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Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents

By coating surfaces with nano-crystalline diamond (NCD) particles, hydrophilicity can be altered via sidechain modifications without affecting surface texture. The present study aimed to assess the impact of NCD hydrophilicity on machined and rough SLA titanium discs on soft tissue integration, usin...

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Autores principales: Stigler, Robert Gerhard, Becker, Kathrin, Bruschi, Michela, Steinmüller-Nethl, Doris, Gassner, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070785/
https://www.ncbi.nlm.nih.gov/pubmed/30011802
http://dx.doi.org/10.3390/nano8070524
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author Stigler, Robert Gerhard
Becker, Kathrin
Bruschi, Michela
Steinmüller-Nethl, Doris
Gassner, Robert
author_facet Stigler, Robert Gerhard
Becker, Kathrin
Bruschi, Michela
Steinmüller-Nethl, Doris
Gassner, Robert
author_sort Stigler, Robert Gerhard
collection PubMed
description By coating surfaces with nano-crystalline diamond (NCD) particles, hydrophilicity can be altered via sidechain modifications without affecting surface texture. The present study aimed to assess the impact of NCD hydrophilicity on machined and rough SLA titanium discs on soft tissue integration, using a rodent model simulating submerged healing. Four different titanium discs (machined titanium = M Titanium, NCD-coated hydrophilic machined titanium = M-O-NCD, sand blasted acid etched (SLA Titanium) titanium, and hydrophilic NCD-coated SLA titanium = SLA O-NCD) were inserted in subdermal pockets of 12 Wistar rats. After one and four weeks of healing, the animals were sacrificed. Biopsies were embedded in methyl methacrylate (MMA), and processed for histology. The number of cells located within a region of interest (ROI) of 10 µm around the discs were counted and compared statistically. Signs of inflammation were evaluated descriptively employing immunohistochemistry. At one week, M-O-NCD coated titanium discs showed significantly higher amounts of cells compared to M Titanium, SLA Titanium, and SLA-O-NCD (p < 0.001). At four weeks, significant higher cell counts were noted at SLA-O-NCD surfaces (p < 0.01). Immunohistochemistry revealed decreased inflammatory responses at hydrophilic surfaces. Within the limits of an animal study, M-O-NCD surfaces seem to stimulate cell proliferation in the initial healing phase, whereas SLA-O-NCD surfaces appeared advantageous afterwards.
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spelling pubmed-60707852018-08-09 Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents Stigler, Robert Gerhard Becker, Kathrin Bruschi, Michela Steinmüller-Nethl, Doris Gassner, Robert Nanomaterials (Basel) Article By coating surfaces with nano-crystalline diamond (NCD) particles, hydrophilicity can be altered via sidechain modifications without affecting surface texture. The present study aimed to assess the impact of NCD hydrophilicity on machined and rough SLA titanium discs on soft tissue integration, using a rodent model simulating submerged healing. Four different titanium discs (machined titanium = M Titanium, NCD-coated hydrophilic machined titanium = M-O-NCD, sand blasted acid etched (SLA Titanium) titanium, and hydrophilic NCD-coated SLA titanium = SLA O-NCD) were inserted in subdermal pockets of 12 Wistar rats. After one and four weeks of healing, the animals were sacrificed. Biopsies were embedded in methyl methacrylate (MMA), and processed for histology. The number of cells located within a region of interest (ROI) of 10 µm around the discs were counted and compared statistically. Signs of inflammation were evaluated descriptively employing immunohistochemistry. At one week, M-O-NCD coated titanium discs showed significantly higher amounts of cells compared to M Titanium, SLA Titanium, and SLA-O-NCD (p < 0.001). At four weeks, significant higher cell counts were noted at SLA-O-NCD surfaces (p < 0.01). Immunohistochemistry revealed decreased inflammatory responses at hydrophilic surfaces. Within the limits of an animal study, M-O-NCD surfaces seem to stimulate cell proliferation in the initial healing phase, whereas SLA-O-NCD surfaces appeared advantageous afterwards. MDPI 2018-07-13 /pmc/articles/PMC6070785/ /pubmed/30011802 http://dx.doi.org/10.3390/nano8070524 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stigler, Robert Gerhard
Becker, Kathrin
Bruschi, Michela
Steinmüller-Nethl, Doris
Gassner, Robert
Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents
title Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents
title_full Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents
title_fullStr Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents
title_full_unstemmed Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents
title_short Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents
title_sort impact of nano-crystalline diamond enhanced hydrophilicity on cell proliferation on machined and sla titanium surfaces: an in-vivo study in rodents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070785/
https://www.ncbi.nlm.nih.gov/pubmed/30011802
http://dx.doi.org/10.3390/nano8070524
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