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DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos
In this work, we propose an electrochemical DNA aptasensor for the detection of profenofos, an organophosphorus pesticide, based on a competitive format and disposable graphite screen-printed electrodes (GSPEs). A thiol-tethered DNA capture probe, which results to be complementary to the chosen apta...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068880/ https://www.ncbi.nlm.nih.gov/pubmed/29941847 http://dx.doi.org/10.3390/s18072035 |
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author | Selvolini, Giulia Băjan, Ioana Hosu, Oana Cristea, Cecilia Săndulescu, Robert Marrazza, Giovanna |
author_facet | Selvolini, Giulia Băjan, Ioana Hosu, Oana Cristea, Cecilia Săndulescu, Robert Marrazza, Giovanna |
author_sort | Selvolini, Giulia |
collection | PubMed |
description | In this work, we propose an electrochemical DNA aptasensor for the detection of profenofos, an organophosphorus pesticide, based on a competitive format and disposable graphite screen-printed electrodes (GSPEs). A thiol-tethered DNA capture probe, which results to be complementary to the chosen aptamer sequence, was immobilised on gold nanoparticles/polyaniline composite film-modified electrodes (AuNPs/PANI/GSPE). Different profenofos solutions containing a fixed amount of the biotinylated DNA aptamer were dropped onto the realized aptasensors. The hybridisation reaction was measured using a streptavidin-alkaline phosphatase enzyme conjugate, which catalyses the hydrolysis of 1-naphthyl -phosphate. The 1-naphtol enzymatic product was detected by means of differential pulse voltammetry (DPV). The aptasensor showed itself to work as a signal off sensor, according to the competitive format used. A dose response curve was obtained between 0.10 μM and 10 μM with a detection limit of 0.27 μM. |
format | Online Article Text |
id | pubmed-6068880 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60688802018-08-07 DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos Selvolini, Giulia Băjan, Ioana Hosu, Oana Cristea, Cecilia Săndulescu, Robert Marrazza, Giovanna Sensors (Basel) Article In this work, we propose an electrochemical DNA aptasensor for the detection of profenofos, an organophosphorus pesticide, based on a competitive format and disposable graphite screen-printed electrodes (GSPEs). A thiol-tethered DNA capture probe, which results to be complementary to the chosen aptamer sequence, was immobilised on gold nanoparticles/polyaniline composite film-modified electrodes (AuNPs/PANI/GSPE). Different profenofos solutions containing a fixed amount of the biotinylated DNA aptamer were dropped onto the realized aptasensors. The hybridisation reaction was measured using a streptavidin-alkaline phosphatase enzyme conjugate, which catalyses the hydrolysis of 1-naphthyl -phosphate. The 1-naphtol enzymatic product was detected by means of differential pulse voltammetry (DPV). The aptasensor showed itself to work as a signal off sensor, according to the competitive format used. A dose response curve was obtained between 0.10 μM and 10 μM with a detection limit of 0.27 μM. MDPI 2018-06-25 /pmc/articles/PMC6068880/ /pubmed/29941847 http://dx.doi.org/10.3390/s18072035 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Selvolini, Giulia Băjan, Ioana Hosu, Oana Cristea, Cecilia Săndulescu, Robert Marrazza, Giovanna DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos |
title | DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos |
title_full | DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos |
title_fullStr | DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos |
title_full_unstemmed | DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos |
title_short | DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos |
title_sort | dna-based sensor for the detection of an organophosphorus pesticide: profenofos |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068880/ https://www.ncbi.nlm.nih.gov/pubmed/29941847 http://dx.doi.org/10.3390/s18072035 |
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