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Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis
Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis is a highly regulated process, but the detailed mechanism still remains inc...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Biochemistry and Molecular Biology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071775/ https://www.ncbi.nlm.nih.gov/pubmed/29895700 http://dx.doi.org/10.1194/jlr.M083899 |
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author | Chung, Gary Hong Chun Domart, Marie-Charlotte Peddie, Christopher Mantell, Judith Mclaverty, Kieran Arabiotorre, Angela Hodgson, Lorna Byrne, Richard D. Verkade, Paul Arkill, Kenton Collinson, Lucy M. Larijani, Banafshé |
author_facet | Chung, Gary Hong Chun Domart, Marie-Charlotte Peddie, Christopher Mantell, Judith Mclaverty, Kieran Arabiotorre, Angela Hodgson, Lorna Byrne, Richard D. Verkade, Paul Arkill, Kenton Collinson, Lucy M. Larijani, Banafshé |
author_sort | Chung, Gary Hong Chun |
collection | PubMed |
description | Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis is a highly regulated process, but the detailed mechanism still remains incompletely understood. Previous studies have found that isolated diacylglycerol (DAG)-containing vesicles are essential for completing the fusion of the NE in nonsomatic cells. We investigated the impact of DAG depletion from the cis-Golgi in mammalian cells on NE reassembly. Using advanced electron microscopy, we observed an enriched DAG population of vesicles at the vicinity of the NE gaps of telophase mammalian cells. We applied a mini singlet oxygen generator-C1-domain tag that localized DAG-enriched vesicles at the perinuclear region, which suggested the existence of NE fusogenic vesicles. We quantified the impact of Golgi-DAG depletion by measuring the in situ NE rim curvature of the reforming NE. The rim curvature in these cells was significantly reduced compared with controls, which indicated a localized defect in NE morphology. Our novel results demonstrate the significance of the role of DAG from the cis-Golgi for the regulation of NE assembly. |
format | Online Article Text |
id | pubmed-6071775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-60717752018-08-06 Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis Chung, Gary Hong Chun Domart, Marie-Charlotte Peddie, Christopher Mantell, Judith Mclaverty, Kieran Arabiotorre, Angela Hodgson, Lorna Byrne, Richard D. Verkade, Paul Arkill, Kenton Collinson, Lucy M. Larijani, Banafshé J Lipid Res Research Articles Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis is a highly regulated process, but the detailed mechanism still remains incompletely understood. Previous studies have found that isolated diacylglycerol (DAG)-containing vesicles are essential for completing the fusion of the NE in nonsomatic cells. We investigated the impact of DAG depletion from the cis-Golgi in mammalian cells on NE reassembly. Using advanced electron microscopy, we observed an enriched DAG population of vesicles at the vicinity of the NE gaps of telophase mammalian cells. We applied a mini singlet oxygen generator-C1-domain tag that localized DAG-enriched vesicles at the perinuclear region, which suggested the existence of NE fusogenic vesicles. We quantified the impact of Golgi-DAG depletion by measuring the in situ NE rim curvature of the reforming NE. The rim curvature in these cells was significantly reduced compared with controls, which indicated a localized defect in NE morphology. Our novel results demonstrate the significance of the role of DAG from the cis-Golgi for the regulation of NE assembly. The American Society for Biochemistry and Molecular Biology 2018-08 2018-06-12 /pmc/articles/PMC6071775/ /pubmed/29895700 http://dx.doi.org/10.1194/jlr.M083899 Text en Copyright © 2018 Chung et al. Published by The American Society for Biochemistry and Molecular Biology, Inc. http://creativecommons.org/licenses/by/4.0/ Author’s Choice—Final version open access under the terms of the Creative Commons CC-BY license. |
spellingShingle | Research Articles Chung, Gary Hong Chun Domart, Marie-Charlotte Peddie, Christopher Mantell, Judith Mclaverty, Kieran Arabiotorre, Angela Hodgson, Lorna Byrne, Richard D. Verkade, Paul Arkill, Kenton Collinson, Lucy M. Larijani, Banafshé Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis |
title | Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis |
title_full | Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis |
title_fullStr | Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis |
title_full_unstemmed | Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis |
title_short | Acute depletion of diacylglycerol from the cis-Golgi affects localized nuclear envelope morphology during mitosis |
title_sort | acute depletion of diacylglycerol from the cis-golgi affects localized nuclear envelope morphology during mitosis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071775/ https://www.ncbi.nlm.nih.gov/pubmed/29895700 http://dx.doi.org/10.1194/jlr.M083899 |
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