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Unilateral Botulinum Neurotoxin-A Injection into the Striatum of C57BL/6 Mice Leads to a Different Motor Behavior Compared with Rats

Different morphological changes in the caudate-putamen (CPu) of naïve rats and mice were observed after intrastriatal botulinum neurotoxin-A (BoNT-A) injection. For this purpose we here studied various motor behaviors in mice (n = 46) longitudinally up to 9 months after intrastriatal BoNT-A administ...

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Autores principales: Antipova, Veronica, Wree, Andreas, Holzmann, Carsten, Mann, Teresa, Palomero-Gallagher, Nicola, Zilles, Karl, Schmitt, Oliver, Hawlitschka, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070800/
https://www.ncbi.nlm.nih.gov/pubmed/30018211
http://dx.doi.org/10.3390/toxins10070295
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author Antipova, Veronica
Wree, Andreas
Holzmann, Carsten
Mann, Teresa
Palomero-Gallagher, Nicola
Zilles, Karl
Schmitt, Oliver
Hawlitschka, Alexander
author_facet Antipova, Veronica
Wree, Andreas
Holzmann, Carsten
Mann, Teresa
Palomero-Gallagher, Nicola
Zilles, Karl
Schmitt, Oliver
Hawlitschka, Alexander
author_sort Antipova, Veronica
collection PubMed
description Different morphological changes in the caudate-putamen (CPu) of naïve rats and mice were observed after intrastriatal botulinum neurotoxin-A (BoNT-A) injection. For this purpose we here studied various motor behaviors in mice (n = 46) longitudinally up to 9 months after intrastriatal BoNT-A administration as previously reported for rats, and compared both outcomes. Apomorphine- and amphetamine-induced rotational behavior, spontaneous motor behavior, as well as lateralized neglect were studied in mice after the injection of single doses of BoNT-A into the right CPu, comparing them with sham-injected animals. Unilateral intrastriatal injection of BoNT-A in mice induced significantly increased contralateral apomorphine-induced rotations for 1 to 3 months, as well as significantly increased contralateral amphetamine-induced rotations 1 to 9 months after injection. In rats (n = 28), unilateral BoNT-A injection also induced significantly increased contralateral apomorphine-induced rotations 3 months after injection, but did not provoke amphetamine-induced rotations at all. Lateralized sensorimotor integration, forelimb preference, and forelimb stepping were significantly impaired on the left side. The differences in motor behaviors between rats and mice may be caused by different BoNT-A effects on cholinergic and catecholaminergic fibers in rat and mouse striata, interspecies differences in striatal receptor densities, and different connectomes of the basal ganglia.
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spelling pubmed-60708002018-08-09 Unilateral Botulinum Neurotoxin-A Injection into the Striatum of C57BL/6 Mice Leads to a Different Motor Behavior Compared with Rats Antipova, Veronica Wree, Andreas Holzmann, Carsten Mann, Teresa Palomero-Gallagher, Nicola Zilles, Karl Schmitt, Oliver Hawlitschka, Alexander Toxins (Basel) Article Different morphological changes in the caudate-putamen (CPu) of naïve rats and mice were observed after intrastriatal botulinum neurotoxin-A (BoNT-A) injection. For this purpose we here studied various motor behaviors in mice (n = 46) longitudinally up to 9 months after intrastriatal BoNT-A administration as previously reported for rats, and compared both outcomes. Apomorphine- and amphetamine-induced rotational behavior, spontaneous motor behavior, as well as lateralized neglect were studied in mice after the injection of single doses of BoNT-A into the right CPu, comparing them with sham-injected animals. Unilateral intrastriatal injection of BoNT-A in mice induced significantly increased contralateral apomorphine-induced rotations for 1 to 3 months, as well as significantly increased contralateral amphetamine-induced rotations 1 to 9 months after injection. In rats (n = 28), unilateral BoNT-A injection also induced significantly increased contralateral apomorphine-induced rotations 3 months after injection, but did not provoke amphetamine-induced rotations at all. Lateralized sensorimotor integration, forelimb preference, and forelimb stepping were significantly impaired on the left side. The differences in motor behaviors between rats and mice may be caused by different BoNT-A effects on cholinergic and catecholaminergic fibers in rat and mouse striata, interspecies differences in striatal receptor densities, and different connectomes of the basal ganglia. MDPI 2018-07-17 /pmc/articles/PMC6070800/ /pubmed/30018211 http://dx.doi.org/10.3390/toxins10070295 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Antipova, Veronica
Wree, Andreas
Holzmann, Carsten
Mann, Teresa
Palomero-Gallagher, Nicola
Zilles, Karl
Schmitt, Oliver
Hawlitschka, Alexander
Unilateral Botulinum Neurotoxin-A Injection into the Striatum of C57BL/6 Mice Leads to a Different Motor Behavior Compared with Rats
title Unilateral Botulinum Neurotoxin-A Injection into the Striatum of C57BL/6 Mice Leads to a Different Motor Behavior Compared with Rats
title_full Unilateral Botulinum Neurotoxin-A Injection into the Striatum of C57BL/6 Mice Leads to a Different Motor Behavior Compared with Rats
title_fullStr Unilateral Botulinum Neurotoxin-A Injection into the Striatum of C57BL/6 Mice Leads to a Different Motor Behavior Compared with Rats
title_full_unstemmed Unilateral Botulinum Neurotoxin-A Injection into the Striatum of C57BL/6 Mice Leads to a Different Motor Behavior Compared with Rats
title_short Unilateral Botulinum Neurotoxin-A Injection into the Striatum of C57BL/6 Mice Leads to a Different Motor Behavior Compared with Rats
title_sort unilateral botulinum neurotoxin-a injection into the striatum of c57bl/6 mice leads to a different motor behavior compared with rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070800/
https://www.ncbi.nlm.nih.gov/pubmed/30018211
http://dx.doi.org/10.3390/toxins10070295
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