Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species

Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including p...

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Detalles Bibliográficos
Autores principales: Koloniuk, Igor, Thekke-Veetil, Thanuja, Reynard, Jean-Sébastien, Mavrič Pleško, Irena, Přibylová, Jaroslava, Brodard, Justine, Kellenberger, Isabelle, Sarkisova, Tatiana, Špak, Josef, Lamovšek, Janja, Massart, Sebastien, Ho, Thien, Postman, Joseph D., Tzanetakis, Ioannis E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071065/
https://www.ncbi.nlm.nih.gov/pubmed/30002359
http://dx.doi.org/10.3390/v10070369
Descripción
Sumario:Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.

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