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Two-Step Generation of Oligodendrocyte Progenitor Cells From Mouse Fibroblasts for Spinal Cord Injury
Oligodendrocyte progenitor cells (OPCs) are attracting attention as the ideal cell therapy for spinal cord injury (SCI). Recently, advanced reprogramming and differentiation techniques have made it possible to generate therapeutic cells for treating SCI. In the present study, we used directly-induce...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070016/ https://www.ncbi.nlm.nih.gov/pubmed/30090058 http://dx.doi.org/10.3389/fncel.2018.00198 |
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author | Lee, Yukyeong Kim, C-Yoon Lee, Hye Jeong Kim, Jae Gon Han, Dong Wook Ko, Kisung Walter, James Chung, Hyung-Min Schöler, Hans R. Bae, Young Min Ko, Kinarm |
author_facet | Lee, Yukyeong Kim, C-Yoon Lee, Hye Jeong Kim, Jae Gon Han, Dong Wook Ko, Kisung Walter, James Chung, Hyung-Min Schöler, Hans R. Bae, Young Min Ko, Kinarm |
author_sort | Lee, Yukyeong |
collection | PubMed |
description | Oligodendrocyte progenitor cells (OPCs) are attracting attention as the ideal cell therapy for spinal cord injury (SCI). Recently, advanced reprogramming and differentiation techniques have made it possible to generate therapeutic cells for treating SCI. In the present study, we used directly-induced neural stem cells (DNSCs) from fibroblasts to establish OPCs (DN-OPCs) capable of proliferation and confirmed their OPC-specific characteristics. Also, we evaluated the effect of transplanted DN-OPCs on SCI in rats. The DN-OPCs exhibited an OPC-specific phenotype and electrophysiological function and could be differentiated into oligodendrocytes. In the SCI model, transplanted DN-OPCs improved behavior recovery, and showed engraftment into the host spinal cord with expression of myelin basic protein. These results suggest that DN-OPCs could be a new source of potentially useful cells for treating SCI. |
format | Online Article Text |
id | pubmed-6070016 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60700162018-08-08 Two-Step Generation of Oligodendrocyte Progenitor Cells From Mouse Fibroblasts for Spinal Cord Injury Lee, Yukyeong Kim, C-Yoon Lee, Hye Jeong Kim, Jae Gon Han, Dong Wook Ko, Kisung Walter, James Chung, Hyung-Min Schöler, Hans R. Bae, Young Min Ko, Kinarm Front Cell Neurosci Neuroscience Oligodendrocyte progenitor cells (OPCs) are attracting attention as the ideal cell therapy for spinal cord injury (SCI). Recently, advanced reprogramming and differentiation techniques have made it possible to generate therapeutic cells for treating SCI. In the present study, we used directly-induced neural stem cells (DNSCs) from fibroblasts to establish OPCs (DN-OPCs) capable of proliferation and confirmed their OPC-specific characteristics. Also, we evaluated the effect of transplanted DN-OPCs on SCI in rats. The DN-OPCs exhibited an OPC-specific phenotype and electrophysiological function and could be differentiated into oligodendrocytes. In the SCI model, transplanted DN-OPCs improved behavior recovery, and showed engraftment into the host spinal cord with expression of myelin basic protein. These results suggest that DN-OPCs could be a new source of potentially useful cells for treating SCI. Frontiers Media S.A. 2018-07-25 /pmc/articles/PMC6070016/ /pubmed/30090058 http://dx.doi.org/10.3389/fncel.2018.00198 Text en Copyright © 2018 Lee, Kim, Lee, Kim, Han, Ko, Walter, Chung, Schöler, Bae and Ko. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Lee, Yukyeong Kim, C-Yoon Lee, Hye Jeong Kim, Jae Gon Han, Dong Wook Ko, Kisung Walter, James Chung, Hyung-Min Schöler, Hans R. Bae, Young Min Ko, Kinarm Two-Step Generation of Oligodendrocyte Progenitor Cells From Mouse Fibroblasts for Spinal Cord Injury |
title | Two-Step Generation of Oligodendrocyte Progenitor Cells From Mouse Fibroblasts for Spinal Cord Injury |
title_full | Two-Step Generation of Oligodendrocyte Progenitor Cells From Mouse Fibroblasts for Spinal Cord Injury |
title_fullStr | Two-Step Generation of Oligodendrocyte Progenitor Cells From Mouse Fibroblasts for Spinal Cord Injury |
title_full_unstemmed | Two-Step Generation of Oligodendrocyte Progenitor Cells From Mouse Fibroblasts for Spinal Cord Injury |
title_short | Two-Step Generation of Oligodendrocyte Progenitor Cells From Mouse Fibroblasts for Spinal Cord Injury |
title_sort | two-step generation of oligodendrocyte progenitor cells from mouse fibroblasts for spinal cord injury |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070016/ https://www.ncbi.nlm.nih.gov/pubmed/30090058 http://dx.doi.org/10.3389/fncel.2018.00198 |
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