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Neurogenic Differentiation of Human Dental Pulp Stem Cells on Graphene-Polycaprolactone Hybrid Nanofibers

Stem cells derived from dental tissues—dental stem cells—are favored due to their easy acquisition. Among them, dental pulp stem cells (DPSCs) extracted from the dental pulp have many advantages, such as high proliferation and a highly purified population. Although their ability for neurogenic diffe...

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Autores principales: Seonwoo, Hoon, Jang, Kyung-Je, Lee, Dohyeon, Park, Sunho, Lee, Myungchul, Park, Sangbae, Lim, Ki-Taek, Kim, Jangho, Chung, Jong Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071115/
https://www.ncbi.nlm.nih.gov/pubmed/30037100
http://dx.doi.org/10.3390/nano8070554
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author Seonwoo, Hoon
Jang, Kyung-Je
Lee, Dohyeon
Park, Sunho
Lee, Myungchul
Park, Sangbae
Lim, Ki-Taek
Kim, Jangho
Chung, Jong Hoon
author_facet Seonwoo, Hoon
Jang, Kyung-Je
Lee, Dohyeon
Park, Sunho
Lee, Myungchul
Park, Sangbae
Lim, Ki-Taek
Kim, Jangho
Chung, Jong Hoon
author_sort Seonwoo, Hoon
collection PubMed
description Stem cells derived from dental tissues—dental stem cells—are favored due to their easy acquisition. Among them, dental pulp stem cells (DPSCs) extracted from the dental pulp have many advantages, such as high proliferation and a highly purified population. Although their ability for neurogenic differentiation has been highlighted and neurogenic differentiation using electrospun nanofibers (NFs) has been performed, graphene-incorporated NFs have never been applied for DPSC neurogenic differentiation. Here, reduced graphene oxide (RGO)-polycaprolactone (PCL) hybrid electrospun NFs were developed and applied for enhanced neurogenesis of DPSCs. First, RGO-PCL NFs were fabricated by electrospinning with incorporation of RGO and alignments, and their chemical and morphological characteristics were evaluated. Furthermore, in vitro NF properties, such as influence on the cellular alignments and cell viability of DPSCs, were also analyzed. The influences of NFs on DPSCs neurogenesis were also analyzed. The results confirmed that an appropriate concentration of RGO promoted better DPSC neurogenesis. Furthermore, the use of random NFs facilitated contiguous junctions of differentiated cells, whereas the use of aligned NFs facilitated an aligned junction of differentiated cells along the direction of NF alignments. Our findings showed that RGO-PCL NFs can be a useful tool for DPSC neurogenesis, which will help regeneration in neurodegenerative and neurodefective diseases.
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spelling pubmed-60711152018-08-09 Neurogenic Differentiation of Human Dental Pulp Stem Cells on Graphene-Polycaprolactone Hybrid Nanofibers Seonwoo, Hoon Jang, Kyung-Je Lee, Dohyeon Park, Sunho Lee, Myungchul Park, Sangbae Lim, Ki-Taek Kim, Jangho Chung, Jong Hoon Nanomaterials (Basel) Communication Stem cells derived from dental tissues—dental stem cells—are favored due to their easy acquisition. Among them, dental pulp stem cells (DPSCs) extracted from the dental pulp have many advantages, such as high proliferation and a highly purified population. Although their ability for neurogenic differentiation has been highlighted and neurogenic differentiation using electrospun nanofibers (NFs) has been performed, graphene-incorporated NFs have never been applied for DPSC neurogenic differentiation. Here, reduced graphene oxide (RGO)-polycaprolactone (PCL) hybrid electrospun NFs were developed and applied for enhanced neurogenesis of DPSCs. First, RGO-PCL NFs were fabricated by electrospinning with incorporation of RGO and alignments, and their chemical and morphological characteristics were evaluated. Furthermore, in vitro NF properties, such as influence on the cellular alignments and cell viability of DPSCs, were also analyzed. The influences of NFs on DPSCs neurogenesis were also analyzed. The results confirmed that an appropriate concentration of RGO promoted better DPSC neurogenesis. Furthermore, the use of random NFs facilitated contiguous junctions of differentiated cells, whereas the use of aligned NFs facilitated an aligned junction of differentiated cells along the direction of NF alignments. Our findings showed that RGO-PCL NFs can be a useful tool for DPSC neurogenesis, which will help regeneration in neurodegenerative and neurodefective diseases. MDPI 2018-07-21 /pmc/articles/PMC6071115/ /pubmed/30037100 http://dx.doi.org/10.3390/nano8070554 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Seonwoo, Hoon
Jang, Kyung-Je
Lee, Dohyeon
Park, Sunho
Lee, Myungchul
Park, Sangbae
Lim, Ki-Taek
Kim, Jangho
Chung, Jong Hoon
Neurogenic Differentiation of Human Dental Pulp Stem Cells on Graphene-Polycaprolactone Hybrid Nanofibers
title Neurogenic Differentiation of Human Dental Pulp Stem Cells on Graphene-Polycaprolactone Hybrid Nanofibers
title_full Neurogenic Differentiation of Human Dental Pulp Stem Cells on Graphene-Polycaprolactone Hybrid Nanofibers
title_fullStr Neurogenic Differentiation of Human Dental Pulp Stem Cells on Graphene-Polycaprolactone Hybrid Nanofibers
title_full_unstemmed Neurogenic Differentiation of Human Dental Pulp Stem Cells on Graphene-Polycaprolactone Hybrid Nanofibers
title_short Neurogenic Differentiation of Human Dental Pulp Stem Cells on Graphene-Polycaprolactone Hybrid Nanofibers
title_sort neurogenic differentiation of human dental pulp stem cells on graphene-polycaprolactone hybrid nanofibers
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071115/
https://www.ncbi.nlm.nih.gov/pubmed/30037100
http://dx.doi.org/10.3390/nano8070554
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