Related Properties of Streptococcus thermophilus: From Adhesion to Induction |
title_sort | characterization of mucus-related properties of streptococcus thermophilus: from adhesion to induction |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6067005/ https://www.ncbi.nlm.nih.gov/pubmed/30087622 http://dx.doi.org/10.3389/fphys.2018.00980 |
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