Cargando…
High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics
Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described,...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070981/ https://www.ncbi.nlm.nih.gov/pubmed/30037079 http://dx.doi.org/10.3390/v10070385 |
_version_ | 1783343779230515200 |
---|---|
author | Katsiani, Asimina Maliogka, Varvara I. Katis, Nikolaos Svanella-Dumas, Laurence Olmos, Antonio Ruiz-García, Ana B. Marais, Armelle Faure, Chantal Theil, Sébastien Lotos, Leonidas Candresse, Thierry |
author_facet | Katsiani, Asimina Maliogka, Varvara I. Katis, Nikolaos Svanella-Dumas, Laurence Olmos, Antonio Ruiz-García, Ana B. Marais, Armelle Faure, Chantal Theil, Sébastien Lotos, Leonidas Candresse, Thierry |
author_sort | Katsiani, Asimina |
collection | PubMed |
description | Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches. Our results reveal the presence of further genetic diversity within the LChV1 species. Interestingly, mixed infections of the same sweet cherry tree with different LChV1 variants were identified for the first time. Taken together, the high intra-host and intra-species diversities of LChV1 might affect its pathogenicity and have clear implications for its accurate diagnostics. |
format | Online Article Text |
id | pubmed-6070981 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60709812018-08-09 High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics Katsiani, Asimina Maliogka, Varvara I. Katis, Nikolaos Svanella-Dumas, Laurence Olmos, Antonio Ruiz-García, Ana B. Marais, Armelle Faure, Chantal Theil, Sébastien Lotos, Leonidas Candresse, Thierry Viruses Article Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches. Our results reveal the presence of further genetic diversity within the LChV1 species. Interestingly, mixed infections of the same sweet cherry tree with different LChV1 variants were identified for the first time. Taken together, the high intra-host and intra-species diversities of LChV1 might affect its pathogenicity and have clear implications for its accurate diagnostics. MDPI 2018-07-21 /pmc/articles/PMC6070981/ /pubmed/30037079 http://dx.doi.org/10.3390/v10070385 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Katsiani, Asimina Maliogka, Varvara I. Katis, Nikolaos Svanella-Dumas, Laurence Olmos, Antonio Ruiz-García, Ana B. Marais, Armelle Faure, Chantal Theil, Sébastien Lotos, Leonidas Candresse, Thierry High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
title | High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
title_full | High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
title_fullStr | High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
title_full_unstemmed | High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
title_short | High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
title_sort | high-throughput sequencing reveals further diversity of little cherry virus 1 with implications for diagnostics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070981/ https://www.ncbi.nlm.nih.gov/pubmed/30037079 http://dx.doi.org/10.3390/v10070385 |
work_keys_str_mv | AT katsianiasimina highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics AT maliogkavarvarai highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics AT katisnikolaos highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics AT svanelladumaslaurence highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics AT olmosantonio highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics AT ruizgarciaanab highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics AT maraisarmelle highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics AT faurechantal highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics AT theilsebastien highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics AT lotosleonidas highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics AT candressethierry highthroughputsequencingrevealsfurtherdiversityoflittlecherryvirus1withimplicationsfordiagnostics |