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miR-339-5p negatively regulates loureirin A-induced hair follicle stem cell differentiation by targeting DLX5

Our previous study indicated that loureirin A induces hair follicle stem cell (HFSC) differentiation through Wnt/β-catenin signaling pathway activation. However, if and how microRNAs (miRNAs/miRs) modulate loureirin A-induced differentiation remains to be elucidated. In the present study, HFSCs were...

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Autores principales: Li, Xiangjun, Wu, Yuqiong, Xie, Fangfang, Zhang, Fengxue, Zhang, Saixia, Zhou, Jianhong, Chen, Dongfeng, Liu, Aijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072140/
https://www.ncbi.nlm.nih.gov/pubmed/29901112
http://dx.doi.org/10.3892/mmr.2018.9110
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author Li, Xiangjun
Wu, Yuqiong
Xie, Fangfang
Zhang, Fengxue
Zhang, Saixia
Zhou, Jianhong
Chen, Dongfeng
Liu, Aijun
author_facet Li, Xiangjun
Wu, Yuqiong
Xie, Fangfang
Zhang, Fengxue
Zhang, Saixia
Zhou, Jianhong
Chen, Dongfeng
Liu, Aijun
author_sort Li, Xiangjun
collection PubMed
description Our previous study indicated that loureirin A induces hair follicle stem cell (HFSC) differentiation through Wnt/β-catenin signaling pathway activation. However, if and how microRNAs (miRNAs/miRs) modulate loureirin A-induced differentiation remains to be elucidated. In the present study, HFSCs were separated from the vibrissae of rats and identified by CD34 and keratin, type 1 cytoskeletal (K)15 expression. Microarray-based miRNA profiling analysis revealed that miR-339-5p was downregulated in loureirin A-induced HFSC differentiation. miR-339-5p overexpression by transfection with miR-339-5p mimics markedly inhibited the expression of K10 and involucrin, which are markers of epidermal differentiation, whereas inhibition of miR-339-5p by miR-339-5p inhibitor transfection promoted the expression of K10 and involucrin. These results suggest that miR-339-5p is a negative regulator of HFSC differentiation following induction by loureirin A. These findings were confirmed by a luciferase assay. Homeobox protein DLX-5 (DLX5) was identified as a direct target of miR-339-5p. Furthermore, it was demonstrated that miR-339-5p inhibited DLX5. Overexpression of miR-339-5p by mimic transfection significantly inhibited protein Wnt-3a (Wnt3a) expression, while inhibition of miR-339-5p by inhibitor transfection significantly increased the expression of Wnt3a. Furthermore, small interfering RNA targeting DLX5 was transfected into HFSCs, and western blot analysis revealed that Wnt3a, involucrin and K10 expression was significantly downregulated. Taken together, these results suggest that miR-339-5p negatively regulated loureirin A-induced HFSC differentiation by targeting DLX5, resulting in Wnt/β-catenin signaling pathway inhibition. This may provide a possible therapeutic target for skin repair and regeneration.
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spelling pubmed-60721402018-08-06 miR-339-5p negatively regulates loureirin A-induced hair follicle stem cell differentiation by targeting DLX5 Li, Xiangjun Wu, Yuqiong Xie, Fangfang Zhang, Fengxue Zhang, Saixia Zhou, Jianhong Chen, Dongfeng Liu, Aijun Mol Med Rep Articles Our previous study indicated that loureirin A induces hair follicle stem cell (HFSC) differentiation through Wnt/β-catenin signaling pathway activation. However, if and how microRNAs (miRNAs/miRs) modulate loureirin A-induced differentiation remains to be elucidated. In the present study, HFSCs were separated from the vibrissae of rats and identified by CD34 and keratin, type 1 cytoskeletal (K)15 expression. Microarray-based miRNA profiling analysis revealed that miR-339-5p was downregulated in loureirin A-induced HFSC differentiation. miR-339-5p overexpression by transfection with miR-339-5p mimics markedly inhibited the expression of K10 and involucrin, which are markers of epidermal differentiation, whereas inhibition of miR-339-5p by miR-339-5p inhibitor transfection promoted the expression of K10 and involucrin. These results suggest that miR-339-5p is a negative regulator of HFSC differentiation following induction by loureirin A. These findings were confirmed by a luciferase assay. Homeobox protein DLX-5 (DLX5) was identified as a direct target of miR-339-5p. Furthermore, it was demonstrated that miR-339-5p inhibited DLX5. Overexpression of miR-339-5p by mimic transfection significantly inhibited protein Wnt-3a (Wnt3a) expression, while inhibition of miR-339-5p by inhibitor transfection significantly increased the expression of Wnt3a. Furthermore, small interfering RNA targeting DLX5 was transfected into HFSCs, and western blot analysis revealed that Wnt3a, involucrin and K10 expression was significantly downregulated. Taken together, these results suggest that miR-339-5p negatively regulated loureirin A-induced HFSC differentiation by targeting DLX5, resulting in Wnt/β-catenin signaling pathway inhibition. This may provide a possible therapeutic target for skin repair and regeneration. D.A. Spandidos 2018-08 2018-05-31 /pmc/articles/PMC6072140/ /pubmed/29901112 http://dx.doi.org/10.3892/mmr.2018.9110 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Xiangjun
Wu, Yuqiong
Xie, Fangfang
Zhang, Fengxue
Zhang, Saixia
Zhou, Jianhong
Chen, Dongfeng
Liu, Aijun
miR-339-5p negatively regulates loureirin A-induced hair follicle stem cell differentiation by targeting DLX5
title miR-339-5p negatively regulates loureirin A-induced hair follicle stem cell differentiation by targeting DLX5
title_full miR-339-5p negatively regulates loureirin A-induced hair follicle stem cell differentiation by targeting DLX5
title_fullStr miR-339-5p negatively regulates loureirin A-induced hair follicle stem cell differentiation by targeting DLX5
title_full_unstemmed miR-339-5p negatively regulates loureirin A-induced hair follicle stem cell differentiation by targeting DLX5
title_short miR-339-5p negatively regulates loureirin A-induced hair follicle stem cell differentiation by targeting DLX5
title_sort mir-339-5p negatively regulates loureirin a-induced hair follicle stem cell differentiation by targeting dlx5
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072140/
https://www.ncbi.nlm.nih.gov/pubmed/29901112
http://dx.doi.org/10.3892/mmr.2018.9110
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