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DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos

In this work, we propose an electrochemical DNA aptasensor for the detection of profenofos, an organophosphorus pesticide, based on a competitive format and disposable graphite screen-printed electrodes (GSPEs). A thiol-tethered DNA capture probe, which results to be complementary to the chosen apta...

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Detalles Bibliográficos
Autores principales: Selvolini, Giulia, Băjan, Ioana, Hosu, Oana, Cristea, Cecilia, Săndulescu, Robert, Marrazza, Giovanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068880/
https://www.ncbi.nlm.nih.gov/pubmed/29941847
http://dx.doi.org/10.3390/s18072035
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author Selvolini, Giulia
Băjan, Ioana
Hosu, Oana
Cristea, Cecilia
Săndulescu, Robert
Marrazza, Giovanna
author_facet Selvolini, Giulia
Băjan, Ioana
Hosu, Oana
Cristea, Cecilia
Săndulescu, Robert
Marrazza, Giovanna
author_sort Selvolini, Giulia
collection PubMed
description In this work, we propose an electrochemical DNA aptasensor for the detection of profenofos, an organophosphorus pesticide, based on a competitive format and disposable graphite screen-printed electrodes (GSPEs). A thiol-tethered DNA capture probe, which results to be complementary to the chosen aptamer sequence, was immobilised on gold nanoparticles/polyaniline composite film-modified electrodes (AuNPs/PANI/GSPE). Different profenofos solutions containing a fixed amount of the biotinylated DNA aptamer were dropped onto the realized aptasensors. The hybridisation reaction was measured using a streptavidin-alkaline phosphatase enzyme conjugate, which catalyses the hydrolysis of 1-naphthyl -phosphate. The 1-naphtol enzymatic product was detected by means of differential pulse voltammetry (DPV). The aptasensor showed itself to work as a signal off sensor, according to the competitive format used. A dose response curve was obtained between 0.10 μM and 10 μM with a detection limit of 0.27 μM.
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spelling pubmed-60688802018-08-07 DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos Selvolini, Giulia Băjan, Ioana Hosu, Oana Cristea, Cecilia Săndulescu, Robert Marrazza, Giovanna Sensors (Basel) Article In this work, we propose an electrochemical DNA aptasensor for the detection of profenofos, an organophosphorus pesticide, based on a competitive format and disposable graphite screen-printed electrodes (GSPEs). A thiol-tethered DNA capture probe, which results to be complementary to the chosen aptamer sequence, was immobilised on gold nanoparticles/polyaniline composite film-modified electrodes (AuNPs/PANI/GSPE). Different profenofos solutions containing a fixed amount of the biotinylated DNA aptamer were dropped onto the realized aptasensors. The hybridisation reaction was measured using a streptavidin-alkaline phosphatase enzyme conjugate, which catalyses the hydrolysis of 1-naphthyl -phosphate. The 1-naphtol enzymatic product was detected by means of differential pulse voltammetry (DPV). The aptasensor showed itself to work as a signal off sensor, according to the competitive format used. A dose response curve was obtained between 0.10 μM and 10 μM with a detection limit of 0.27 μM. MDPI 2018-06-25 /pmc/articles/PMC6068880/ /pubmed/29941847 http://dx.doi.org/10.3390/s18072035 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Selvolini, Giulia
Băjan, Ioana
Hosu, Oana
Cristea, Cecilia
Săndulescu, Robert
Marrazza, Giovanna
DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos
title DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos
title_full DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos
title_fullStr DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos
title_full_unstemmed DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos
title_short DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos
title_sort dna-based sensor for the detection of an organophosphorus pesticide: profenofos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068880/
https://www.ncbi.nlm.nih.gov/pubmed/29941847
http://dx.doi.org/10.3390/s18072035
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