Active PSF Shaping and Adaptive Optics Enable Volumetric Localization Microscopy through Brain Sections

Single Molecule Switching Nanoscopy (SMSN) beyond the coverslip surface poses significant challenges. The sample-induced aberrations distort and blur single-molecule emission patterns. Combining active point-spread function shaping and efficient adaptive optics enables robust 3D-SMSN imaging at larg...

Descripción completa

Detalles Bibliográficos
Autores principales: Mlodzianoski, Michael J., Cheng-Hathaway, Paul J., Bemiller, Shane M., McCray, Tyler J., Liu, Sheng, Miller, David A., Lamb, Bruce T., Landreth, Gary E., Huang, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071422/
https://www.ncbi.nlm.nih.gov/pubmed/30013047
http://dx.doi.org/10.1038/s41592-018-0053-8
_version_ 1783343869235036160
author Mlodzianoski, Michael J.
Cheng-Hathaway, Paul J.
Bemiller, Shane M.
McCray, Tyler J.
Liu, Sheng
Miller, David A.
Lamb, Bruce T.
Landreth, Gary E.
Huang, Fang
author_facet Mlodzianoski, Michael J.
Cheng-Hathaway, Paul J.
Bemiller, Shane M.
McCray, Tyler J.
Liu, Sheng
Miller, David A.
Lamb, Bruce T.
Landreth, Gary E.
Huang, Fang
author_sort Mlodzianoski, Michael J.
collection PubMed
description Single Molecule Switching Nanoscopy (SMSN) beyond the coverslip surface poses significant challenges. The sample-induced aberrations distort and blur single-molecule emission patterns. Combining active point-spread function shaping and efficient adaptive optics enables robust 3D-SMSN imaging at large depths. This development allowed us to image through 30-μm thick brain sections, visualize the morphology and the nanoscale details of amyloid-β filaments in a mouse model of Alzheimer’s disease and reconstruct their volumetric arrangements.
format Online
Article
Text
id pubmed-6071422
institution National Center for Biotechnology Information
language English
publishDate 2018
record_format MEDLINE/PubMed
spelling pubmed-60714222019-01-16 Active PSF Shaping and Adaptive Optics Enable Volumetric Localization Microscopy through Brain Sections Mlodzianoski, Michael J. Cheng-Hathaway, Paul J. Bemiller, Shane M. McCray, Tyler J. Liu, Sheng Miller, David A. Lamb, Bruce T. Landreth, Gary E. Huang, Fang Nat Methods Article Single Molecule Switching Nanoscopy (SMSN) beyond the coverslip surface poses significant challenges. The sample-induced aberrations distort and blur single-molecule emission patterns. Combining active point-spread function shaping and efficient adaptive optics enables robust 3D-SMSN imaging at large depths. This development allowed us to image through 30-μm thick brain sections, visualize the morphology and the nanoscale details of amyloid-β filaments in a mouse model of Alzheimer’s disease and reconstruct their volumetric arrangements. 2018-07-16 2018-08 /pmc/articles/PMC6071422/ /pubmed/30013047 http://dx.doi.org/10.1038/s41592-018-0053-8 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Mlodzianoski, Michael J.
Cheng-Hathaway, Paul J.
Bemiller, Shane M.
McCray, Tyler J.
Liu, Sheng
Miller, David A.
Lamb, Bruce T.
Landreth, Gary E.
Huang, Fang
Active PSF Shaping and Adaptive Optics Enable Volumetric Localization Microscopy through Brain Sections
title Active PSF Shaping and Adaptive Optics Enable Volumetric Localization Microscopy through Brain Sections
title_full Active PSF Shaping and Adaptive Optics Enable Volumetric Localization Microscopy through Brain Sections
title_fullStr Active PSF Shaping and Adaptive Optics Enable Volumetric Localization Microscopy through Brain Sections
title_full_unstemmed Active PSF Shaping and Adaptive Optics Enable Volumetric Localization Microscopy through Brain Sections
title_short Active PSF Shaping and Adaptive Optics Enable Volumetric Localization Microscopy through Brain Sections
title_sort active psf shaping and adaptive optics enable volumetric localization microscopy through brain sections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071422/
https://www.ncbi.nlm.nih.gov/pubmed/30013047
http://dx.doi.org/10.1038/s41592-018-0053-8
work_keys_str_mv AT mlodzianoskimichaelj activepsfshapingandadaptiveopticsenablevolumetriclocalizationmicroscopythroughbrainsections
AT chenghathawaypaulj activepsfshapingandadaptiveopticsenablevolumetriclocalizationmicroscopythroughbrainsections
AT bemillershanem activepsfshapingandadaptiveopticsenablevolumetriclocalizationmicroscopythroughbrainsections
AT mccraytylerj activepsfshapingandadaptiveopticsenablevolumetriclocalizationmicroscopythroughbrainsections
AT liusheng activepsfshapingandadaptiveopticsenablevolumetriclocalizationmicroscopythroughbrainsections
AT millerdavida activepsfshapingandadaptiveopticsenablevolumetriclocalizationmicroscopythroughbrainsections
AT lambbrucet activepsfshapingandadaptiveopticsenablevolumetriclocalizationmicroscopythroughbrainsections
AT landrethgarye activepsfshapingandadaptiveopticsenablevolumetriclocalizationmicroscopythroughbrainsections
AT huangfang activepsfshapingandadaptiveopticsenablevolumetriclocalizationmicroscopythroughbrainsections

Ejemplares similares