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Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling
Long noncoding RNA AFAP1-AS1 has been shown to promote tumor progression in several human cancer types, such as thyroid cancer, tongue squamous cell carcinoma and lung cancer. However, the role of AFAP1-AS1 in osteosarcoma (OS) has not been investigated. In the present study, the expression of AFAP1...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072147/ https://www.ncbi.nlm.nih.gov/pubmed/29901121 http://dx.doi.org/10.3892/mmr.2018.9131 |
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author | Li, Rongrui Liu, Shichen Li, Yao Tang, Qingxi Xie, Yunchuan Zhai, Raosheng |
author_facet | Li, Rongrui Liu, Shichen Li, Yao Tang, Qingxi Xie, Yunchuan Zhai, Raosheng |
author_sort | Li, Rongrui |
collection | PubMed |
description | Long noncoding RNA AFAP1-AS1 has been shown to promote tumor progression in several human cancer types, such as thyroid cancer, tongue squamous cell carcinoma and lung cancer. However, the role of AFAP1-AS1 in osteosarcoma (OS) has not been investigated. In the present study, the expression of AFAP1-AS1 was significantly upregulated in OS tissues and cell lines. Moreover, AFAP1-AS1 expression was negatively correlated with OS patient prognosis. Besides, AFAP1-AS1 knockdown significantly inhibited the proliferation and invasion of OS cells in vitro. Furthermore, in vivo xenograft experiments indicated that AFAP1-AS1 depletion delayed tumor growth. Regarding the underlying mechanism, AFAP1-AS1 served as a sponge to repress the level of microRNA (miR)-4695-5p, which targeted transcription factor (TCF)4, a pivot effector of Wnt/β-catenin signaling pathway. It was demonstrated that overexpression of AFAP1-AS1 inhibited the expression of miR-4695-5p, while miR-4695-5p overexpression decreased TCF4 expression and reduced activation of Wnt/β-catenin pathway. Through rescue assays, it was demonstrated that restoration of TCF4 expression reversed the effects of AFAP1-AS1 knockdown or miR-4695-5p overexpression on OS cells. Taken together, these findings demonstrated that the AFAP1-AS1/miR-4695-5p/TCF4-β-catenin axis played an important role in OS progression. |
format | Online Article Text |
id | pubmed-6072147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-60721472018-08-06 Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling Li, Rongrui Liu, Shichen Li, Yao Tang, Qingxi Xie, Yunchuan Zhai, Raosheng Mol Med Rep Articles Long noncoding RNA AFAP1-AS1 has been shown to promote tumor progression in several human cancer types, such as thyroid cancer, tongue squamous cell carcinoma and lung cancer. However, the role of AFAP1-AS1 in osteosarcoma (OS) has not been investigated. In the present study, the expression of AFAP1-AS1 was significantly upregulated in OS tissues and cell lines. Moreover, AFAP1-AS1 expression was negatively correlated with OS patient prognosis. Besides, AFAP1-AS1 knockdown significantly inhibited the proliferation and invasion of OS cells in vitro. Furthermore, in vivo xenograft experiments indicated that AFAP1-AS1 depletion delayed tumor growth. Regarding the underlying mechanism, AFAP1-AS1 served as a sponge to repress the level of microRNA (miR)-4695-5p, which targeted transcription factor (TCF)4, a pivot effector of Wnt/β-catenin signaling pathway. It was demonstrated that overexpression of AFAP1-AS1 inhibited the expression of miR-4695-5p, while miR-4695-5p overexpression decreased TCF4 expression and reduced activation of Wnt/β-catenin pathway. Through rescue assays, it was demonstrated that restoration of TCF4 expression reversed the effects of AFAP1-AS1 knockdown or miR-4695-5p overexpression on OS cells. Taken together, these findings demonstrated that the AFAP1-AS1/miR-4695-5p/TCF4-β-catenin axis played an important role in OS progression. D.A. Spandidos 2018-08 2018-06-05 /pmc/articles/PMC6072147/ /pubmed/29901121 http://dx.doi.org/10.3892/mmr.2018.9131 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Rongrui Liu, Shichen Li, Yao Tang, Qingxi Xie, Yunchuan Zhai, Raosheng Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling |
title | Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling |
title_full | Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling |
title_fullStr | Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling |
title_full_unstemmed | Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling |
title_short | Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling |
title_sort | long noncoding rna afap1-as1 enhances cell proliferation and invasion in osteosarcoma through regulating mir-4695-5p/tcf4-β-catenin signaling |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072147/ https://www.ncbi.nlm.nih.gov/pubmed/29901121 http://dx.doi.org/10.3892/mmr.2018.9131 |
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