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Interaction with adipocytes induces lung adenocarcinoma A549 cell migration and tumor growth

Adipocytes have been demonstrated to promote the progression of various tumors through modulation of cancer cell metabolism. However, their role in lung cancer progression remains undetermined. In the present study, adipocytes and lung adenocarcinoma A549 cells were cultured in a Transwell co-cultur...

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Autores principales: Li, Fan-Fan, Zhang, Hang, Li, Jing-Jing, Cao, Ya-Nan, Dong, Xiang, Gao, Cong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072224/
https://www.ncbi.nlm.nih.gov/pubmed/29956800
http://dx.doi.org/10.3892/mmr.2018.9226
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author Li, Fan-Fan
Zhang, Hang
Li, Jing-Jing
Cao, Ya-Nan
Dong, Xiang
Gao, Cong
author_facet Li, Fan-Fan
Zhang, Hang
Li, Jing-Jing
Cao, Ya-Nan
Dong, Xiang
Gao, Cong
author_sort Li, Fan-Fan
collection PubMed
description Adipocytes have been demonstrated to promote the progression of various tumors through modulation of cancer cell metabolism. However, their role in lung cancer progression remains undetermined. In the present study, adipocytes and lung adenocarcinoma A549 cells were cultured in a Transwell co-culture system. Cancer cells were additionally cultured in conditioned medium, obtained from adipocytes or co-cultured cells. A MTT and colony formation assay were performed to assess A549 cell proliferation. The expression of epithelial-mesenchymal transition protein markers E-cadherin and vimentin were measured by western blotting. A549 cell migration and invasion was determined with wound healing, Transwell and Matrigel assays. Oil Red-O staining was used to evaluate intracellular lipid content. Colorimetric assays were utilized to detect free fatty acid, glucose uptake, lactate production and triglyceride content in cells. The results revealed a reciprocal interaction between adipocytes and A549 cells, which significantly enhanced A549 cell proliferation and metastasis; whereas, the expression of E-cadherin was decreased and vimentin was increased in A549 cells. Additionally, A549 cells exhibited metabolic reprogramming in vitro following co-culture with adipocytes. It was demonstrated that lipid droplets accumulation, glucose consumption and lactate production increased in tumor cells exposed to adipocytes. Furthermore, adipocytes co-cultured with A549 cells exhibited a decrease in the number and size of lipid droplets, a decrease in the intracellular triglyceride content and a significant increase in the release of free fatty acids. These findings highlighted the crucial role of adipocytes in the modulation of lung adenocarcinoma A549 cell metabolism and suggested the involvement of adipocytes in lung cancer progression.
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spelling pubmed-60722242018-08-06 Interaction with adipocytes induces lung adenocarcinoma A549 cell migration and tumor growth Li, Fan-Fan Zhang, Hang Li, Jing-Jing Cao, Ya-Nan Dong, Xiang Gao, Cong Mol Med Rep Articles Adipocytes have been demonstrated to promote the progression of various tumors through modulation of cancer cell metabolism. However, their role in lung cancer progression remains undetermined. In the present study, adipocytes and lung adenocarcinoma A549 cells were cultured in a Transwell co-culture system. Cancer cells were additionally cultured in conditioned medium, obtained from adipocytes or co-cultured cells. A MTT and colony formation assay were performed to assess A549 cell proliferation. The expression of epithelial-mesenchymal transition protein markers E-cadherin and vimentin were measured by western blotting. A549 cell migration and invasion was determined with wound healing, Transwell and Matrigel assays. Oil Red-O staining was used to evaluate intracellular lipid content. Colorimetric assays were utilized to detect free fatty acid, glucose uptake, lactate production and triglyceride content in cells. The results revealed a reciprocal interaction between adipocytes and A549 cells, which significantly enhanced A549 cell proliferation and metastasis; whereas, the expression of E-cadherin was decreased and vimentin was increased in A549 cells. Additionally, A549 cells exhibited metabolic reprogramming in vitro following co-culture with adipocytes. It was demonstrated that lipid droplets accumulation, glucose consumption and lactate production increased in tumor cells exposed to adipocytes. Furthermore, adipocytes co-cultured with A549 cells exhibited a decrease in the number and size of lipid droplets, a decrease in the intracellular triglyceride content and a significant increase in the release of free fatty acids. These findings highlighted the crucial role of adipocytes in the modulation of lung adenocarcinoma A549 cell metabolism and suggested the involvement of adipocytes in lung cancer progression. D.A. Spandidos 2018-08 2018-06-26 /pmc/articles/PMC6072224/ /pubmed/29956800 http://dx.doi.org/10.3892/mmr.2018.9226 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Fan-Fan
Zhang, Hang
Li, Jing-Jing
Cao, Ya-Nan
Dong, Xiang
Gao, Cong
Interaction with adipocytes induces lung adenocarcinoma A549 cell migration and tumor growth
title Interaction with adipocytes induces lung adenocarcinoma A549 cell migration and tumor growth
title_full Interaction with adipocytes induces lung adenocarcinoma A549 cell migration and tumor growth
title_fullStr Interaction with adipocytes induces lung adenocarcinoma A549 cell migration and tumor growth
title_full_unstemmed Interaction with adipocytes induces lung adenocarcinoma A549 cell migration and tumor growth
title_short Interaction with adipocytes induces lung adenocarcinoma A549 cell migration and tumor growth
title_sort interaction with adipocytes induces lung adenocarcinoma a549 cell migration and tumor growth
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072224/
https://www.ncbi.nlm.nih.gov/pubmed/29956800
http://dx.doi.org/10.3892/mmr.2018.9226
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