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NF-kappaB Regulates Redox Status in Breast Cancer Subtypes

Oxidative stress (OS) is an indispensable condition to ensure genomic instability in cancer cells. In breast cancer (BC), redox alterations have been widely characterized, but since this process results from a chain of inflammatory events, the causal molecular triggers remain to be identified. In th...

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Detalles Bibliográficos
Autores principales: Pires, Bruno R. B., Binato, Renata, Ferreira, Gerson M., Cecchini, Rubens, Panis, Carolina, Abdelhay, Eliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070792/
https://www.ncbi.nlm.nih.gov/pubmed/29949949
http://dx.doi.org/10.3390/genes9070320
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author Pires, Bruno R. B.
Binato, Renata
Ferreira, Gerson M.
Cecchini, Rubens
Panis, Carolina
Abdelhay, Eliana
author_facet Pires, Bruno R. B.
Binato, Renata
Ferreira, Gerson M.
Cecchini, Rubens
Panis, Carolina
Abdelhay, Eliana
author_sort Pires, Bruno R. B.
collection PubMed
description Oxidative stress (OS) is an indispensable condition to ensure genomic instability in cancer cells. In breast cancer (BC), redox alterations have been widely characterized, but since this process results from a chain of inflammatory events, the causal molecular triggers remain to be identified. In this context, we used a microarray approach to investigate the role of the main pro-oxidant transcription factor, nuclear factor-kappa B (NF-κB), in gene profiles of BC subtypes. Our results showed that NF-κB knockdown in distinct BC subtypes led to differential expression of relevant factors involved in glutathione metabolism, prostaglandins, cytochrome P450 and cyclooxygenase, suggesting a relationship between the redox balance and NF-κB in such cells. In addition, we performed biochemical analyses to validate the microarray dataset focusing on OS and correlated these parameters with normal expression or NF-κB inhibition. Our data showed a distinct oxidative status pattern for each of the three studied BC subtype models, consistent with the intrinsic characteristics of each BC subtype. Thus, our findings suggest that NF-κB may represent an additional mechanism related to OS maintenance in BC, operating in various forms to mediate other important predominant signaling components of each BC subtype.
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spelling pubmed-60707922018-08-09 NF-kappaB Regulates Redox Status in Breast Cancer Subtypes Pires, Bruno R. B. Binato, Renata Ferreira, Gerson M. Cecchini, Rubens Panis, Carolina Abdelhay, Eliana Genes (Basel) Article Oxidative stress (OS) is an indispensable condition to ensure genomic instability in cancer cells. In breast cancer (BC), redox alterations have been widely characterized, but since this process results from a chain of inflammatory events, the causal molecular triggers remain to be identified. In this context, we used a microarray approach to investigate the role of the main pro-oxidant transcription factor, nuclear factor-kappa B (NF-κB), in gene profiles of BC subtypes. Our results showed that NF-κB knockdown in distinct BC subtypes led to differential expression of relevant factors involved in glutathione metabolism, prostaglandins, cytochrome P450 and cyclooxygenase, suggesting a relationship between the redox balance and NF-κB in such cells. In addition, we performed biochemical analyses to validate the microarray dataset focusing on OS and correlated these parameters with normal expression or NF-κB inhibition. Our data showed a distinct oxidative status pattern for each of the three studied BC subtype models, consistent with the intrinsic characteristics of each BC subtype. Thus, our findings suggest that NF-κB may represent an additional mechanism related to OS maintenance in BC, operating in various forms to mediate other important predominant signaling components of each BC subtype. MDPI 2018-06-26 /pmc/articles/PMC6070792/ /pubmed/29949949 http://dx.doi.org/10.3390/genes9070320 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pires, Bruno R. B.
Binato, Renata
Ferreira, Gerson M.
Cecchini, Rubens
Panis, Carolina
Abdelhay, Eliana
NF-kappaB Regulates Redox Status in Breast Cancer Subtypes
title NF-kappaB Regulates Redox Status in Breast Cancer Subtypes
title_full NF-kappaB Regulates Redox Status in Breast Cancer Subtypes
title_fullStr NF-kappaB Regulates Redox Status in Breast Cancer Subtypes
title_full_unstemmed NF-kappaB Regulates Redox Status in Breast Cancer Subtypes
title_short NF-kappaB Regulates Redox Status in Breast Cancer Subtypes
title_sort nf-kappab regulates redox status in breast cancer subtypes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070792/
https://www.ncbi.nlm.nih.gov/pubmed/29949949
http://dx.doi.org/10.3390/genes9070320
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