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Comparative transcriptomics reveals specific responding genes associated with atherosclerosis in rabbit and mouse models
Mouse and rabbit are frequently employed species for atherosclerosis research. With respect to modeling human atherosclerosis, it has been observed that variations in phenotype under commonly used atherogenic conditions are partial or no congruence between two species. However, genome-wide molecular...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070260/ https://www.ncbi.nlm.nih.gov/pubmed/30067832 http://dx.doi.org/10.1371/journal.pone.0201618 |
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author | Wu, Leilei Yao, Qianlan Lin, Ping Li, Yixue Li, Hong |
author_facet | Wu, Leilei Yao, Qianlan Lin, Ping Li, Yixue Li, Hong |
author_sort | Wu, Leilei |
collection | PubMed |
description | Mouse and rabbit are frequently employed species for atherosclerosis research. With respect to modeling human atherosclerosis, it has been observed that variations in phenotype under commonly used atherogenic conditions are partial or no congruence between two species. However, genome-wide molecular variations are still lacking. To understand the differences between rabbit and mouse in developing atherosclerosis, here from aspect of orthologs, we compared the genome-wide expression profiles of two species under the same atherosclerosis driven factors: high-fat diet or LDLR deficiency. Our results illuminated that: 1) LDLR-deficiency induced different gene expression changes in rabbit and mouse. WHHL rabbit had more significantly differential expressed genes and the most of genes were down-regulated. 2) Some genes and functions were commonly dysregulated in high-fat fed rabbit and mouse models, such as lipid metabolism and inflammation process. However, high-fat intake in rabbit produced more differentially expressed genes and more serious functional effects. 3) Specific differential expression genes were revealed for rabbit and mouse related with high-fat intake. In the aspect of lipoprotein metabolism, APOA4 and APOB was the major responding gene in rabbit and mice, respectively. The expression change of APOA4 and APOB in human atherosclerosis was more similar to rabbit, and therefore rabbit might be a better animal model for investigating human lipoprotein metabolism related diseases. In conclusion, our comparative transcriptome analysis revealed species-specific expression regulation that could partially explain the different phenotypes between rabbit and mouse, which was helpful for model selection to study atherosclerosis. |
format | Online Article Text |
id | pubmed-6070260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60702602018-08-09 Comparative transcriptomics reveals specific responding genes associated with atherosclerosis in rabbit and mouse models Wu, Leilei Yao, Qianlan Lin, Ping Li, Yixue Li, Hong PLoS One Research Article Mouse and rabbit are frequently employed species for atherosclerosis research. With respect to modeling human atherosclerosis, it has been observed that variations in phenotype under commonly used atherogenic conditions are partial or no congruence between two species. However, genome-wide molecular variations are still lacking. To understand the differences between rabbit and mouse in developing atherosclerosis, here from aspect of orthologs, we compared the genome-wide expression profiles of two species under the same atherosclerosis driven factors: high-fat diet or LDLR deficiency. Our results illuminated that: 1) LDLR-deficiency induced different gene expression changes in rabbit and mouse. WHHL rabbit had more significantly differential expressed genes and the most of genes were down-regulated. 2) Some genes and functions were commonly dysregulated in high-fat fed rabbit and mouse models, such as lipid metabolism and inflammation process. However, high-fat intake in rabbit produced more differentially expressed genes and more serious functional effects. 3) Specific differential expression genes were revealed for rabbit and mouse related with high-fat intake. In the aspect of lipoprotein metabolism, APOA4 and APOB was the major responding gene in rabbit and mice, respectively. The expression change of APOA4 and APOB in human atherosclerosis was more similar to rabbit, and therefore rabbit might be a better animal model for investigating human lipoprotein metabolism related diseases. In conclusion, our comparative transcriptome analysis revealed species-specific expression regulation that could partially explain the different phenotypes between rabbit and mouse, which was helpful for model selection to study atherosclerosis. Public Library of Science 2018-08-01 /pmc/articles/PMC6070260/ /pubmed/30067832 http://dx.doi.org/10.1371/journal.pone.0201618 Text en © 2018 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wu, Leilei Yao, Qianlan Lin, Ping Li, Yixue Li, Hong Comparative transcriptomics reveals specific responding genes associated with atherosclerosis in rabbit and mouse models |
title | Comparative transcriptomics reveals specific responding genes associated with atherosclerosis in rabbit and mouse models |
title_full | Comparative transcriptomics reveals specific responding genes associated with atherosclerosis in rabbit and mouse models |
title_fullStr | Comparative transcriptomics reveals specific responding genes associated with atherosclerosis in rabbit and mouse models |
title_full_unstemmed | Comparative transcriptomics reveals specific responding genes associated with atherosclerosis in rabbit and mouse models |
title_short | Comparative transcriptomics reveals specific responding genes associated with atherosclerosis in rabbit and mouse models |
title_sort | comparative transcriptomics reveals specific responding genes associated with atherosclerosis in rabbit and mouse models |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070260/ https://www.ncbi.nlm.nih.gov/pubmed/30067832 http://dx.doi.org/10.1371/journal.pone.0201618 |
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