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Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma-bearing mouse model

Tetrandrine (TET), a traditional Chinese clinical agent, has been used for the treatment of many diseases, including cancers. The purpose of the present study was to investigate the combined effects of TET and ionizing radiation (IR) on murine CT26 colorectal adenocarcinoma cells in vitro and in viv...

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Autores principales: Lin, Wei-Chan, Wang, Wei-Hsun, Lin, Yi-Hsien, Leu, Jyh-Der, Cheng, Shan-Yun, Chen, Yu-Jen, Hwang, Jeng-Jong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072404/
https://www.ncbi.nlm.nih.gov/pubmed/30015952
http://dx.doi.org/10.3892/or.2018.6568
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author Lin, Wei-Chan
Wang, Wei-Hsun
Lin, Yi-Hsien
Leu, Jyh-Der
Cheng, Shan-Yun
Chen, Yu-Jen
Hwang, Jeng-Jong
author_facet Lin, Wei-Chan
Wang, Wei-Hsun
Lin, Yi-Hsien
Leu, Jyh-Der
Cheng, Shan-Yun
Chen, Yu-Jen
Hwang, Jeng-Jong
author_sort Lin, Wei-Chan
collection PubMed
description Tetrandrine (TET), a traditional Chinese clinical agent, has been used for the treatment of many diseases, including cancers. The purpose of the present study was to investigate the combined effects of TET and ionizing radiation (IR) on murine CT26 colorectal adenocarcinoma cells in vitro and in vivo. A CT26 cell line transfected with dual HSV-1 thymidine kinase and firefly luciferase (luc) reporter genes was used. The half-maximal inhibitory concentration (IC(50)) of TET in CT26/tk-luc cells was ~10 µM. An additive effect was observed after combination of both agents based on a colony formation assay. Apoptosis and cleaved caspase-3 levels were increased significantly in cells after combination treatment, as shown by flow cytometric analysis, DNA fragmentation and western blotting. However, tumor growth inhibition and therapeutic efficacy of TET combined with IR in vivo were identified to be synergistic, as monitored by tumor growth delay time, measured with a digital caliper. A significant inhibition of tumor growth was identified in the combination group compared with the radiation only group. Furthermore, non-invasive bioluminescent imaging (BLI) and gamma scintigraphy were also used to evaluate therapeutic efficacy. Both modalities revealed that the best tumor growth control was under combination treatment among all groups. The present study demonstrated that TET is not only beneficial for chemotherapy, but also has potential as a radiosensitizer for the treatment of cancer.
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spelling pubmed-60724042018-08-30 Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma-bearing mouse model Lin, Wei-Chan Wang, Wei-Hsun Lin, Yi-Hsien Leu, Jyh-Der Cheng, Shan-Yun Chen, Yu-Jen Hwang, Jeng-Jong Oncol Rep Articles Tetrandrine (TET), a traditional Chinese clinical agent, has been used for the treatment of many diseases, including cancers. The purpose of the present study was to investigate the combined effects of TET and ionizing radiation (IR) on murine CT26 colorectal adenocarcinoma cells in vitro and in vivo. A CT26 cell line transfected with dual HSV-1 thymidine kinase and firefly luciferase (luc) reporter genes was used. The half-maximal inhibitory concentration (IC(50)) of TET in CT26/tk-luc cells was ~10 µM. An additive effect was observed after combination of both agents based on a colony formation assay. Apoptosis and cleaved caspase-3 levels were increased significantly in cells after combination treatment, as shown by flow cytometric analysis, DNA fragmentation and western blotting. However, tumor growth inhibition and therapeutic efficacy of TET combined with IR in vivo were identified to be synergistic, as monitored by tumor growth delay time, measured with a digital caliper. A significant inhibition of tumor growth was identified in the combination group compared with the radiation only group. Furthermore, non-invasive bioluminescent imaging (BLI) and gamma scintigraphy were also used to evaluate therapeutic efficacy. Both modalities revealed that the best tumor growth control was under combination treatment among all groups. The present study demonstrated that TET is not only beneficial for chemotherapy, but also has potential as a radiosensitizer for the treatment of cancer. D.A. Spandidos 2018-09 2018-07-12 /pmc/articles/PMC6072404/ /pubmed/30015952 http://dx.doi.org/10.3892/or.2018.6568 Text en Copyright: © Lin et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Lin, Wei-Chan
Wang, Wei-Hsun
Lin, Yi-Hsien
Leu, Jyh-Der
Cheng, Shan-Yun
Chen, Yu-Jen
Hwang, Jeng-Jong
Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma-bearing mouse model
title Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma-bearing mouse model
title_full Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma-bearing mouse model
title_fullStr Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma-bearing mouse model
title_full_unstemmed Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma-bearing mouse model
title_short Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma-bearing mouse model
title_sort synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma-bearing mouse model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072404/
https://www.ncbi.nlm.nih.gov/pubmed/30015952
http://dx.doi.org/10.3892/or.2018.6568
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