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Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling

Long noncoding RNA AFAP1-AS1 has been shown to promote tumor progression in several human cancer types, such as thyroid cancer, tongue squamous cell carcinoma and lung cancer. However, the role of AFAP1-AS1 in osteosarcoma (OS) has not been investigated. In the present study, the expression of AFAP1...

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Autores principales: Li, Rongrui, Liu, Shichen, Li, Yao, Tang, Qingxi, Xie, Yunchuan, Zhai, Raosheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072147/
https://www.ncbi.nlm.nih.gov/pubmed/29901121
http://dx.doi.org/10.3892/mmr.2018.9131
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author Li, Rongrui
Liu, Shichen
Li, Yao
Tang, Qingxi
Xie, Yunchuan
Zhai, Raosheng
author_facet Li, Rongrui
Liu, Shichen
Li, Yao
Tang, Qingxi
Xie, Yunchuan
Zhai, Raosheng
author_sort Li, Rongrui
collection PubMed
description Long noncoding RNA AFAP1-AS1 has been shown to promote tumor progression in several human cancer types, such as thyroid cancer, tongue squamous cell carcinoma and lung cancer. However, the role of AFAP1-AS1 in osteosarcoma (OS) has not been investigated. In the present study, the expression of AFAP1-AS1 was significantly upregulated in OS tissues and cell lines. Moreover, AFAP1-AS1 expression was negatively correlated with OS patient prognosis. Besides, AFAP1-AS1 knockdown significantly inhibited the proliferation and invasion of OS cells in vitro. Furthermore, in vivo xenograft experiments indicated that AFAP1-AS1 depletion delayed tumor growth. Regarding the underlying mechanism, AFAP1-AS1 served as a sponge to repress the level of microRNA (miR)-4695-5p, which targeted transcription factor (TCF)4, a pivot effector of Wnt/β-catenin signaling pathway. It was demonstrated that overexpression of AFAP1-AS1 inhibited the expression of miR-4695-5p, while miR-4695-5p overexpression decreased TCF4 expression and reduced activation of Wnt/β-catenin pathway. Through rescue assays, it was demonstrated that restoration of TCF4 expression reversed the effects of AFAP1-AS1 knockdown or miR-4695-5p overexpression on OS cells. Taken together, these findings demonstrated that the AFAP1-AS1/miR-4695-5p/TCF4-β-catenin axis played an important role in OS progression.
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spelling pubmed-60721472018-08-06 Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling Li, Rongrui Liu, Shichen Li, Yao Tang, Qingxi Xie, Yunchuan Zhai, Raosheng Mol Med Rep Articles Long noncoding RNA AFAP1-AS1 has been shown to promote tumor progression in several human cancer types, such as thyroid cancer, tongue squamous cell carcinoma and lung cancer. However, the role of AFAP1-AS1 in osteosarcoma (OS) has not been investigated. In the present study, the expression of AFAP1-AS1 was significantly upregulated in OS tissues and cell lines. Moreover, AFAP1-AS1 expression was negatively correlated with OS patient prognosis. Besides, AFAP1-AS1 knockdown significantly inhibited the proliferation and invasion of OS cells in vitro. Furthermore, in vivo xenograft experiments indicated that AFAP1-AS1 depletion delayed tumor growth. Regarding the underlying mechanism, AFAP1-AS1 served as a sponge to repress the level of microRNA (miR)-4695-5p, which targeted transcription factor (TCF)4, a pivot effector of Wnt/β-catenin signaling pathway. It was demonstrated that overexpression of AFAP1-AS1 inhibited the expression of miR-4695-5p, while miR-4695-5p overexpression decreased TCF4 expression and reduced activation of Wnt/β-catenin pathway. Through rescue assays, it was demonstrated that restoration of TCF4 expression reversed the effects of AFAP1-AS1 knockdown or miR-4695-5p overexpression on OS cells. Taken together, these findings demonstrated that the AFAP1-AS1/miR-4695-5p/TCF4-β-catenin axis played an important role in OS progression. D.A. Spandidos 2018-08 2018-06-05 /pmc/articles/PMC6072147/ /pubmed/29901121 http://dx.doi.org/10.3892/mmr.2018.9131 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Rongrui
Liu, Shichen
Li, Yao
Tang, Qingxi
Xie, Yunchuan
Zhai, Raosheng
Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling
title Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling
title_full Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling
title_fullStr Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling
title_full_unstemmed Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling
title_short Long noncoding RNA AFAP1-AS1 enhances cell proliferation and invasion in osteosarcoma through regulating miR-4695-5p/TCF4-β-catenin signaling
title_sort long noncoding rna afap1-as1 enhances cell proliferation and invasion in osteosarcoma through regulating mir-4695-5p/tcf4-β-catenin signaling
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072147/
https://www.ncbi.nlm.nih.gov/pubmed/29901121
http://dx.doi.org/10.3892/mmr.2018.9131
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