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Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus

In this study, we provide the first description of Cystoisospora infection in Asian small-clawed otters (Aonyx cinereus). In July 2017, three juvenile otters recently imported from the Republic of Indonesia showed severe diarrhea and were diagnosed with coccidial infection; two of them eventually di...

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Autores principales: Tokiwa, Toshihiro, Ohnuki, Ai, Kubota, Rie, Tamukai, Kenichi, Ike, Kazunori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070675/
https://www.ncbi.nlm.nih.gov/pubmed/30094175
http://dx.doi.org/10.1016/j.ijppaw.2018.07.001
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author Tokiwa, Toshihiro
Ohnuki, Ai
Kubota, Rie
Tamukai, Kenichi
Ike, Kazunori
author_facet Tokiwa, Toshihiro
Ohnuki, Ai
Kubota, Rie
Tamukai, Kenichi
Ike, Kazunori
author_sort Tokiwa, Toshihiro
collection PubMed
description In this study, we provide the first description of Cystoisospora infection in Asian small-clawed otters (Aonyx cinereus). In July 2017, three juvenile otters recently imported from the Republic of Indonesia showed severe diarrhea and were diagnosed with coccidial infection; two of them eventually died. Fecal examination revealed the presence of numerous oocysts. Sporulated oocysts showed typical Cystoisospora features, measuring 24.6 ± 1.6 (22.0–27.0) × 21.8 ± 1.4 (19.0–25.0) μm, with an oocyst length/width ratio of 1.1 ± 0.1 (1.0–1.3). Each sporocyst contained four sporozoites in a head-to-tail arrangement. The Stieda body was absent, and the sporocyst residuum was present. These morphological characteristics differentiated this species from the other valid Cystoisospora species described from mustelids. Molecular analysis was conducted at two loci: the nuclear 18S ribosomal RNA and mitochondrial cytochrome c oxidase subunit I genes. The 18S sequence showed high similarity with canine Cystoisispora ohioensis (1-bp difference, 1422/1423 [99.9%]). At the cytochrome c oxidase subunit I gene locus, the sequence from otters was identical to that of feline Cystoisospora rivolta (847/847 [100%]). Phylogenetic analyses using concatenated data demonstrated that Cystoisospora sp. from otters and C. rivolta grouped together in the same Cystoisospora clade. Based on these data, we concluded that Cystoisospora sp. detected from otters appeared to be highly similar to C. rivolta.
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spelling pubmed-60706752018-08-09 Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus Tokiwa, Toshihiro Ohnuki, Ai Kubota, Rie Tamukai, Kenichi Ike, Kazunori Int J Parasitol Parasites Wildl Article In this study, we provide the first description of Cystoisospora infection in Asian small-clawed otters (Aonyx cinereus). In July 2017, three juvenile otters recently imported from the Republic of Indonesia showed severe diarrhea and were diagnosed with coccidial infection; two of them eventually died. Fecal examination revealed the presence of numerous oocysts. Sporulated oocysts showed typical Cystoisospora features, measuring 24.6 ± 1.6 (22.0–27.0) × 21.8 ± 1.4 (19.0–25.0) μm, with an oocyst length/width ratio of 1.1 ± 0.1 (1.0–1.3). Each sporocyst contained four sporozoites in a head-to-tail arrangement. The Stieda body was absent, and the sporocyst residuum was present. These morphological characteristics differentiated this species from the other valid Cystoisospora species described from mustelids. Molecular analysis was conducted at two loci: the nuclear 18S ribosomal RNA and mitochondrial cytochrome c oxidase subunit I genes. The 18S sequence showed high similarity with canine Cystoisispora ohioensis (1-bp difference, 1422/1423 [99.9%]). At the cytochrome c oxidase subunit I gene locus, the sequence from otters was identical to that of feline Cystoisospora rivolta (847/847 [100%]). Phylogenetic analyses using concatenated data demonstrated that Cystoisospora sp. from otters and C. rivolta grouped together in the same Cystoisospora clade. Based on these data, we concluded that Cystoisospora sp. detected from otters appeared to be highly similar to C. rivolta. Elsevier 2018-07-11 /pmc/articles/PMC6070675/ /pubmed/30094175 http://dx.doi.org/10.1016/j.ijppaw.2018.07.001 Text en © 2018 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Tokiwa, Toshihiro
Ohnuki, Ai
Kubota, Rie
Tamukai, Kenichi
Ike, Kazunori
Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus
title Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus
title_full Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus
title_fullStr Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus
title_full_unstemmed Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus
title_short Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus
title_sort morphological and molecular characterization of cystoisospora sp. from asian small-clawed otters aonyx cinereus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070675/
https://www.ncbi.nlm.nih.gov/pubmed/30094175
http://dx.doi.org/10.1016/j.ijppaw.2018.07.001
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