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Resistance Response of Chilli (Capsicum annuum L.) F(1) to Fusarium oxysporum Involves Expression of the CaChi2 Gene

Cross-breeding is a method of producing progeny with better resistance to pathogens. Resistance to pathogens usually involves pathogenesis-related (PR) proteins. Class II chitinase is an example of a defensive PR protein in plants. The class II chitinase in chilli is coded by the CaChi2 gene. In thi...

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Autores principales: Ferniah, Rejeki Siti, Kasiamdari, Rina Sri, Priyatmojo, Achmadi, Daryono, Budi Setiadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Penerbit Universiti Sains Malaysia 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072719/
https://www.ncbi.nlm.nih.gov/pubmed/30112139
http://dx.doi.org/10.21315/tlsr2018.29.2.3
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author Ferniah, Rejeki Siti
Kasiamdari, Rina Sri
Priyatmojo, Achmadi
Daryono, Budi Setiadi
author_facet Ferniah, Rejeki Siti
Kasiamdari, Rina Sri
Priyatmojo, Achmadi
Daryono, Budi Setiadi
author_sort Ferniah, Rejeki Siti
collection PubMed
description Cross-breeding is a method of producing progeny with better resistance to pathogens. Resistance to pathogens usually involves pathogenesis-related (PR) proteins. Class II chitinase is an example of a defensive PR protein in plants. The class II chitinase in chilli is coded by the CaChi2 gene. In this study, we crossed susceptible with resistant chilli cultivars, analysed the F(1) resistance response against pathogenic F. oxysporum, and analysed the level of CaChi2 gene expression in the F(1). Data were collected using disease severity index (DSI) determination and gene expression analysis by qRT-PCR (quantitative Reverse Transcriptase Polymerase Chain Reaction). Results showed that the DSI of F(1) was not significantly different from the resistant ancestor. The relative CaChi2 expression level of F(1) was higher than the susceptible ancestor but not significantly different from the resistant ancestor. We concluded that the F(1) can be categorised as resistant to F. oxysporum, and the CaChi2 gene is involved in the molecular defense response.
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spelling pubmed-60727192018-08-15 Resistance Response of Chilli (Capsicum annuum L.) F(1) to Fusarium oxysporum Involves Expression of the CaChi2 Gene Ferniah, Rejeki Siti Kasiamdari, Rina Sri Priyatmojo, Achmadi Daryono, Budi Setiadi Trop Life Sci Res Articles Cross-breeding is a method of producing progeny with better resistance to pathogens. Resistance to pathogens usually involves pathogenesis-related (PR) proteins. Class II chitinase is an example of a defensive PR protein in plants. The class II chitinase in chilli is coded by the CaChi2 gene. In this study, we crossed susceptible with resistant chilli cultivars, analysed the F(1) resistance response against pathogenic F. oxysporum, and analysed the level of CaChi2 gene expression in the F(1). Data were collected using disease severity index (DSI) determination and gene expression analysis by qRT-PCR (quantitative Reverse Transcriptase Polymerase Chain Reaction). Results showed that the DSI of F(1) was not significantly different from the resistant ancestor. The relative CaChi2 expression level of F(1) was higher than the susceptible ancestor but not significantly different from the resistant ancestor. We concluded that the F(1) can be categorised as resistant to F. oxysporum, and the CaChi2 gene is involved in the molecular defense response. Penerbit Universiti Sains Malaysia 2018-07 2018-07-06 /pmc/articles/PMC6072719/ /pubmed/30112139 http://dx.doi.org/10.21315/tlsr2018.29.2.3 Text en © Penerbit Universiti Sains Malaysia, 2018 This work is licensed under the terms of the Creative Commons Attribution (CC BY) (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Articles
Ferniah, Rejeki Siti
Kasiamdari, Rina Sri
Priyatmojo, Achmadi
Daryono, Budi Setiadi
Resistance Response of Chilli (Capsicum annuum L.) F(1) to Fusarium oxysporum Involves Expression of the CaChi2 Gene
title Resistance Response of Chilli (Capsicum annuum L.) F(1) to Fusarium oxysporum Involves Expression of the CaChi2 Gene
title_full Resistance Response of Chilli (Capsicum annuum L.) F(1) to Fusarium oxysporum Involves Expression of the CaChi2 Gene
title_fullStr Resistance Response of Chilli (Capsicum annuum L.) F(1) to Fusarium oxysporum Involves Expression of the CaChi2 Gene
title_full_unstemmed Resistance Response of Chilli (Capsicum annuum L.) F(1) to Fusarium oxysporum Involves Expression of the CaChi2 Gene
title_short Resistance Response of Chilli (Capsicum annuum L.) F(1) to Fusarium oxysporum Involves Expression of the CaChi2 Gene
title_sort resistance response of chilli (capsicum annuum l.) f(1) to fusarium oxysporum involves expression of the cachi2 gene
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072719/
https://www.ncbi.nlm.nih.gov/pubmed/30112139
http://dx.doi.org/10.21315/tlsr2018.29.2.3
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