Cargando…
Characterisation of tumour microvessel density during progression of high-grade serous ovarian cancer: clinico-pathological impact (an OCTIPS Consortium study).
BACKGROUND: High-grade serous ovarian cancer (HGSOC) intratumoural vasculature evolution remains unknown. The study investigated changes in tumour microvessel density (MVD) in a large cohort of paired primary and recurrent HGSOC tissue samples and its impact on patients’ clinico-pathological outcome...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070919/ https://www.ncbi.nlm.nih.gov/pubmed/29955134 http://dx.doi.org/10.1038/s41416-018-0157-z |
_version_ | 1783343764813643776 |
---|---|
author | Ruscito, Ilary Cacsire Castillo-Tong, Dan Vergote, Ignace Ignat, Iulia Stanske, Mandy Vanderstichele, Adriaan Glajzer, Jacek Kulbe, Hagen Trillsch, Fabian Mustea, Alexander Kreuzinger, Caroline Benedetti Panici, Pierluigi Gourley, Charlie Gabra, Hani Nuti, Marianna Taube, Eliane T. Kessler, Mirjana Sehouli, Jalid Darb-Esfahani, Silvia Braicu, Elena Ioana |
author_facet | Ruscito, Ilary Cacsire Castillo-Tong, Dan Vergote, Ignace Ignat, Iulia Stanske, Mandy Vanderstichele, Adriaan Glajzer, Jacek Kulbe, Hagen Trillsch, Fabian Mustea, Alexander Kreuzinger, Caroline Benedetti Panici, Pierluigi Gourley, Charlie Gabra, Hani Nuti, Marianna Taube, Eliane T. Kessler, Mirjana Sehouli, Jalid Darb-Esfahani, Silvia Braicu, Elena Ioana |
author_sort | Ruscito, Ilary |
collection | PubMed |
description | BACKGROUND: High-grade serous ovarian cancer (HGSOC) intratumoural vasculature evolution remains unknown. The study investigated changes in tumour microvessel density (MVD) in a large cohort of paired primary and recurrent HGSOC tissue samples and its impact on patients’ clinico-pathological outcome. METHODS: A total of 222 primary (pOC) and recurrent (rOC) intra-patient paired HGSOC were assessed for immunohistochemical expression of angiogenesis-associated biomarkers (CD31, to evaluate MVD, and VEGF-A). Expression profiles were compared between pOCs and rOCs and correlated with patients' data. RESULTS: High intratumoural MVD and VEGF-A expression were observed in 75.7% (84/111) and 20.7% (23/111) pOCs, respectively. MVD(high) and VEGF((+)) samples were detected in 51.4% (57/111) and 20.7% (23/111) rOCs, respectively. MVD(high)/VEGF((+)) co-expression was found in 19.8% (22/111) and 8.1% (9/111) of pOCs and rOCs, respectively (p = 0.02). Pairwise analysis showed no significant change in MVD (p = 0.935) and VEGF-A (p = 0.121) levels from pOCs to rOCs. MVD(high) pOCs were associated with higher CD3((+)) (p = 0.029) and CD8((+)) (p = 0.013) intratumoural effector TILs, while VEGF((+)) samples were most frequently encountered among BRCA-mutated tumours (p = 0.019). Multivariate analysis showed VEGF and MVD were not independent prognostic factors for OS. CONCLUSIONS: HGSOC intratumoural vasculature did not undergo significant changes during disease progression. High concentration of CD31((+)) vessels seems to promote recruitment of effector TILs. The study also provides preliminary evidence of the correlation between VEGF-positivity and BRCA status. |
format | Online Article Text |
id | pubmed-6070919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60709192019-08-01 Characterisation of tumour microvessel density during progression of high-grade serous ovarian cancer: clinico-pathological impact (an OCTIPS Consortium study). Ruscito, Ilary Cacsire Castillo-Tong, Dan Vergote, Ignace Ignat, Iulia Stanske, Mandy Vanderstichele, Adriaan Glajzer, Jacek Kulbe, Hagen Trillsch, Fabian Mustea, Alexander Kreuzinger, Caroline Benedetti Panici, Pierluigi Gourley, Charlie Gabra, Hani Nuti, Marianna Taube, Eliane T. Kessler, Mirjana Sehouli, Jalid Darb-Esfahani, Silvia Braicu, Elena Ioana Br J Cancer Article BACKGROUND: High-grade serous ovarian cancer (HGSOC) intratumoural vasculature evolution remains unknown. The study investigated changes in tumour microvessel density (MVD) in a large cohort of paired primary and recurrent HGSOC tissue samples and its impact on patients’ clinico-pathological outcome. METHODS: A total of 222 primary (pOC) and recurrent (rOC) intra-patient paired HGSOC were assessed for immunohistochemical expression of angiogenesis-associated biomarkers (CD31, to evaluate MVD, and VEGF-A). Expression profiles were compared between pOCs and rOCs and correlated with patients' data. RESULTS: High intratumoural MVD and VEGF-A expression were observed in 75.7% (84/111) and 20.7% (23/111) pOCs, respectively. MVD(high) and VEGF((+)) samples were detected in 51.4% (57/111) and 20.7% (23/111) rOCs, respectively. MVD(high)/VEGF((+)) co-expression was found in 19.8% (22/111) and 8.1% (9/111) of pOCs and rOCs, respectively (p = 0.02). Pairwise analysis showed no significant change in MVD (p = 0.935) and VEGF-A (p = 0.121) levels from pOCs to rOCs. MVD(high) pOCs were associated with higher CD3((+)) (p = 0.029) and CD8((+)) (p = 0.013) intratumoural effector TILs, while VEGF((+)) samples were most frequently encountered among BRCA-mutated tumours (p = 0.019). Multivariate analysis showed VEGF and MVD were not independent prognostic factors for OS. CONCLUSIONS: HGSOC intratumoural vasculature did not undergo significant changes during disease progression. High concentration of CD31((+)) vessels seems to promote recruitment of effector TILs. The study also provides preliminary evidence of the correlation between VEGF-positivity and BRCA status. Nature Publishing Group UK 2018-06-29 2018-08-01 /pmc/articles/PMC6070919/ /pubmed/29955134 http://dx.doi.org/10.1038/s41416-018-0157-z Text en © Cancer Research UK 2018 https://creativecommons.org/licenses/by/4.0/This work is published under the standard license to publish agreement. After 12 months the work will become freely available and the license terms will switch to a Creative Commons Attribution 4.0 International (CC BY 4.0). |
spellingShingle | Article Ruscito, Ilary Cacsire Castillo-Tong, Dan Vergote, Ignace Ignat, Iulia Stanske, Mandy Vanderstichele, Adriaan Glajzer, Jacek Kulbe, Hagen Trillsch, Fabian Mustea, Alexander Kreuzinger, Caroline Benedetti Panici, Pierluigi Gourley, Charlie Gabra, Hani Nuti, Marianna Taube, Eliane T. Kessler, Mirjana Sehouli, Jalid Darb-Esfahani, Silvia Braicu, Elena Ioana Characterisation of tumour microvessel density during progression of high-grade serous ovarian cancer: clinico-pathological impact (an OCTIPS Consortium study). |
title | Characterisation of tumour microvessel density during progression of high-grade serous ovarian cancer: clinico-pathological impact (an OCTIPS Consortium study). |
title_full | Characterisation of tumour microvessel density during progression of high-grade serous ovarian cancer: clinico-pathological impact (an OCTIPS Consortium study). |
title_fullStr | Characterisation of tumour microvessel density during progression of high-grade serous ovarian cancer: clinico-pathological impact (an OCTIPS Consortium study). |
title_full_unstemmed | Characterisation of tumour microvessel density during progression of high-grade serous ovarian cancer: clinico-pathological impact (an OCTIPS Consortium study). |
title_short | Characterisation of tumour microvessel density during progression of high-grade serous ovarian cancer: clinico-pathological impact (an OCTIPS Consortium study). |
title_sort | characterisation of tumour microvessel density during progression of high-grade serous ovarian cancer: clinico-pathological impact (an octips consortium study). |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070919/ https://www.ncbi.nlm.nih.gov/pubmed/29955134 http://dx.doi.org/10.1038/s41416-018-0157-z |
work_keys_str_mv | AT ruscitoilary characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT cacsirecastillotongdan characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT vergoteignace characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT ignatiulia characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT stanskemandy characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT vandersticheleadriaan characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT glajzerjacek characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT kulbehagen characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT trillschfabian characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT musteaalexander characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT kreuzingercaroline characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT benedettipanicipierluigi characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT gourleycharlie characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT gabrahani characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT nutimarianna characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT taubeelianet characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT kesslermirjana characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT sehoulijalid characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT darbesfahanisilvia characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy AT braicuelenaioana characterisationoftumourmicrovesseldensityduringprogressionofhighgradeserousovariancancerclinicopathologicalimpactanoctipsconsortiumstudy |