Active PSF Shaping and Adaptive Optics Enable Volumetric Localization Microscopy through Brain Sections

Single Molecule Switching Nanoscopy (SMSN) beyond the coverslip surface poses significant challenges. The sample-induced aberrations distort and blur single-molecule emission patterns. Combining active point-spread function shaping and efficient adaptive optics enables robust 3D-SMSN imaging at larg...

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Detalles Bibliográficos
Autores principales: Mlodzianoski, Michael J., Cheng-Hathaway, Paul J., Bemiller, Shane M., McCray, Tyler J., Liu, Sheng, Miller, David A., Lamb, Bruce T., Landreth, Gary E., Huang, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071422/
https://www.ncbi.nlm.nih.gov/pubmed/30013047
http://dx.doi.org/10.1038/s41592-018-0053-8
Descripción
Sumario:Single Molecule Switching Nanoscopy (SMSN) beyond the coverslip surface poses significant challenges. The sample-induced aberrations distort and blur single-molecule emission patterns. Combining active point-spread function shaping and efficient adaptive optics enables robust 3D-SMSN imaging at large depths. This development allowed us to image through 30-μm thick brain sections, visualize the morphology and the nanoscale details of amyloid-β filaments in a mouse model of Alzheimer’s disease and reconstruct their volumetric arrangements.

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