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Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell Metastasis Through Activating MAPK Signaling Pathway on HUVECs
Photodynamic therapy is a clinically used, minimally invasive therapeutic procedure that involves the application of photosensitizers which can locate in target cells and so be irradiated at a corresponding wavelength. Laser light irradiation activation of photosensitizers generates free reactive ox...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071160/ https://www.ncbi.nlm.nih.gov/pubmed/30068242 http://dx.doi.org/10.1177/1533033818785512 |
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author | Chen, Qing Li, Kai-Ting Tian, Si Yu, Ting-He Yu, Le-Hu Lin, Hai-Dan Bai, Ding-Qun |
author_facet | Chen, Qing Li, Kai-Ting Tian, Si Yu, Ting-He Yu, Le-Hu Lin, Hai-Dan Bai, Ding-Qun |
author_sort | Chen, Qing |
collection | PubMed |
description | Photodynamic therapy is a clinically used, minimally invasive therapeutic procedure that involves the application of photosensitizers which can locate in target cells and so be irradiated at a corresponding wavelength. Laser light irradiation activation of photosensitizers generates free reactive oxygen species, which induces selective cytotoxic activity in target cells. Within recent years, aloe-emodin as a photosensitizer has been successfully applied in photodynamic therapy applications. Angiogenesis plays an important role in tumor growth and metastasis; thus, the development of a novel target treatment for angiogenesis is essential in order to improve treatment therapeutics for cancer treatment. An essential step in angiogenesis involves the formation of tube-like structures during matrix degradation, rearrangement, and apoptosis of endothelial cells. In the present study, we investigated the mechanisms of photocytotoxicity induced by aloe-emodin in human umbilical vein endothelial cells. Analysis of cell proliferation results noted a significant decrease in cultured cells which received various concentrations of aloe-emodin and photodynamic therapy–induced light doses. Additionally, mitochondrial mechanisms of apoptotic cell death were observed in aloe-emodin photodynamic therapy–treated cells, as tube formation assays noted angiogenesis suppression after treatment. The capacity of migration and invasion of human umbilical vein endothelial cells was measured using the transwell assay and demonstrated that aloe-emodin photodynamic therapy significantly inhibited the migration and invasion of human umbilical vein endothelial cells. The expression of p38, extracellular signal-regulated kinase, the c-Jun N-terminal kinases, and vascular endothelial growth factor suggested that the cellular metastasis was related to mitogen-activated protein kinase signal pathway. Furthermore, disorganization of F action cytoskeleton components was observed after aloe-emodin photodynamic therapy. Overall, the findings from this study suggest that aloe-emodin photodynamic therapy inhibited angiogenesis and cellular metastasis in human umbilical vein endothelial cells by activating the mitogen-activated protein kinase apoptotic signaling cell death pathway. |
format | Online Article Text |
id | pubmed-6071160 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-60711602018-08-06 Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell Metastasis Through Activating MAPK Signaling Pathway on HUVECs Chen, Qing Li, Kai-Ting Tian, Si Yu, Ting-He Yu, Le-Hu Lin, Hai-Dan Bai, Ding-Qun Technol Cancer Res Treat Original Article Photodynamic therapy is a clinically used, minimally invasive therapeutic procedure that involves the application of photosensitizers which can locate in target cells and so be irradiated at a corresponding wavelength. Laser light irradiation activation of photosensitizers generates free reactive oxygen species, which induces selective cytotoxic activity in target cells. Within recent years, aloe-emodin as a photosensitizer has been successfully applied in photodynamic therapy applications. Angiogenesis plays an important role in tumor growth and metastasis; thus, the development of a novel target treatment for angiogenesis is essential in order to improve treatment therapeutics for cancer treatment. An essential step in angiogenesis involves the formation of tube-like structures during matrix degradation, rearrangement, and apoptosis of endothelial cells. In the present study, we investigated the mechanisms of photocytotoxicity induced by aloe-emodin in human umbilical vein endothelial cells. Analysis of cell proliferation results noted a significant decrease in cultured cells which received various concentrations of aloe-emodin and photodynamic therapy–induced light doses. Additionally, mitochondrial mechanisms of apoptotic cell death were observed in aloe-emodin photodynamic therapy–treated cells, as tube formation assays noted angiogenesis suppression after treatment. The capacity of migration and invasion of human umbilical vein endothelial cells was measured using the transwell assay and demonstrated that aloe-emodin photodynamic therapy significantly inhibited the migration and invasion of human umbilical vein endothelial cells. The expression of p38, extracellular signal-regulated kinase, the c-Jun N-terminal kinases, and vascular endothelial growth factor suggested that the cellular metastasis was related to mitogen-activated protein kinase signal pathway. Furthermore, disorganization of F action cytoskeleton components was observed after aloe-emodin photodynamic therapy. Overall, the findings from this study suggest that aloe-emodin photodynamic therapy inhibited angiogenesis and cellular metastasis in human umbilical vein endothelial cells by activating the mitogen-activated protein kinase apoptotic signaling cell death pathway. SAGE Publications 2018-08-01 /pmc/articles/PMC6071160/ /pubmed/30068242 http://dx.doi.org/10.1177/1533033818785512 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Chen, Qing Li, Kai-Ting Tian, Si Yu, Ting-He Yu, Le-Hu Lin, Hai-Dan Bai, Ding-Qun Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell Metastasis Through Activating MAPK Signaling Pathway on HUVECs |
title | Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell
Metastasis Through Activating MAPK Signaling Pathway on HUVECs |
title_full | Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell
Metastasis Through Activating MAPK Signaling Pathway on HUVECs |
title_fullStr | Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell
Metastasis Through Activating MAPK Signaling Pathway on HUVECs |
title_full_unstemmed | Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell
Metastasis Through Activating MAPK Signaling Pathway on HUVECs |
title_short | Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell
Metastasis Through Activating MAPK Signaling Pathway on HUVECs |
title_sort | photodynamic therapy mediated by aloe-emodin inhibited angiogenesis and cell
metastasis through activating mapk signaling pathway on huvecs |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071160/ https://www.ncbi.nlm.nih.gov/pubmed/30068242 http://dx.doi.org/10.1177/1533033818785512 |
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