Cargando…
Strong binding and fluorescence sensing of bisphosphonates by guanidinium-modified calix[5]arene
Based on the indicator displacement assay (IDA) approach, we herein report the fluorescence “switch-on” sensing and quantitative detection of bisphosphonates (BPs), a class of drugs extensively used in the treatment of patients with various skeletal diseases. Guanidinium-modified calix[5]arene (GC5A...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Beilstein-Institut
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071700/ https://www.ncbi.nlm.nih.gov/pubmed/30112088 http://dx.doi.org/10.3762/bjoc.14.157 |
_version_ | 1783343914405593088 |
---|---|
author | Gao, Jie Zheng, Zhe Shi, Lin Wu, Si-Qi Sun, Hongwei Guo, Dong-Sheng |
author_facet | Gao, Jie Zheng, Zhe Shi, Lin Wu, Si-Qi Sun, Hongwei Guo, Dong-Sheng |
author_sort | Gao, Jie |
collection | PubMed |
description | Based on the indicator displacement assay (IDA) approach, we herein report the fluorescence “switch-on” sensing and quantitative detection of bisphosphonates (BPs), a class of drugs extensively used in the treatment of patients with various skeletal diseases. Guanidinium-modified calix[5]arene (GC5A) affords strong binding on the micromolar to nanomolar level towards BPs dominantly via multiple salt bridge interactions, which was evaluated by fluorescence competitive titrations. Fluorescent IDA enables the highly sensitive and label-free detection of BPs in buffer solution, and more importantly, in artificial urine. Calibration lines were therefore set up in untreated artificial urine, allowing for quantifying the concentrations of BPs in the biologically relevant low range. |
format | Online Article Text |
id | pubmed-6071700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Beilstein-Institut |
record_format | MEDLINE/PubMed |
spelling | pubmed-60717002018-08-15 Strong binding and fluorescence sensing of bisphosphonates by guanidinium-modified calix[5]arene Gao, Jie Zheng, Zhe Shi, Lin Wu, Si-Qi Sun, Hongwei Guo, Dong-Sheng Beilstein J Org Chem Full Research Paper Based on the indicator displacement assay (IDA) approach, we herein report the fluorescence “switch-on” sensing and quantitative detection of bisphosphonates (BPs), a class of drugs extensively used in the treatment of patients with various skeletal diseases. Guanidinium-modified calix[5]arene (GC5A) affords strong binding on the micromolar to nanomolar level towards BPs dominantly via multiple salt bridge interactions, which was evaluated by fluorescence competitive titrations. Fluorescent IDA enables the highly sensitive and label-free detection of BPs in buffer solution, and more importantly, in artificial urine. Calibration lines were therefore set up in untreated artificial urine, allowing for quantifying the concentrations of BPs in the biologically relevant low range. Beilstein-Institut 2018-07-19 /pmc/articles/PMC6071700/ /pubmed/30112088 http://dx.doi.org/10.3762/bjoc.14.157 Text en Copyright © 2018, Gao et al. https://creativecommons.org/licenses/by/4.0https://www.beilstein-journals.org/bjoc/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0). Please note that the reuse, redistribution and reproduction in particular requires that the authors and source are credited. The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc/terms) |
spellingShingle | Full Research Paper Gao, Jie Zheng, Zhe Shi, Lin Wu, Si-Qi Sun, Hongwei Guo, Dong-Sheng Strong binding and fluorescence sensing of bisphosphonates by guanidinium-modified calix[5]arene |
title | Strong binding and fluorescence sensing of bisphosphonates by guanidinium-modified calix[5]arene |
title_full | Strong binding and fluorescence sensing of bisphosphonates by guanidinium-modified calix[5]arene |
title_fullStr | Strong binding and fluorescence sensing of bisphosphonates by guanidinium-modified calix[5]arene |
title_full_unstemmed | Strong binding and fluorescence sensing of bisphosphonates by guanidinium-modified calix[5]arene |
title_short | Strong binding and fluorescence sensing of bisphosphonates by guanidinium-modified calix[5]arene |
title_sort | strong binding and fluorescence sensing of bisphosphonates by guanidinium-modified calix[5]arene |
topic | Full Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071700/ https://www.ncbi.nlm.nih.gov/pubmed/30112088 http://dx.doi.org/10.3762/bjoc.14.157 |
work_keys_str_mv | AT gaojie strongbindingandfluorescencesensingofbisphosphonatesbyguanidiniummodifiedcalix5arene AT zhengzhe strongbindingandfluorescencesensingofbisphosphonatesbyguanidiniummodifiedcalix5arene AT shilin strongbindingandfluorescencesensingofbisphosphonatesbyguanidiniummodifiedcalix5arene AT wusiqi strongbindingandfluorescencesensingofbisphosphonatesbyguanidiniummodifiedcalix5arene AT sunhongwei strongbindingandfluorescencesensingofbisphosphonatesbyguanidiniummodifiedcalix5arene AT guodongsheng strongbindingandfluorescencesensingofbisphosphonatesbyguanidiniummodifiedcalix5arene |