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Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples

Bacterial contamination of water sources (e.g., lakes, rivers and springs) from waterborne bacteria is a crucial water safety issue and its prevention is of the utmost significance since it threatens the health and well-being of wildlife, livestock, and human populations and can lead to serious illn...

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Autores principales: Malec, Anna, Kokkinis, Georgios, Haiden, Christoph, Giouroudi, Ioanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068504/
https://www.ncbi.nlm.nih.gov/pubmed/30002348
http://dx.doi.org/10.3390/s18072250
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author Malec, Anna
Kokkinis, Georgios
Haiden, Christoph
Giouroudi, Ioanna
author_facet Malec, Anna
Kokkinis, Georgios
Haiden, Christoph
Giouroudi, Ioanna
author_sort Malec, Anna
collection PubMed
description Bacterial contamination of water sources (e.g., lakes, rivers and springs) from waterborne bacteria is a crucial water safety issue and its prevention is of the utmost significance since it threatens the health and well-being of wildlife, livestock, and human populations and can lead to serious illness and even death. Rapid and multiplexed measurement of such waterborne pathogens is vital and the challenge is to instantly detect in these liquid samples different types of pathogens with high sensitivity and specificity. In this work, we propose a biosensing system in which the bacteria are labelled with streptavidin coated magnetic markers (MPs—magnetic particles) forming compounds (MLBs—magnetically labelled bacteria). Video microscopy in combination with a particle tracking software are used for their detection and quantification. When the liquid containing the MLBs is introduced into the developed, microfluidic platform, the MLBs are accelerated towards the outlet by means of a magnetic field gradient generated by integrated microconductors, which are sequentially switched ON and OFF by a microcontroller. The velocities of the MLBs and that of reference MPs, suspended in the same liquid in a parallel reference microfluidic channel, are calculated and compared in real time by a digital camera mounted on a conventional optical microscope in combination with a particle trajectory tracking software. The MLBs will be slower than the reference MPs due to the enhanced Stokes’ drag force exerted on them, resulting from their greater volume and altered hydrodynamic shape. The results of the investigation showed that the parameters obtained from this method emerged as reliable predictors for E. coli concentrations.
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spelling pubmed-60685042018-08-07 Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples Malec, Anna Kokkinis, Georgios Haiden, Christoph Giouroudi, Ioanna Sensors (Basel) Article Bacterial contamination of water sources (e.g., lakes, rivers and springs) from waterborne bacteria is a crucial water safety issue and its prevention is of the utmost significance since it threatens the health and well-being of wildlife, livestock, and human populations and can lead to serious illness and even death. Rapid and multiplexed measurement of such waterborne pathogens is vital and the challenge is to instantly detect in these liquid samples different types of pathogens with high sensitivity and specificity. In this work, we propose a biosensing system in which the bacteria are labelled with streptavidin coated magnetic markers (MPs—magnetic particles) forming compounds (MLBs—magnetically labelled bacteria). Video microscopy in combination with a particle tracking software are used for their detection and quantification. When the liquid containing the MLBs is introduced into the developed, microfluidic platform, the MLBs are accelerated towards the outlet by means of a magnetic field gradient generated by integrated microconductors, which are sequentially switched ON and OFF by a microcontroller. The velocities of the MLBs and that of reference MPs, suspended in the same liquid in a parallel reference microfluidic channel, are calculated and compared in real time by a digital camera mounted on a conventional optical microscope in combination with a particle trajectory tracking software. The MLBs will be slower than the reference MPs due to the enhanced Stokes’ drag force exerted on them, resulting from their greater volume and altered hydrodynamic shape. The results of the investigation showed that the parameters obtained from this method emerged as reliable predictors for E. coli concentrations. MDPI 2018-07-12 /pmc/articles/PMC6068504/ /pubmed/30002348 http://dx.doi.org/10.3390/s18072250 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Malec, Anna
Kokkinis, Georgios
Haiden, Christoph
Giouroudi, Ioanna
Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples
title Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples
title_full Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples
title_fullStr Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples
title_full_unstemmed Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples
title_short Biosensing System for Concentration Quantification of Magnetically Labeled E. coli in Water Samples
title_sort biosensing system for concentration quantification of magnetically labeled e. coli in water samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068504/
https://www.ncbi.nlm.nih.gov/pubmed/30002348
http://dx.doi.org/10.3390/s18072250
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