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Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10

Background: In this study, the effectiveness of dexpanthenol and coenzyme Q10 (CoQ10) on the healing of ischemic colon anastomosis was investigated. Methods: Forty eight male Wistar Albino rats were divided into four equal groups (Sham-S, Sham-I, DXP, Q10). Following full layer colon resection, sing...

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Autores principales: Pehlivanlı, Faruk, Aydin, Oktay, Karaca, Gökhan, Aydin, Gülçin, Devrim, Tuba, Bulut, Huri, Bakar, Bülent, Daphan, Çağatay Erden
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6069384/
https://www.ncbi.nlm.nih.gov/pubmed/29941836
http://dx.doi.org/10.3390/jcm7070161
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author Pehlivanlı, Faruk
Aydin, Oktay
Karaca, Gökhan
Aydin, Gülçin
Devrim, Tuba
Bulut, Huri
Bakar, Bülent
Daphan, Çağatay Erden
author_facet Pehlivanlı, Faruk
Aydin, Oktay
Karaca, Gökhan
Aydin, Gülçin
Devrim, Tuba
Bulut, Huri
Bakar, Bülent
Daphan, Çağatay Erden
author_sort Pehlivanlı, Faruk
collection PubMed
description Background: In this study, the effectiveness of dexpanthenol and coenzyme Q10 (CoQ10) on the healing of ischemic colon anastomosis was investigated. Methods: Forty eight male Wistar Albino rats were divided into four equal groups (Sham-S, Sham-I, DXP, Q10). Following full layer colon resection, single layer colon anastomosis, without creating ischemia, was performed on the Sham-S group. The same experimental model was performed on remaining groups after ischemia was created. Intraperitoneal dexpanthenol and CoQ10 was administered to the DXP and Q10 groups once a day for three days. Ten days later, all colon anastomoses were investigated histopathologically and biochemically, as well as their burst pressure values, in all sacrificed rats. Results: The highest burst pressure value was observed in the Sham-S group, decreasing from high to low in the DXP, Q10, and Sham-I groups, respectively (p = 0.008). Furthermore, tissue hydroxyproline (p = 0.001) level values were significantly different among the groups. Additionally, histopathological analysis revealed a significant difference among groups regarding reepithelization (p = 0.027) and polymorphonuclear leukocyte density (p = 0.022). Conclusions: This preliminary study has shown that ischemia-reperfusion injury may impair the healing of colon anastomosis and it has been concluded that dexpanthenol and CoQ10 may have positive effects on the healing of ischemic colon anastomosis in rat, although re-epithelization may be adversely affected using CoQ10.
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spelling pubmed-60693842018-08-07 Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10 Pehlivanlı, Faruk Aydin, Oktay Karaca, Gökhan Aydin, Gülçin Devrim, Tuba Bulut, Huri Bakar, Bülent Daphan, Çağatay Erden J Clin Med Article Background: In this study, the effectiveness of dexpanthenol and coenzyme Q10 (CoQ10) on the healing of ischemic colon anastomosis was investigated. Methods: Forty eight male Wistar Albino rats were divided into four equal groups (Sham-S, Sham-I, DXP, Q10). Following full layer colon resection, single layer colon anastomosis, without creating ischemia, was performed on the Sham-S group. The same experimental model was performed on remaining groups after ischemia was created. Intraperitoneal dexpanthenol and CoQ10 was administered to the DXP and Q10 groups once a day for three days. Ten days later, all colon anastomoses were investigated histopathologically and biochemically, as well as their burst pressure values, in all sacrificed rats. Results: The highest burst pressure value was observed in the Sham-S group, decreasing from high to low in the DXP, Q10, and Sham-I groups, respectively (p = 0.008). Furthermore, tissue hydroxyproline (p = 0.001) level values were significantly different among the groups. Additionally, histopathological analysis revealed a significant difference among groups regarding reepithelization (p = 0.027) and polymorphonuclear leukocyte density (p = 0.022). Conclusions: This preliminary study has shown that ischemia-reperfusion injury may impair the healing of colon anastomosis and it has been concluded that dexpanthenol and CoQ10 may have positive effects on the healing of ischemic colon anastomosis in rat, although re-epithelization may be adversely affected using CoQ10. MDPI 2018-06-25 /pmc/articles/PMC6069384/ /pubmed/29941836 http://dx.doi.org/10.3390/jcm7070161 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pehlivanlı, Faruk
Aydin, Oktay
Karaca, Gökhan
Aydin, Gülçin
Devrim, Tuba
Bulut, Huri
Bakar, Bülent
Daphan, Çağatay Erden
Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10
title Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10
title_full Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10
title_fullStr Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10
title_full_unstemmed Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10
title_short Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10
title_sort healing of ischemic colon anastomosis in rats could be provided by administering dexpanthenol or coenzyme q10
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6069384/
https://www.ncbi.nlm.nih.gov/pubmed/29941836
http://dx.doi.org/10.3390/jcm7070161
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