Cargando…

Molecular Cloning and Effects of Tm14-3-3ζ-Silencing on Larval Survivability Against E. coli and C. albicans in Tenebrio molitor

The 14-3-3 family of proteins performs key regulatory functions in phosphorylation-dependent signaling pathways including cell survival and proliferation, apoptosis, regulation of chromatin structure and autophagy. In this study, the zeta isoform of 14-3-3 proteins (designated as Tm14-3-3ζ) was iden...

Descripción completa

Detalles Bibliográficos
Autores principales: Seong, Jeong Hwan, Jo, Yong Hun, Seo, Gi Won, Park, Soyi, Park, Ki Beom, Cho, Jun Ho, Ko, Hye Jin, Kim, Chang Eun, Patnaik, Bharat Bhusan, Jun, Sung Ah, Choi, Yong Seok, Kim, Young Wook, Bang, In Seok, Lee, Yong Seok, Han, Yeon Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070784/
https://www.ncbi.nlm.nih.gov/pubmed/29966317
http://dx.doi.org/10.3390/genes9070330
_version_ 1783343733135114240
author Seong, Jeong Hwan
Jo, Yong Hun
Seo, Gi Won
Park, Soyi
Park, Ki Beom
Cho, Jun Ho
Ko, Hye Jin
Kim, Chang Eun
Patnaik, Bharat Bhusan
Jun, Sung Ah
Choi, Yong Seok
Kim, Young Wook
Bang, In Seok
Lee, Yong Seok
Han, Yeon Soo
author_facet Seong, Jeong Hwan
Jo, Yong Hun
Seo, Gi Won
Park, Soyi
Park, Ki Beom
Cho, Jun Ho
Ko, Hye Jin
Kim, Chang Eun
Patnaik, Bharat Bhusan
Jun, Sung Ah
Choi, Yong Seok
Kim, Young Wook
Bang, In Seok
Lee, Yong Seok
Han, Yeon Soo
author_sort Seong, Jeong Hwan
collection PubMed
description The 14-3-3 family of proteins performs key regulatory functions in phosphorylation-dependent signaling pathways including cell survival and proliferation, apoptosis, regulation of chromatin structure and autophagy. In this study, the zeta isoform of 14-3-3 proteins (designated as Tm14-3-3ζ) was identified from the expressed sequence tags (ESTs) and RNA sequencing (RNA-Seq) database of the coleopteran pest, Tenebrio molitor. Tm14-3-3ζ messenger RNA (mRNA) is expressed at higher levels in the immune organs of the larval and adult stages of the insect and exhibit almost five-fold induction within 3 h post-infection of the larvae with Escherichia coli and Candida albicans. To investigate the biological function of Tm14-3-3ζ, a peptide-based Tm14-3-3ζ polyclonal antibody was generated in rabbit and the specificity was confirmed using Western blot analysis. Immunostaining and confocal microscopic analyses indicate that Tm14-3-3ζ is mainly expressed in the membranes of midgut epithelial cells, the nuclei of fat body and the cytosol of hemocytes. Gene silencing of Tm14-3-3ζ increases mortality of the larvae at 7 days post-infection with E. coli and C. albicans. Our findings demonstrate that 14-3-3ζ in T. molitor is essential in the host defense mechanisms against bacteria and fungi.
format Online
Article
Text
id pubmed-6070784
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-60707842018-08-09 Molecular Cloning and Effects of Tm14-3-3ζ-Silencing on Larval Survivability Against E. coli and C. albicans in Tenebrio molitor Seong, Jeong Hwan Jo, Yong Hun Seo, Gi Won Park, Soyi Park, Ki Beom Cho, Jun Ho Ko, Hye Jin Kim, Chang Eun Patnaik, Bharat Bhusan Jun, Sung Ah Choi, Yong Seok Kim, Young Wook Bang, In Seok Lee, Yong Seok Han, Yeon Soo Genes (Basel) Article The 14-3-3 family of proteins performs key regulatory functions in phosphorylation-dependent signaling pathways including cell survival and proliferation, apoptosis, regulation of chromatin structure and autophagy. In this study, the zeta isoform of 14-3-3 proteins (designated as Tm14-3-3ζ) was identified from the expressed sequence tags (ESTs) and RNA sequencing (RNA-Seq) database of the coleopteran pest, Tenebrio molitor. Tm14-3-3ζ messenger RNA (mRNA) is expressed at higher levels in the immune organs of the larval and adult stages of the insect and exhibit almost five-fold induction within 3 h post-infection of the larvae with Escherichia coli and Candida albicans. To investigate the biological function of Tm14-3-3ζ, a peptide-based Tm14-3-3ζ polyclonal antibody was generated in rabbit and the specificity was confirmed using Western blot analysis. Immunostaining and confocal microscopic analyses indicate that Tm14-3-3ζ is mainly expressed in the membranes of midgut epithelial cells, the nuclei of fat body and the cytosol of hemocytes. Gene silencing of Tm14-3-3ζ increases mortality of the larvae at 7 days post-infection with E. coli and C. albicans. Our findings demonstrate that 14-3-3ζ in T. molitor is essential in the host defense mechanisms against bacteria and fungi. MDPI 2018-06-29 /pmc/articles/PMC6070784/ /pubmed/29966317 http://dx.doi.org/10.3390/genes9070330 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Seong, Jeong Hwan
Jo, Yong Hun
Seo, Gi Won
Park, Soyi
Park, Ki Beom
Cho, Jun Ho
Ko, Hye Jin
Kim, Chang Eun
Patnaik, Bharat Bhusan
Jun, Sung Ah
Choi, Yong Seok
Kim, Young Wook
Bang, In Seok
Lee, Yong Seok
Han, Yeon Soo
Molecular Cloning and Effects of Tm14-3-3ζ-Silencing on Larval Survivability Against E. coli and C. albicans in Tenebrio molitor
title Molecular Cloning and Effects of Tm14-3-3ζ-Silencing on Larval Survivability Against E. coli and C. albicans in Tenebrio molitor
title_full Molecular Cloning and Effects of Tm14-3-3ζ-Silencing on Larval Survivability Against E. coli and C. albicans in Tenebrio molitor
title_fullStr Molecular Cloning and Effects of Tm14-3-3ζ-Silencing on Larval Survivability Against E. coli and C. albicans in Tenebrio molitor
title_full_unstemmed Molecular Cloning and Effects of Tm14-3-3ζ-Silencing on Larval Survivability Against E. coli and C. albicans in Tenebrio molitor
title_short Molecular Cloning and Effects of Tm14-3-3ζ-Silencing on Larval Survivability Against E. coli and C. albicans in Tenebrio molitor
title_sort molecular cloning and effects of tm14-3-3ζ-silencing on larval survivability against e. coli and c. albicans in tenebrio molitor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070784/
https://www.ncbi.nlm.nih.gov/pubmed/29966317
http://dx.doi.org/10.3390/genes9070330
work_keys_str_mv AT seongjeonghwan molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT joyonghun molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT seogiwon molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT parksoyi molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT parkkibeom molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT chojunho molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT kohyejin molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT kimchangeun molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT patnaikbharatbhusan molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT junsungah molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT choiyongseok molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT kimyoungwook molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT banginseok molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT leeyongseok molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor
AT hanyeonsoo molecularcloningandeffectsoftm1433zsilencingonlarvalsurvivabilityagainstecoliandcalbicansintenebriomolitor