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BioBits™ Bright: A fluorescent synthetic biology education kit
Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow li...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070313/ https://www.ncbi.nlm.nih.gov/pubmed/30083609 http://dx.doi.org/10.1126/sciadv.aat5107 |
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author | Stark, Jessica C. Huang, Ally Nguyen, Peter Q. Dubner, Rachel S. Hsu, Karen J. Ferrante, Thomas C. Anderson, Mary Kanapskyte, Ada Mucha, Quinn Packett, Jessica S. Patel, Palak Patel, Richa Qaq, Deema Zondor, Tyler Burke, Julie Martinez, Thomas Miller-Berry, Ashlee Puppala, Aparna Reichert, Kara Schmid, Miriam Brand, Lance Hill, Lander R. Chellaswamy, Jemima F. Faheem, Nuhie Fetherling, Suzanne Gong, Elissa Gonzalzles, Eddie Marie Granito, Teresa Koritsaris, Jenna Nguyen, Binh Ottman, Sujud Palffy, Christina Patel, Angela Skweres, Sheila Slaton, Adriane Woods, TaRhonda Donghia, Nina Pardee, Keith Collins, James J. Jewett, Michael C. |
author_facet | Stark, Jessica C. Huang, Ally Nguyen, Peter Q. Dubner, Rachel S. Hsu, Karen J. Ferrante, Thomas C. Anderson, Mary Kanapskyte, Ada Mucha, Quinn Packett, Jessica S. Patel, Palak Patel, Richa Qaq, Deema Zondor, Tyler Burke, Julie Martinez, Thomas Miller-Berry, Ashlee Puppala, Aparna Reichert, Kara Schmid, Miriam Brand, Lance Hill, Lander R. Chellaswamy, Jemima F. Faheem, Nuhie Fetherling, Suzanne Gong, Elissa Gonzalzles, Eddie Marie Granito, Teresa Koritsaris, Jenna Nguyen, Binh Ottman, Sujud Palffy, Christina Patel, Angela Skweres, Sheila Slaton, Adriane Woods, TaRhonda Donghia, Nina Pardee, Keith Collins, James J. Jewett, Michael C. |
author_sort | Stark, Jessica C. |
collection | PubMed |
description | Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow living cells. We present BioBits™ Bright, a shelf-stable, just-add-water synthetic biology education kit with easy visual outputs enabled by expression of fluorescent proteins in freeze-dried, cell-free reactions. We introduce activities and supporting curricula for teaching the central dogma, tunable protein expression, and design-build-test cycles and report data generated by K-12 teachers and students. We also develop inexpensive incubators and imagers, resulting in a comprehensive kit costing <US$100 per 30-person classroom. The user-friendly resources of this kit promise to enhance biology education both inside and outside the classroom. |
format | Online Article Text |
id | pubmed-6070313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60703132018-08-06 BioBits™ Bright: A fluorescent synthetic biology education kit Stark, Jessica C. Huang, Ally Nguyen, Peter Q. Dubner, Rachel S. Hsu, Karen J. Ferrante, Thomas C. Anderson, Mary Kanapskyte, Ada Mucha, Quinn Packett, Jessica S. Patel, Palak Patel, Richa Qaq, Deema Zondor, Tyler Burke, Julie Martinez, Thomas Miller-Berry, Ashlee Puppala, Aparna Reichert, Kara Schmid, Miriam Brand, Lance Hill, Lander R. Chellaswamy, Jemima F. Faheem, Nuhie Fetherling, Suzanne Gong, Elissa Gonzalzles, Eddie Marie Granito, Teresa Koritsaris, Jenna Nguyen, Binh Ottman, Sujud Palffy, Christina Patel, Angela Skweres, Sheila Slaton, Adriane Woods, TaRhonda Donghia, Nina Pardee, Keith Collins, James J. Jewett, Michael C. Sci Adv Research Articles Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow living cells. We present BioBits™ Bright, a shelf-stable, just-add-water synthetic biology education kit with easy visual outputs enabled by expression of fluorescent proteins in freeze-dried, cell-free reactions. We introduce activities and supporting curricula for teaching the central dogma, tunable protein expression, and design-build-test cycles and report data generated by K-12 teachers and students. We also develop inexpensive incubators and imagers, resulting in a comprehensive kit costing <US$100 per 30-person classroom. The user-friendly resources of this kit promise to enhance biology education both inside and outside the classroom. American Association for the Advancement of Science 2018-08-01 /pmc/articles/PMC6070313/ /pubmed/30083609 http://dx.doi.org/10.1126/sciadv.aat5107 Text en Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Stark, Jessica C. Huang, Ally Nguyen, Peter Q. Dubner, Rachel S. Hsu, Karen J. Ferrante, Thomas C. Anderson, Mary Kanapskyte, Ada Mucha, Quinn Packett, Jessica S. Patel, Palak Patel, Richa Qaq, Deema Zondor, Tyler Burke, Julie Martinez, Thomas Miller-Berry, Ashlee Puppala, Aparna Reichert, Kara Schmid, Miriam Brand, Lance Hill, Lander R. Chellaswamy, Jemima F. Faheem, Nuhie Fetherling, Suzanne Gong, Elissa Gonzalzles, Eddie Marie Granito, Teresa Koritsaris, Jenna Nguyen, Binh Ottman, Sujud Palffy, Christina Patel, Angela Skweres, Sheila Slaton, Adriane Woods, TaRhonda Donghia, Nina Pardee, Keith Collins, James J. Jewett, Michael C. BioBits™ Bright: A fluorescent synthetic biology education kit |
title | BioBits™ Bright: A fluorescent synthetic biology education kit |
title_full | BioBits™ Bright: A fluorescent synthetic biology education kit |
title_fullStr | BioBits™ Bright: A fluorescent synthetic biology education kit |
title_full_unstemmed | BioBits™ Bright: A fluorescent synthetic biology education kit |
title_short | BioBits™ Bright: A fluorescent synthetic biology education kit |
title_sort | biobits™ bright: a fluorescent synthetic biology education kit |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070313/ https://www.ncbi.nlm.nih.gov/pubmed/30083609 http://dx.doi.org/10.1126/sciadv.aat5107 |
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