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Cardiovascular effects of H(3) histamine receptor inverse agonist/ H(4) histamine receptor agonist, clobenpropit, in hemorrhage-shocked rats

Hemorrhagic shock has a potential to be life-threatening when it is not treated. The main causes of hemorrhagic shock involve: (1) forces causing injury; and (2) diseases that can cause hemorrhage., Therefore, due to the causes of hemorrhagic shock and the life-threatening potential, the search for...

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Autores principales: Wanot, Bartosz, Jasikowska, Karolina, Niewiadomska, Ewa, Biskupek-Wanot, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072086/
https://www.ncbi.nlm.nih.gov/pubmed/30071054
http://dx.doi.org/10.1371/journal.pone.0201519
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author Wanot, Bartosz
Jasikowska, Karolina
Niewiadomska, Ewa
Biskupek-Wanot, Agnieszka
author_facet Wanot, Bartosz
Jasikowska, Karolina
Niewiadomska, Ewa
Biskupek-Wanot, Agnieszka
author_sort Wanot, Bartosz
collection PubMed
description Hemorrhagic shock has a potential to be life-threatening when it is not treated. The main causes of hemorrhagic shock involve: (1) forces causing injury; and (2) diseases that can cause hemorrhage., Therefore, due to the causes of hemorrhagic shock and the life-threatening potential, the search for new methods of shock treatment is extremely valuable to the modern medicine. The aim of this study was to investigate the influence of clobenpropit in the model of hemorrhagic shock. The experiments were conducted in 110 adult male Wistar rats weighing between 205 and 470g. 1, 2 and 5 μmol/kg of intravenous H(3) receptors reverse agonists, clobentropit, and/or 1, 5 and 10 μmol/kg H(3) receptor agonist, imetit, were used as general anesthetics. Irreversible hemorrhagic shock was induced by the paused bleeding until the mean arterial pressure (MAP) lowered to the level of 20–25 mmHg. It was proved that, in cases of critical hypotension, clobenpropit triggered a dose-dependent increase of: systolic blood pressure (SBP), diastolic blood pressure (DBP), MPA and heart rate (HR) of rats with critical hypotension. The most significant changes in hemodynamic parameters were achieved by administrating dosages of 2 mmol/kg. This resulted in the survival rate increase to up to 100%. However, imetit did not trigger any hemodynamic changes nor an increase in SBP, DBP, MAP or HR. Furthermore, it was found that the premedication with prazosin, yohimbine, 6-hydroxydopamine and the vasopressin V(1a) receptor antagonist blocked the effects of clobenpropit. Additionally, premedication with propranolol, captopril and ZD 7155 did not cause any significant changes in the measured hemodynamic parameters. In conclusion, after an intravenous injection clobenpropit, the inverse agonist of H(3) histamine receptors/agonist of histamine receptors H(4), causes a resuscitating effect on rats in hemorrhagic shock. Moreover, such effect is based on the effector mechanisms of sympathetic nervous system and vasopressin.
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spelling pubmed-60720862018-08-16 Cardiovascular effects of H(3) histamine receptor inverse agonist/ H(4) histamine receptor agonist, clobenpropit, in hemorrhage-shocked rats Wanot, Bartosz Jasikowska, Karolina Niewiadomska, Ewa Biskupek-Wanot, Agnieszka PLoS One Research Article Hemorrhagic shock has a potential to be life-threatening when it is not treated. The main causes of hemorrhagic shock involve: (1) forces causing injury; and (2) diseases that can cause hemorrhage., Therefore, due to the causes of hemorrhagic shock and the life-threatening potential, the search for new methods of shock treatment is extremely valuable to the modern medicine. The aim of this study was to investigate the influence of clobenpropit in the model of hemorrhagic shock. The experiments were conducted in 110 adult male Wistar rats weighing between 205 and 470g. 1, 2 and 5 μmol/kg of intravenous H(3) receptors reverse agonists, clobentropit, and/or 1, 5 and 10 μmol/kg H(3) receptor agonist, imetit, were used as general anesthetics. Irreversible hemorrhagic shock was induced by the paused bleeding until the mean arterial pressure (MAP) lowered to the level of 20–25 mmHg. It was proved that, in cases of critical hypotension, clobenpropit triggered a dose-dependent increase of: systolic blood pressure (SBP), diastolic blood pressure (DBP), MPA and heart rate (HR) of rats with critical hypotension. The most significant changes in hemodynamic parameters were achieved by administrating dosages of 2 mmol/kg. This resulted in the survival rate increase to up to 100%. However, imetit did not trigger any hemodynamic changes nor an increase in SBP, DBP, MAP or HR. Furthermore, it was found that the premedication with prazosin, yohimbine, 6-hydroxydopamine and the vasopressin V(1a) receptor antagonist blocked the effects of clobenpropit. Additionally, premedication with propranolol, captopril and ZD 7155 did not cause any significant changes in the measured hemodynamic parameters. In conclusion, after an intravenous injection clobenpropit, the inverse agonist of H(3) histamine receptors/agonist of histamine receptors H(4), causes a resuscitating effect on rats in hemorrhagic shock. Moreover, such effect is based on the effector mechanisms of sympathetic nervous system and vasopressin. Public Library of Science 2018-08-02 /pmc/articles/PMC6072086/ /pubmed/30071054 http://dx.doi.org/10.1371/journal.pone.0201519 Text en © 2018 Wanot et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wanot, Bartosz
Jasikowska, Karolina
Niewiadomska, Ewa
Biskupek-Wanot, Agnieszka
Cardiovascular effects of H(3) histamine receptor inverse agonist/ H(4) histamine receptor agonist, clobenpropit, in hemorrhage-shocked rats
title Cardiovascular effects of H(3) histamine receptor inverse agonist/ H(4) histamine receptor agonist, clobenpropit, in hemorrhage-shocked rats
title_full Cardiovascular effects of H(3) histamine receptor inverse agonist/ H(4) histamine receptor agonist, clobenpropit, in hemorrhage-shocked rats
title_fullStr Cardiovascular effects of H(3) histamine receptor inverse agonist/ H(4) histamine receptor agonist, clobenpropit, in hemorrhage-shocked rats
title_full_unstemmed Cardiovascular effects of H(3) histamine receptor inverse agonist/ H(4) histamine receptor agonist, clobenpropit, in hemorrhage-shocked rats
title_short Cardiovascular effects of H(3) histamine receptor inverse agonist/ H(4) histamine receptor agonist, clobenpropit, in hemorrhage-shocked rats
title_sort cardiovascular effects of h(3) histamine receptor inverse agonist/ h(4) histamine receptor agonist, clobenpropit, in hemorrhage-shocked rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072086/
https://www.ncbi.nlm.nih.gov/pubmed/30071054
http://dx.doi.org/10.1371/journal.pone.0201519
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