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Dengue Virus Induces the Release of sCD40L and Changes in Levels of Membranal CD42b and CD40L Molecules in Human Platelets

Platelets are considered as significant players in innate and adaptive immune responses. The adhesion molecules they express, including P-selectin, CD40L, and CD42b, facilitate interactions with many cellular effectors. Upon interacting with a pathogen, platelets rapidly express and enhance their ad...

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Autores principales: Núñez-Avellaneda, Daniel, Mosso-Pani, Manuel Alejandro, Sánchez-Torres, Luvia E., Castro-Mussot, María Eugenia, Corona-de la Peña, Norma Angélica, Salazar, Ma. Isabel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071282/
https://www.ncbi.nlm.nih.gov/pubmed/29976871
http://dx.doi.org/10.3390/v10070357
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author Núñez-Avellaneda, Daniel
Mosso-Pani, Manuel Alejandro
Sánchez-Torres, Luvia E.
Castro-Mussot, María Eugenia
Corona-de la Peña, Norma Angélica
Salazar, Ma. Isabel
author_facet Núñez-Avellaneda, Daniel
Mosso-Pani, Manuel Alejandro
Sánchez-Torres, Luvia E.
Castro-Mussot, María Eugenia
Corona-de la Peña, Norma Angélica
Salazar, Ma. Isabel
author_sort Núñez-Avellaneda, Daniel
collection PubMed
description Platelets are considered as significant players in innate and adaptive immune responses. The adhesion molecules they express, including P-selectin, CD40L, and CD42b, facilitate interactions with many cellular effectors. Upon interacting with a pathogen, platelets rapidly express and enhance their adhesion molecules, and secrete cytokines and chemokines. A similar phenomenon occurs after exposure of platelets to thrombin, an agonist extensively used for in vitro activation of these cells. It was recently reported that the dengue virus not only interacts with platelets but possibly infects them, which triggers an increased expression of adhesion molecule P-selectin as well as secretion of IL-1β. In the present study, surface molecules of platelets like CD40L, CD42b, CD62P, and MHC class I were evaluated at 4 h of interaction with dengue virus serotype 2 (DENV-2), finding that DENV-2 induced a sharp rise in the membrane expression of all these molecules. At 2 and 4 h of DENV-2 stimulation of platelets, a significantly greater secretion of soluble CD40L (sCD40L) was found (versus basal levels) as well as cytokines such as GM-CSF, IL-6, IL-8, IL-10, and TNF-α. Compared to basal, DENV-2 elicited more than two-fold increase in these cytokines. Compared to the thrombin-induced response, the level generated by DENV-2 was much higher for GM-CSF, IL-6, and TNF-α. All these events induced by DENV end up in conspicuous morphological changes observed in platelets by confocal microscopy and transmission electron microscopy, very different from those elicited by thrombin in a more physiological scenery.
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spelling pubmed-60712822018-08-09 Dengue Virus Induces the Release of sCD40L and Changes in Levels of Membranal CD42b and CD40L Molecules in Human Platelets Núñez-Avellaneda, Daniel Mosso-Pani, Manuel Alejandro Sánchez-Torres, Luvia E. Castro-Mussot, María Eugenia Corona-de la Peña, Norma Angélica Salazar, Ma. Isabel Viruses Article Platelets are considered as significant players in innate and adaptive immune responses. The adhesion molecules they express, including P-selectin, CD40L, and CD42b, facilitate interactions with many cellular effectors. Upon interacting with a pathogen, platelets rapidly express and enhance their adhesion molecules, and secrete cytokines and chemokines. A similar phenomenon occurs after exposure of platelets to thrombin, an agonist extensively used for in vitro activation of these cells. It was recently reported that the dengue virus not only interacts with platelets but possibly infects them, which triggers an increased expression of adhesion molecule P-selectin as well as secretion of IL-1β. In the present study, surface molecules of platelets like CD40L, CD42b, CD62P, and MHC class I were evaluated at 4 h of interaction with dengue virus serotype 2 (DENV-2), finding that DENV-2 induced a sharp rise in the membrane expression of all these molecules. At 2 and 4 h of DENV-2 stimulation of platelets, a significantly greater secretion of soluble CD40L (sCD40L) was found (versus basal levels) as well as cytokines such as GM-CSF, IL-6, IL-8, IL-10, and TNF-α. Compared to basal, DENV-2 elicited more than two-fold increase in these cytokines. Compared to the thrombin-induced response, the level generated by DENV-2 was much higher for GM-CSF, IL-6, and TNF-α. All these events induced by DENV end up in conspicuous morphological changes observed in platelets by confocal microscopy and transmission electron microscopy, very different from those elicited by thrombin in a more physiological scenery. MDPI 2018-07-05 /pmc/articles/PMC6071282/ /pubmed/29976871 http://dx.doi.org/10.3390/v10070357 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Núñez-Avellaneda, Daniel
Mosso-Pani, Manuel Alejandro
Sánchez-Torres, Luvia E.
Castro-Mussot, María Eugenia
Corona-de la Peña, Norma Angélica
Salazar, Ma. Isabel
Dengue Virus Induces the Release of sCD40L and Changes in Levels of Membranal CD42b and CD40L Molecules in Human Platelets
title Dengue Virus Induces the Release of sCD40L and Changes in Levels of Membranal CD42b and CD40L Molecules in Human Platelets
title_full Dengue Virus Induces the Release of sCD40L and Changes in Levels of Membranal CD42b and CD40L Molecules in Human Platelets
title_fullStr Dengue Virus Induces the Release of sCD40L and Changes in Levels of Membranal CD42b and CD40L Molecules in Human Platelets
title_full_unstemmed Dengue Virus Induces the Release of sCD40L and Changes in Levels of Membranal CD42b and CD40L Molecules in Human Platelets
title_short Dengue Virus Induces the Release of sCD40L and Changes in Levels of Membranal CD42b and CD40L Molecules in Human Platelets
title_sort dengue virus induces the release of scd40l and changes in levels of membranal cd42b and cd40l molecules in human platelets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071282/
https://www.ncbi.nlm.nih.gov/pubmed/29976871
http://dx.doi.org/10.3390/v10070357
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