Cargando…
A three-lncRNA signature for prognosis prediction of acute myeloid leukemia in patients
Long non-coding RNAs (lncRNAs) are transcripts characterized by >200 nucleotides, without validated protein production. Previous studies have demonstrated that certain lncRNAs have a critical role in the initiation and development of acute myeloid leukemia (AML). In the present study, the subtype...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072220/ https://www.ncbi.nlm.nih.gov/pubmed/29901168 http://dx.doi.org/10.3892/mmr.2018.9139 |
_version_ | 1783343995204665344 |
---|---|
author | Wang, Fangce Tian, Xiaoxue Zhou, Jie Wang, Guangming Yu, Wenlei Li, Zheng Fan, Zhuoyi Zhang, Wenjun Liang, Aibin |
author_facet | Wang, Fangce Tian, Xiaoxue Zhou, Jie Wang, Guangming Yu, Wenlei Li, Zheng Fan, Zhuoyi Zhang, Wenjun Liang, Aibin |
author_sort | Wang, Fangce |
collection | PubMed |
description | Long non-coding RNAs (lncRNAs) are transcripts characterized by >200 nucleotides, without validated protein production. Previous studies have demonstrated that certain lncRNAs have a critical role in the initiation and development of acute myeloid leukemia (AML). In the present study, the subtype-specific lncRNAs in AML was identified. Following the exclusion of the subtype-specific lncRNAs, the prognostic value of lncRNAs was investigated and a three-lncRNA expression-based risk score [long intergenic non-protein coding RNA 926, family with sequence similarity 30 member A and LRRC75A antisense RNA 1 (LRRC75A-AS1)] was developed for AML patient prognosis prediction by analyzing the RNA-seq data of AML patients from Therapeutically Available Research to Generate Effective Treatments (TARGET) and The Cancer Genome Atlas (TCGA) projects. In the training set obtained from TARGET, patients were divided into poor and favorable prognosis groups by the median risk score. The prognostic effectiveness of this lncRNA risk score was confirmed in the validation set obtained from TCGA by the same cut-off. Furthermore, the lncRNA risk score was identified as an independent prognostic factor in the multivariate analysis. As further verification of the independent prognostic power of the lncRNA risk score, stratified analysis was performed by a cytogenetics risk group and revealed a consistent result. The prognostic predictive ability of the risk score was compared with the cytogenetics risk group by time-dependent receiver operating characteristic curves analysis. It was revealed that the combination of the lncRNA risk score and cytogenetics risk group provided a higher prognostic value than a single prognostic factor. The present study also performed co-expression analysis to predict the potential regulatory mechanisms of these lncRNAs in a cis/trans/competing endogenous RNA manner. The results suggested that LRRC75A-AS1 was highly associated with the target genes of transcription factors tumor protein 53 and ETS variant 6. Overall, these results highlighted the use of the three-lncRNA expression-based risk score as a potential molecular biomarker to predict the prognosis in AML patients. |
format | Online Article Text |
id | pubmed-6072220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-60722202018-08-06 A three-lncRNA signature for prognosis prediction of acute myeloid leukemia in patients Wang, Fangce Tian, Xiaoxue Zhou, Jie Wang, Guangming Yu, Wenlei Li, Zheng Fan, Zhuoyi Zhang, Wenjun Liang, Aibin Mol Med Rep Articles Long non-coding RNAs (lncRNAs) are transcripts characterized by >200 nucleotides, without validated protein production. Previous studies have demonstrated that certain lncRNAs have a critical role in the initiation and development of acute myeloid leukemia (AML). In the present study, the subtype-specific lncRNAs in AML was identified. Following the exclusion of the subtype-specific lncRNAs, the prognostic value of lncRNAs was investigated and a three-lncRNA expression-based risk score [long intergenic non-protein coding RNA 926, family with sequence similarity 30 member A and LRRC75A antisense RNA 1 (LRRC75A-AS1)] was developed for AML patient prognosis prediction by analyzing the RNA-seq data of AML patients from Therapeutically Available Research to Generate Effective Treatments (TARGET) and The Cancer Genome Atlas (TCGA) projects. In the training set obtained from TARGET, patients were divided into poor and favorable prognosis groups by the median risk score. The prognostic effectiveness of this lncRNA risk score was confirmed in the validation set obtained from TCGA by the same cut-off. Furthermore, the lncRNA risk score was identified as an independent prognostic factor in the multivariate analysis. As further verification of the independent prognostic power of the lncRNA risk score, stratified analysis was performed by a cytogenetics risk group and revealed a consistent result. The prognostic predictive ability of the risk score was compared with the cytogenetics risk group by time-dependent receiver operating characteristic curves analysis. It was revealed that the combination of the lncRNA risk score and cytogenetics risk group provided a higher prognostic value than a single prognostic factor. The present study also performed co-expression analysis to predict the potential regulatory mechanisms of these lncRNAs in a cis/trans/competing endogenous RNA manner. The results suggested that LRRC75A-AS1 was highly associated with the target genes of transcription factors tumor protein 53 and ETS variant 6. Overall, these results highlighted the use of the three-lncRNA expression-based risk score as a potential molecular biomarker to predict the prognosis in AML patients. D.A. Spandidos 2018-08 2018-06-06 /pmc/articles/PMC6072220/ /pubmed/29901168 http://dx.doi.org/10.3892/mmr.2018.9139 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wang, Fangce Tian, Xiaoxue Zhou, Jie Wang, Guangming Yu, Wenlei Li, Zheng Fan, Zhuoyi Zhang, Wenjun Liang, Aibin A three-lncRNA signature for prognosis prediction of acute myeloid leukemia in patients |
title | A three-lncRNA signature for prognosis prediction of acute myeloid leukemia in patients |
title_full | A three-lncRNA signature for prognosis prediction of acute myeloid leukemia in patients |
title_fullStr | A three-lncRNA signature for prognosis prediction of acute myeloid leukemia in patients |
title_full_unstemmed | A three-lncRNA signature for prognosis prediction of acute myeloid leukemia in patients |
title_short | A three-lncRNA signature for prognosis prediction of acute myeloid leukemia in patients |
title_sort | three-lncrna signature for prognosis prediction of acute myeloid leukemia in patients |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072220/ https://www.ncbi.nlm.nih.gov/pubmed/29901168 http://dx.doi.org/10.3892/mmr.2018.9139 |
work_keys_str_mv | AT wangfangce athreelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT tianxiaoxue athreelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT zhoujie athreelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT wangguangming athreelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT yuwenlei athreelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT lizheng athreelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT fanzhuoyi athreelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT zhangwenjun athreelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT liangaibin athreelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT wangfangce threelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT tianxiaoxue threelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT zhoujie threelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT wangguangming threelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT yuwenlei threelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT lizheng threelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT fanzhuoyi threelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT zhangwenjun threelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients AT liangaibin threelncrnasignatureforprognosispredictionofacutemyeloidleukemiainpatients |