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miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation
Human pluripotent stem cells retain the extraordinary capacity to differentiate into pancreatic beta cells. For this particular lineage, more effort is still required to stress the importance of developing an efficient, reproducible, easy, and cost-effective differentiation protocol to obtain more m...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070677/ https://www.ncbi.nlm.nih.gov/pubmed/30195784 http://dx.doi.org/10.1016/j.omtn.2018.06.002 |
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author | López-Beas, Javier Capilla-González, Vivian Aguilera, Yolanda Mellado, Nuria Lachaud, Christian C. Martín, Franz Smani, Tarik Soria, Bernat Hmadcha, Abdelkrim |
author_facet | López-Beas, Javier Capilla-González, Vivian Aguilera, Yolanda Mellado, Nuria Lachaud, Christian C. Martín, Franz Smani, Tarik Soria, Bernat Hmadcha, Abdelkrim |
author_sort | López-Beas, Javier |
collection | PubMed |
description | Human pluripotent stem cells retain the extraordinary capacity to differentiate into pancreatic beta cells. For this particular lineage, more effort is still required to stress the importance of developing an efficient, reproducible, easy, and cost-effective differentiation protocol to obtain more mature, homogeneous, and functional insulin-secreting cells. In addition, microRNAs (miRNAs) have emerged as a class of small non-coding RNAs that regulate many cellular processes, including pancreatic differentiation. Some miRNAs are known to be preferentially expressed in islets. Of note, miR-375 and miR-7 are two of the most abundant pancreatic miRNAs, and they are necessary for proper pancreatic islet development. Here we provide new insight into specific miRNAs involved in pancreatic differentiation. We found that miR-7 is differentially expressed during the differentiation of human embryonic stem cells (hESCs) into a beta cell-like phenotype and that its modulation plays an important role in generating mature pancreatic beta cells. This strategy may be exploited to optimize the potential for in vitro differentiation of hESCs into insulin-producing beta-like cells for use in preclinical studies and future clinical applications as well as the prospective uses of miRNAs to improve this process. |
format | Online Article Text |
id | pubmed-6070677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-60706772018-08-02 miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation López-Beas, Javier Capilla-González, Vivian Aguilera, Yolanda Mellado, Nuria Lachaud, Christian C. Martín, Franz Smani, Tarik Soria, Bernat Hmadcha, Abdelkrim Mol Ther Nucleic Acids Article Human pluripotent stem cells retain the extraordinary capacity to differentiate into pancreatic beta cells. For this particular lineage, more effort is still required to stress the importance of developing an efficient, reproducible, easy, and cost-effective differentiation protocol to obtain more mature, homogeneous, and functional insulin-secreting cells. In addition, microRNAs (miRNAs) have emerged as a class of small non-coding RNAs that regulate many cellular processes, including pancreatic differentiation. Some miRNAs are known to be preferentially expressed in islets. Of note, miR-375 and miR-7 are two of the most abundant pancreatic miRNAs, and they are necessary for proper pancreatic islet development. Here we provide new insight into specific miRNAs involved in pancreatic differentiation. We found that miR-7 is differentially expressed during the differentiation of human embryonic stem cells (hESCs) into a beta cell-like phenotype and that its modulation plays an important role in generating mature pancreatic beta cells. This strategy may be exploited to optimize the potential for in vitro differentiation of hESCs into insulin-producing beta-like cells for use in preclinical studies and future clinical applications as well as the prospective uses of miRNAs to improve this process. American Society of Gene & Cell Therapy 2018-06-15 /pmc/articles/PMC6070677/ /pubmed/30195784 http://dx.doi.org/10.1016/j.omtn.2018.06.002 Text en © 2018 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article López-Beas, Javier Capilla-González, Vivian Aguilera, Yolanda Mellado, Nuria Lachaud, Christian C. Martín, Franz Smani, Tarik Soria, Bernat Hmadcha, Abdelkrim miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation |
title | miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation |
title_full | miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation |
title_fullStr | miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation |
title_full_unstemmed | miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation |
title_short | miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation |
title_sort | mir-7 modulates hesc differentiation into insulin-producing beta-like cells and contributes to cell maturation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070677/ https://www.ncbi.nlm.nih.gov/pubmed/30195784 http://dx.doi.org/10.1016/j.omtn.2018.06.002 |
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