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Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles
Microcystis aeruginosa is the most common species responsible for toxic cyanobacterial blooms and is considered a significant contributor to the production of cyanotoxins, particularly the potent liver toxins called microcystins. Numerous studies investigating Microcystis spp. blooms have revealed t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070999/ https://www.ncbi.nlm.nih.gov/pubmed/30041444 http://dx.doi.org/10.3390/toxins10070304 |
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author | Qu, Jiangqi Shen, Liping Zhao, Meng Li, Wentong Jia, Chengxia Zhu, Hua Zhang, Qingjing |
author_facet | Qu, Jiangqi Shen, Liping Zhao, Meng Li, Wentong Jia, Chengxia Zhu, Hua Zhang, Qingjing |
author_sort | Qu, Jiangqi |
collection | PubMed |
description | Microcystis aeruginosa is the most common species responsible for toxic cyanobacterial blooms and is considered a significant contributor to the production of cyanotoxins, particularly the potent liver toxins called microcystins. Numerous studies investigating Microcystis spp. blooms have revealed their deleterious effects in freshwater environments. However, the available knowledge regarding the global phosphoproteomics of M. aeruginosa and their regulatory roles in toxin generation is limited. In this study, we conducted comparative phosphoproteomic profiling of non-toxic and toxin-producing strains of M. aeruginosa. We identified 59 phosphorylation sites in 37 proteins in a non-toxic strain and 26 phosphorylation sites in 18 proteins in a toxin-producing strain. The analysis of protein phosphorylation abundances and functions in redox homeostasis, energy metabolism, light absorption and photosynthesis showed marked differences between the non-toxic and toxin-producing strains of M. aeruginosa, indicating that these processes are strongly related to toxin generation. Moreover, the protein-protein interaction results indicated that BJ0JVG8 can directly interact with the PemK-like toxin protein B0JQN8. Thus, the phosphorylation of B0JQN8 appears to be associated with the regulatory roles of toxins in physiological activity. |
format | Online Article Text |
id | pubmed-6070999 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60709992018-08-09 Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles Qu, Jiangqi Shen, Liping Zhao, Meng Li, Wentong Jia, Chengxia Zhu, Hua Zhang, Qingjing Toxins (Basel) Article Microcystis aeruginosa is the most common species responsible for toxic cyanobacterial blooms and is considered a significant contributor to the production of cyanotoxins, particularly the potent liver toxins called microcystins. Numerous studies investigating Microcystis spp. blooms have revealed their deleterious effects in freshwater environments. However, the available knowledge regarding the global phosphoproteomics of M. aeruginosa and their regulatory roles in toxin generation is limited. In this study, we conducted comparative phosphoproteomic profiling of non-toxic and toxin-producing strains of M. aeruginosa. We identified 59 phosphorylation sites in 37 proteins in a non-toxic strain and 26 phosphorylation sites in 18 proteins in a toxin-producing strain. The analysis of protein phosphorylation abundances and functions in redox homeostasis, energy metabolism, light absorption and photosynthesis showed marked differences between the non-toxic and toxin-producing strains of M. aeruginosa, indicating that these processes are strongly related to toxin generation. Moreover, the protein-protein interaction results indicated that BJ0JVG8 can directly interact with the PemK-like toxin protein B0JQN8. Thus, the phosphorylation of B0JQN8 appears to be associated with the regulatory roles of toxins in physiological activity. MDPI 2018-07-23 /pmc/articles/PMC6070999/ /pubmed/30041444 http://dx.doi.org/10.3390/toxins10070304 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Qu, Jiangqi Shen, Liping Zhao, Meng Li, Wentong Jia, Chengxia Zhu, Hua Zhang, Qingjing Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles |
title | Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles |
title_full | Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles |
title_fullStr | Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles |
title_full_unstemmed | Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles |
title_short | Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles |
title_sort | determination of the role of microcystis aeruginosa in toxin generation based on phosphoproteomic profiles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070999/ https://www.ncbi.nlm.nih.gov/pubmed/30041444 http://dx.doi.org/10.3390/toxins10070304 |
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