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Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction

Physical exercise is beneficial for the functional recovery of neurons after stroke. It has been suggested that exercise regulates proliferation and differentiation of endogenous neural stem cells (NSCs); however, the underlying molecular mechanisms are still largely unknown. In the present study, t...

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Autores principales: Liu, Wei, Wu, Wen, Lin, Guangyong, Cheng, Jian, Zeng, Yanyan, Shi, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072171/
https://www.ncbi.nlm.nih.gov/pubmed/29901080
http://dx.doi.org/10.3892/mmr.2018.9147
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author Liu, Wei
Wu, Wen
Lin, Guangyong
Cheng, Jian
Zeng, Yanyan
Shi, Yu
author_facet Liu, Wei
Wu, Wen
Lin, Guangyong
Cheng, Jian
Zeng, Yanyan
Shi, Yu
author_sort Liu, Wei
collection PubMed
description Physical exercise is beneficial for the functional recovery of neurons after stroke. It has been suggested that exercise regulates proliferation and differentiation of endogenous neural stem cells (NSCs); however, the underlying molecular mechanisms are still largely unknown. In the present study, the aim was to investigate whether physical exercise activates the extracellular signal-regulated kinase (ERK) signaling pathway to promote proliferation and differentiation of NSCs in rats with cerebral infarction, thereby improving neurological function. Following middle cerebral artery occlusion, rats underwent physical exercise and neurological behavior was analyzed at various time points. Immunofluorescence staining was performed to detect proliferation and differentiation of NSCs, and western blotting was used to analyze cyclin-dependent kinase 4 (CDK4), Cyclin D1, retinoblastoma protein (p-Rb), P-16, phosphorylated (p)-ERK1/2 and c-Fos expression. The results indicated that physical exercise promoted proliferation and differentiation of NSCs, and led to improved neural function. In addition, the expression levels of CDK4, Cyclin D1, p-Rb, p-ERK1/2 and c-Fos were upregulated, whereas the expression of P-16 was downregulated following exercise. U0126, an inhibitor of ERK signaling, reversed the beneficial effects of exercise. Therefore, it may be hypothesized that physical exercise enhances proliferation and differentiation of endogenous NSCs in the hippocampus of rats with cerebral infarction via the ERK signaling pathway.
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spelling pubmed-60721712018-08-06 Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction Liu, Wei Wu, Wen Lin, Guangyong Cheng, Jian Zeng, Yanyan Shi, Yu Mol Med Rep Articles Physical exercise is beneficial for the functional recovery of neurons after stroke. It has been suggested that exercise regulates proliferation and differentiation of endogenous neural stem cells (NSCs); however, the underlying molecular mechanisms are still largely unknown. In the present study, the aim was to investigate whether physical exercise activates the extracellular signal-regulated kinase (ERK) signaling pathway to promote proliferation and differentiation of NSCs in rats with cerebral infarction, thereby improving neurological function. Following middle cerebral artery occlusion, rats underwent physical exercise and neurological behavior was analyzed at various time points. Immunofluorescence staining was performed to detect proliferation and differentiation of NSCs, and western blotting was used to analyze cyclin-dependent kinase 4 (CDK4), Cyclin D1, retinoblastoma protein (p-Rb), P-16, phosphorylated (p)-ERK1/2 and c-Fos expression. The results indicated that physical exercise promoted proliferation and differentiation of NSCs, and led to improved neural function. In addition, the expression levels of CDK4, Cyclin D1, p-Rb, p-ERK1/2 and c-Fos were upregulated, whereas the expression of P-16 was downregulated following exercise. U0126, an inhibitor of ERK signaling, reversed the beneficial effects of exercise. Therefore, it may be hypothesized that physical exercise enhances proliferation and differentiation of endogenous NSCs in the hippocampus of rats with cerebral infarction via the ERK signaling pathway. D.A. Spandidos 2018-08 2018-06-07 /pmc/articles/PMC6072171/ /pubmed/29901080 http://dx.doi.org/10.3892/mmr.2018.9147 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Wei
Wu, Wen
Lin, Guangyong
Cheng, Jian
Zeng, Yanyan
Shi, Yu
Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction
title Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction
title_full Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction
title_fullStr Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction
title_full_unstemmed Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction
title_short Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction
title_sort physical exercise promotes proliferation and differentiation of endogenous neural stem cells via erk in rats with cerebral infarction
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072171/
https://www.ncbi.nlm.nih.gov/pubmed/29901080
http://dx.doi.org/10.3892/mmr.2018.9147
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