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Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior

Mouse lines selectively bred for high voluntary wheel-running behavior are helpful models for uncovering gene networks associated with increased motivation for physical activity and other reward-dependent behaviors. The fact that multiple brain regions are hypothesized to contribute to distinct beha...

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Autores principales: Zhang, Pan, Rhodes, Justin S., Garland, Theodore, Perez, Sam D., Southey, Bruce R., Rodriguez-Zas, Sandra L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072066/
https://www.ncbi.nlm.nih.gov/pubmed/30071007
http://dx.doi.org/10.1371/journal.pone.0201773
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author Zhang, Pan
Rhodes, Justin S.
Garland, Theodore
Perez, Sam D.
Southey, Bruce R.
Rodriguez-Zas, Sandra L.
author_facet Zhang, Pan
Rhodes, Justin S.
Garland, Theodore
Perez, Sam D.
Southey, Bruce R.
Rodriguez-Zas, Sandra L.
author_sort Zhang, Pan
collection PubMed
description Mouse lines selectively bred for high voluntary wheel-running behavior are helpful models for uncovering gene networks associated with increased motivation for physical activity and other reward-dependent behaviors. The fact that multiple brain regions are hypothesized to contribute to distinct behavior components necessitates the simultaneous study of these regions. The goals of this study were to identify brain-region dependent and independent gene expression patterns, regulators, and networks associated with increased voluntary wheel-running behavior. The cerebellum and striatum from a high voluntary running line and a non-selected control line were compared. Neuropeptide genes annotated to reward-dependent processes including neuropeptide S receptor 1 (Npsr1), neuropeptide Y (Npy), and proprotein convertase subtilisin/kexin type 9 (Pcsk9), and genes implicated in motor coordination including vitamin D receptor (Vdr) and keratin, type I cytoskeletal 25 (Krt25) were among the genes exhibiting activity line-by-region interaction effects. Genes annotated to the Parkinson pathway presented consistent line patterns, albeit at different orders of magnitude between brain regions, suggesting some parallel events in response to selection for high voluntary activity. The comparison of gene networks between brain regions highlighted genes including transcription factor AP-2-delta (Tfap2d), distal-less homeobox 5 gene (Dlx5) and sine oculis homeobox homolog 3 (Six3) that exhibited line differential expression in one brain region and are associated with reward-dependent behaviors. Transcription factors including En2, Stat6 and Eomes predominated among regulators of genes that differed in expression between lines. Results from the simultaneous study of striatum and cerebellum confirm the necessity to study molecular mechanisms associated with voluntary activity and reward-dependent behaviors in consideration of brain region dependencies.
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spelling pubmed-60720662018-08-16 Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior Zhang, Pan Rhodes, Justin S. Garland, Theodore Perez, Sam D. Southey, Bruce R. Rodriguez-Zas, Sandra L. PLoS One Research Article Mouse lines selectively bred for high voluntary wheel-running behavior are helpful models for uncovering gene networks associated with increased motivation for physical activity and other reward-dependent behaviors. The fact that multiple brain regions are hypothesized to contribute to distinct behavior components necessitates the simultaneous study of these regions. The goals of this study were to identify brain-region dependent and independent gene expression patterns, regulators, and networks associated with increased voluntary wheel-running behavior. The cerebellum and striatum from a high voluntary running line and a non-selected control line were compared. Neuropeptide genes annotated to reward-dependent processes including neuropeptide S receptor 1 (Npsr1), neuropeptide Y (Npy), and proprotein convertase subtilisin/kexin type 9 (Pcsk9), and genes implicated in motor coordination including vitamin D receptor (Vdr) and keratin, type I cytoskeletal 25 (Krt25) were among the genes exhibiting activity line-by-region interaction effects. Genes annotated to the Parkinson pathway presented consistent line patterns, albeit at different orders of magnitude between brain regions, suggesting some parallel events in response to selection for high voluntary activity. The comparison of gene networks between brain regions highlighted genes including transcription factor AP-2-delta (Tfap2d), distal-less homeobox 5 gene (Dlx5) and sine oculis homeobox homolog 3 (Six3) that exhibited line differential expression in one brain region and are associated with reward-dependent behaviors. Transcription factors including En2, Stat6 and Eomes predominated among regulators of genes that differed in expression between lines. Results from the simultaneous study of striatum and cerebellum confirm the necessity to study molecular mechanisms associated with voluntary activity and reward-dependent behaviors in consideration of brain region dependencies. Public Library of Science 2018-08-02 /pmc/articles/PMC6072066/ /pubmed/30071007 http://dx.doi.org/10.1371/journal.pone.0201773 Text en © 2018 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhang, Pan
Rhodes, Justin S.
Garland, Theodore
Perez, Sam D.
Southey, Bruce R.
Rodriguez-Zas, Sandra L.
Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior
title Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior
title_full Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior
title_fullStr Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior
title_full_unstemmed Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior
title_short Brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior
title_sort brain region-dependent gene networks associated with selective breeding for increased voluntary wheel-running behavior
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072066/
https://www.ncbi.nlm.nih.gov/pubmed/30071007
http://dx.doi.org/10.1371/journal.pone.0201773
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