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An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests

This paper proposes a new yet efficient method allowing a significant improvement in the on-line analysis of biological cell growing and evolution. The procedure is based on an empirical-mathematical approach for calibration and fitting of any cell-electrode electrical model. It is valid and can be...

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Autores principales: Serrano, Juan A., Huertas, Gloria, Maldonado-Jacobi, Andrés, Olmo, Alberto, Pérez, Pablo, Martín, María E., Daza, Paula, Yúfera, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068773/
https://www.ncbi.nlm.nih.gov/pubmed/30036948
http://dx.doi.org/10.3390/s18072354
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author Serrano, Juan A.
Huertas, Gloria
Maldonado-Jacobi, Andrés
Olmo, Alberto
Pérez, Pablo
Martín, María E.
Daza, Paula
Yúfera, Alberto
author_facet Serrano, Juan A.
Huertas, Gloria
Maldonado-Jacobi, Andrés
Olmo, Alberto
Pérez, Pablo
Martín, María E.
Daza, Paula
Yúfera, Alberto
author_sort Serrano, Juan A.
collection PubMed
description This paper proposes a new yet efficient method allowing a significant improvement in the on-line analysis of biological cell growing and evolution. The procedure is based on an empirical-mathematical approach for calibration and fitting of any cell-electrode electrical model. It is valid and can be extrapolated for any type of cellular line used in electrical cell-substrate impedance spectroscopy (ECIS) tests. Parameters of the bioimpedance model, acquired from ECIS experiments, vary for each cell line, which makes obtaining results difficult and—to some extent-renders them inaccurate. We propose a fitting method based on the cell line initial characterization, and carry out subsequent experiments with the same line to approach the percentage of well filling and the cell density (or cell number in the well). To perform our calibration technique, the so-called oscillation-based test (OBT) approach is employed for each cell density. Calibration results are validated by performing other experiments with different concentrations on the same cell line with the same measurement technique. Accordingly, a bioimpedance electrical model of each cell line is determined, which is valid for any further experiment and leading to a more precise electrical model of the electrode-cell system. Furthermore, the model parameters calculated can be also used by any other measurement techniques. Promising experimental outcomes for three different cell-lines have been achieved, supporting the usefulness of this technique.
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spelling pubmed-60687732018-08-07 An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests Serrano, Juan A. Huertas, Gloria Maldonado-Jacobi, Andrés Olmo, Alberto Pérez, Pablo Martín, María E. Daza, Paula Yúfera, Alberto Sensors (Basel) Article This paper proposes a new yet efficient method allowing a significant improvement in the on-line analysis of biological cell growing and evolution. The procedure is based on an empirical-mathematical approach for calibration and fitting of any cell-electrode electrical model. It is valid and can be extrapolated for any type of cellular line used in electrical cell-substrate impedance spectroscopy (ECIS) tests. Parameters of the bioimpedance model, acquired from ECIS experiments, vary for each cell line, which makes obtaining results difficult and—to some extent-renders them inaccurate. We propose a fitting method based on the cell line initial characterization, and carry out subsequent experiments with the same line to approach the percentage of well filling and the cell density (or cell number in the well). To perform our calibration technique, the so-called oscillation-based test (OBT) approach is employed for each cell density. Calibration results are validated by performing other experiments with different concentrations on the same cell line with the same measurement technique. Accordingly, a bioimpedance electrical model of each cell line is determined, which is valid for any further experiment and leading to a more precise electrical model of the electrode-cell system. Furthermore, the model parameters calculated can be also used by any other measurement techniques. Promising experimental outcomes for three different cell-lines have been achieved, supporting the usefulness of this technique. MDPI 2018-07-20 /pmc/articles/PMC6068773/ /pubmed/30036948 http://dx.doi.org/10.3390/s18072354 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Serrano, Juan A.
Huertas, Gloria
Maldonado-Jacobi, Andrés
Olmo, Alberto
Pérez, Pablo
Martín, María E.
Daza, Paula
Yúfera, Alberto
An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests
title An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests
title_full An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests
title_fullStr An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests
title_full_unstemmed An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests
title_short An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests
title_sort empirical-mathematical approach for calibration and fitting cell-electrode electrical models in bioimpedance tests
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068773/
https://www.ncbi.nlm.nih.gov/pubmed/30036948
http://dx.doi.org/10.3390/s18072354
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