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Differential Gene Expression Profile Induced by Valproic Acid (VPA) in Pediatric Epileptic Patients
Epilepsy is a neuronal disease that affects up to 70 million people worldwide. The development of effective therapies to combat childhood epilepsy requires early biomarkers. Here, we performed a whole-genome microarray analysis in blood cells to identify genes differentially expressed between epilep...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070821/ https://www.ncbi.nlm.nih.gov/pubmed/29958461 http://dx.doi.org/10.3390/genes9070328 |
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author | Floriano-Sánchez, Esaú Brindis, Fernando Ortega-Cuellar, Daniel Ignacio-Mejía, Ivan Moreno-Arriola, Elizabeth Romero-Morelos, Pablo Ceballos-Vasquez, Edgar Córdova-Espinoza, María Guadalupe Arregoitia-Sarabia, Cindy Karel Sandoval-Pacheco, Roberto Carmona-Aparicio, Liliana Cárdenas-Rodríguez, Noemí |
author_facet | Floriano-Sánchez, Esaú Brindis, Fernando Ortega-Cuellar, Daniel Ignacio-Mejía, Ivan Moreno-Arriola, Elizabeth Romero-Morelos, Pablo Ceballos-Vasquez, Edgar Córdova-Espinoza, María Guadalupe Arregoitia-Sarabia, Cindy Karel Sandoval-Pacheco, Roberto Carmona-Aparicio, Liliana Cárdenas-Rodríguez, Noemí |
author_sort | Floriano-Sánchez, Esaú |
collection | PubMed |
description | Epilepsy is a neuronal disease that affects up to 70 million people worldwide. The development of effective therapies to combat childhood epilepsy requires early biomarkers. Here, we performed a whole-genome microarray analysis in blood cells to identify genes differentially expressed between epileptic and epileptic valproic acid (VPA)-treated children versus normal children to obtain information about the gene expression to help us to understand genetic aspects of this disease. We found that the most significant differentially expressed genes were related to the transcriptional factor cAMP-response element binding protein (CREB) that is overexpressed in children with epilepsy compared with normal children, and 6 and 12 months of VPA treatment reversed several of these changes. Interestingly, leukocyte-associated immunoglobulin-like receptor 1 (LAIR1), a type I transmembrane glycoprotein that binds collagen proteins and contains CREB binding sites, was one of the more up-regulated genes in epileptic patients, and treatment with VPA strongly reversed its up-regulation. CREB up-regulates genes related to epilepsy; here, we suggest that LAIR1 could activate CREB, and together, they trigger epilepsy. After VPA treatment, LAIR1 repressed genes by disrupting the functional LAIR1–CREB complex, resulting in successful treatment. A functional microarray analysis offers new information that could open novel avenues of research in biomarker discovery, which may be useful for the early identification of children with a predisposition to epilepsy. |
format | Online Article Text |
id | pubmed-6070821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60708212018-08-09 Differential Gene Expression Profile Induced by Valproic Acid (VPA) in Pediatric Epileptic Patients Floriano-Sánchez, Esaú Brindis, Fernando Ortega-Cuellar, Daniel Ignacio-Mejía, Ivan Moreno-Arriola, Elizabeth Romero-Morelos, Pablo Ceballos-Vasquez, Edgar Córdova-Espinoza, María Guadalupe Arregoitia-Sarabia, Cindy Karel Sandoval-Pacheco, Roberto Carmona-Aparicio, Liliana Cárdenas-Rodríguez, Noemí Genes (Basel) Article Epilepsy is a neuronal disease that affects up to 70 million people worldwide. The development of effective therapies to combat childhood epilepsy requires early biomarkers. Here, we performed a whole-genome microarray analysis in blood cells to identify genes differentially expressed between epileptic and epileptic valproic acid (VPA)-treated children versus normal children to obtain information about the gene expression to help us to understand genetic aspects of this disease. We found that the most significant differentially expressed genes were related to the transcriptional factor cAMP-response element binding protein (CREB) that is overexpressed in children with epilepsy compared with normal children, and 6 and 12 months of VPA treatment reversed several of these changes. Interestingly, leukocyte-associated immunoglobulin-like receptor 1 (LAIR1), a type I transmembrane glycoprotein that binds collagen proteins and contains CREB binding sites, was one of the more up-regulated genes in epileptic patients, and treatment with VPA strongly reversed its up-regulation. CREB up-regulates genes related to epilepsy; here, we suggest that LAIR1 could activate CREB, and together, they trigger epilepsy. After VPA treatment, LAIR1 repressed genes by disrupting the functional LAIR1–CREB complex, resulting in successful treatment. A functional microarray analysis offers new information that could open novel avenues of research in biomarker discovery, which may be useful for the early identification of children with a predisposition to epilepsy. MDPI 2018-06-28 /pmc/articles/PMC6070821/ /pubmed/29958461 http://dx.doi.org/10.3390/genes9070328 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Floriano-Sánchez, Esaú Brindis, Fernando Ortega-Cuellar, Daniel Ignacio-Mejía, Ivan Moreno-Arriola, Elizabeth Romero-Morelos, Pablo Ceballos-Vasquez, Edgar Córdova-Espinoza, María Guadalupe Arregoitia-Sarabia, Cindy Karel Sandoval-Pacheco, Roberto Carmona-Aparicio, Liliana Cárdenas-Rodríguez, Noemí Differential Gene Expression Profile Induced by Valproic Acid (VPA) in Pediatric Epileptic Patients |
title | Differential Gene Expression Profile Induced by Valproic Acid (VPA) in Pediatric Epileptic Patients |
title_full | Differential Gene Expression Profile Induced by Valproic Acid (VPA) in Pediatric Epileptic Patients |
title_fullStr | Differential Gene Expression Profile Induced by Valproic Acid (VPA) in Pediatric Epileptic Patients |
title_full_unstemmed | Differential Gene Expression Profile Induced by Valproic Acid (VPA) in Pediatric Epileptic Patients |
title_short | Differential Gene Expression Profile Induced by Valproic Acid (VPA) in Pediatric Epileptic Patients |
title_sort | differential gene expression profile induced by valproic acid (vpa) in pediatric epileptic patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070821/ https://www.ncbi.nlm.nih.gov/pubmed/29958461 http://dx.doi.org/10.3390/genes9070328 |
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