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Genome-wide analysis reveals four key transcription factors associated with cadmium stress in creeping bentgrass (Agrostis stolonifera L.)

Cadmium (Cd) toxicity seriously affects the growth and development of plants, so studies on uptake, translocation, and accumulation of Cd in plants are crucial for phytoremediation. However, the molecular mechanism of the plant response to Cd stress remains poorly understood. The main objective of t...

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Autores principales: Yuan, Jianbo, Bai, Yuqing, Chao, Yuehui, Sun, Xinbo, He, Chunyan, Liang, Xiaohong, Xie, Lijuan, Han, Liebao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071620/
https://www.ncbi.nlm.nih.gov/pubmed/30083437
http://dx.doi.org/10.7717/peerj.5191
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author Yuan, Jianbo
Bai, Yuqing
Chao, Yuehui
Sun, Xinbo
He, Chunyan
Liang, Xiaohong
Xie, Lijuan
Han, Liebao
author_facet Yuan, Jianbo
Bai, Yuqing
Chao, Yuehui
Sun, Xinbo
He, Chunyan
Liang, Xiaohong
Xie, Lijuan
Han, Liebao
author_sort Yuan, Jianbo
collection PubMed
description Cadmium (Cd) toxicity seriously affects the growth and development of plants, so studies on uptake, translocation, and accumulation of Cd in plants are crucial for phytoremediation. However, the molecular mechanism of the plant response to Cd stress remains poorly understood. The main objective of this study was to reveal differentially expressed genes (DEGs) under lower (BT2_5) and higher (BT43) Cd concentration treatments in creeping bentgrass. A total of 463,184 unigenes were obtained from creeping bentgrass leaves using RNA sequencing technology. Observation of leaf tissue morphology showed that the higher Cd concentration damages leaf tissues. Four key transcription factor (TF) families, WRKY, bZIP, ERF, and MYB, are associated with Cd stress in creeping bentgrass. Our findings revealed that these four TFs play crucial roles during the creeping bentgrass response to Cd stress. This study is mainly focused on the molecular characteristics of DEGs under Cd stress using transcriptomic analysis in creeping bentgrass. These results provide novel insight into the regulatory mechanisms of respond to Cd stress and enrich information for phytoremediation.
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spelling pubmed-60716202018-08-06 Genome-wide analysis reveals four key transcription factors associated with cadmium stress in creeping bentgrass (Agrostis stolonifera L.) Yuan, Jianbo Bai, Yuqing Chao, Yuehui Sun, Xinbo He, Chunyan Liang, Xiaohong Xie, Lijuan Han, Liebao PeerJ Bioinformatics Cadmium (Cd) toxicity seriously affects the growth and development of plants, so studies on uptake, translocation, and accumulation of Cd in plants are crucial for phytoremediation. However, the molecular mechanism of the plant response to Cd stress remains poorly understood. The main objective of this study was to reveal differentially expressed genes (DEGs) under lower (BT2_5) and higher (BT43) Cd concentration treatments in creeping bentgrass. A total of 463,184 unigenes were obtained from creeping bentgrass leaves using RNA sequencing technology. Observation of leaf tissue morphology showed that the higher Cd concentration damages leaf tissues. Four key transcription factor (TF) families, WRKY, bZIP, ERF, and MYB, are associated with Cd stress in creeping bentgrass. Our findings revealed that these four TFs play crucial roles during the creeping bentgrass response to Cd stress. This study is mainly focused on the molecular characteristics of DEGs under Cd stress using transcriptomic analysis in creeping bentgrass. These results provide novel insight into the regulatory mechanisms of respond to Cd stress and enrich information for phytoremediation. PeerJ Inc. 2018-07-30 /pmc/articles/PMC6071620/ /pubmed/30083437 http://dx.doi.org/10.7717/peerj.5191 Text en ©2018 Yuan et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioinformatics
Yuan, Jianbo
Bai, Yuqing
Chao, Yuehui
Sun, Xinbo
He, Chunyan
Liang, Xiaohong
Xie, Lijuan
Han, Liebao
Genome-wide analysis reveals four key transcription factors associated with cadmium stress in creeping bentgrass (Agrostis stolonifera L.)
title Genome-wide analysis reveals four key transcription factors associated with cadmium stress in creeping bentgrass (Agrostis stolonifera L.)
title_full Genome-wide analysis reveals four key transcription factors associated with cadmium stress in creeping bentgrass (Agrostis stolonifera L.)
title_fullStr Genome-wide analysis reveals four key transcription factors associated with cadmium stress in creeping bentgrass (Agrostis stolonifera L.)
title_full_unstemmed Genome-wide analysis reveals four key transcription factors associated with cadmium stress in creeping bentgrass (Agrostis stolonifera L.)
title_short Genome-wide analysis reveals four key transcription factors associated with cadmium stress in creeping bentgrass (Agrostis stolonifera L.)
title_sort genome-wide analysis reveals four key transcription factors associated with cadmium stress in creeping bentgrass (agrostis stolonifera l.)
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071620/
https://www.ncbi.nlm.nih.gov/pubmed/30083437
http://dx.doi.org/10.7717/peerj.5191
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