Quantitative Investigation for the Dielectrophoretic Effect of Fluorescent Dyes at Single-Cell Resolution

[Image: see text] Most of the microscopy-based, quantitative assays rely on fluorescent dyes. In this study, we investigated the impact of fluorescent dyes on the dielectrophoretic response of the mammalian cells. The dielectrophoretic measurements were performed to quantify whether the fluorescent...

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Autores principales: Yildizhan, Yagmur, Gogebakan, Umut B., Altay, Alara, Islam, Monsur, Martinez-Duarte, Rodrigo, Elitas, Meltem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068594/
https://www.ncbi.nlm.nih.gov/pubmed/30087910
http://dx.doi.org/10.1021/acsomega.8b00541
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author Yildizhan, Yagmur
Gogebakan, Umut B.
Altay, Alara
Islam, Monsur
Martinez-Duarte, Rodrigo
Elitas, Meltem
author_facet Yildizhan, Yagmur
Gogebakan, Umut B.
Altay, Alara
Islam, Monsur
Martinez-Duarte, Rodrigo
Elitas, Meltem
author_sort Yildizhan, Yagmur
collection PubMed
description [Image: see text] Most of the microscopy-based, quantitative assays rely on fluorescent dyes. In this study, we investigated the impact of fluorescent dyes on the dielectrophoretic response of the mammalian cells. The dielectrophoretic measurements were performed to quantify whether the fluorescent dyes alter the dielectrophoretic properties of the cells at single-cell resolution. Our results present that when 10 V(pp) electric field is applied, the fluorescent-labeled cells experienced the crossover frequency at 8–10 kHz, whereas the label-free cells exhibited at 16–18 kHz.
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spelling pubmed-60685942018-08-05 Quantitative Investigation for the Dielectrophoretic Effect of Fluorescent Dyes at Single-Cell Resolution Yildizhan, Yagmur Gogebakan, Umut B. Altay, Alara Islam, Monsur Martinez-Duarte, Rodrigo Elitas, Meltem ACS Omega [Image: see text] Most of the microscopy-based, quantitative assays rely on fluorescent dyes. In this study, we investigated the impact of fluorescent dyes on the dielectrophoretic response of the mammalian cells. The dielectrophoretic measurements were performed to quantify whether the fluorescent dyes alter the dielectrophoretic properties of the cells at single-cell resolution. Our results present that when 10 V(pp) electric field is applied, the fluorescent-labeled cells experienced the crossover frequency at 8–10 kHz, whereas the label-free cells exhibited at 16–18 kHz. American Chemical Society 2018-07-03 /pmc/articles/PMC6068594/ /pubmed/30087910 http://dx.doi.org/10.1021/acsomega.8b00541 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Yildizhan, Yagmur
Gogebakan, Umut B.
Altay, Alara
Islam, Monsur
Martinez-Duarte, Rodrigo
Elitas, Meltem
Quantitative Investigation for the Dielectrophoretic Effect of Fluorescent Dyes at Single-Cell Resolution
title Quantitative Investigation for the Dielectrophoretic Effect of Fluorescent Dyes at Single-Cell Resolution
title_full Quantitative Investigation for the Dielectrophoretic Effect of Fluorescent Dyes at Single-Cell Resolution
title_fullStr Quantitative Investigation for the Dielectrophoretic Effect of Fluorescent Dyes at Single-Cell Resolution
title_full_unstemmed Quantitative Investigation for the Dielectrophoretic Effect of Fluorescent Dyes at Single-Cell Resolution
title_short Quantitative Investigation for the Dielectrophoretic Effect of Fluorescent Dyes at Single-Cell Resolution
title_sort quantitative investigation for the dielectrophoretic effect of fluorescent dyes at single-cell resolution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068594/
https://www.ncbi.nlm.nih.gov/pubmed/30087910
http://dx.doi.org/10.1021/acsomega.8b00541
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