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Deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation
The hippocampus is associated with memory and navigation, and the rodent hippocampus provides a useful model system for studying neurophysiology such as neural plasticity. Vascular changes at this site are closely related to brain diseases, such as Alzheimer’s disease, dementia, and epilepsy. Vascul...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072748/ https://www.ncbi.nlm.nih.gov/pubmed/30072791 http://dx.doi.org/10.1038/s41598-018-29975-6 |
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author | Park, Kwan Seob Shin, Jun Geun Qureshi, Muhammad Mohsin Chung, Euiheon Eom, Tae Joong |
author_facet | Park, Kwan Seob Shin, Jun Geun Qureshi, Muhammad Mohsin Chung, Euiheon Eom, Tae Joong |
author_sort | Park, Kwan Seob |
collection | PubMed |
description | The hippocampus is associated with memory and navigation, and the rodent hippocampus provides a useful model system for studying neurophysiology such as neural plasticity. Vascular changes at this site are closely related to brain diseases, such as Alzheimer’s disease, dementia, and epilepsy. Vascular imaging around the hippocampus in mice may help to further elucidate the mechanisms underlying these diseases. Optical coherence tomography angiography (OCTA) is an emerging technology that can provide label-free blood flow information. As the hippocampus is a deep structure in the mouse brain, direct in vivo visualisation of the vascular network using OCTA and other microscopic imaging modalities has been challenging. Imaging of blood vessels in the hippocampus has been performed using multiphoton microscopy; however, labelling with fluorescence probes is necessary when using this technique. Here, we report the use of label-free and noninvasive microvascular imaging in the hippocampal formation of mice using a 1.7-μm swept-source OCT system. The imaging results demonstrate that the proposed system can visualise blood flow at different locations of the hippocampus corresponding with deep brain areas. |
format | Online Article Text |
id | pubmed-6072748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60727482018-08-06 Deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation Park, Kwan Seob Shin, Jun Geun Qureshi, Muhammad Mohsin Chung, Euiheon Eom, Tae Joong Sci Rep Article The hippocampus is associated with memory and navigation, and the rodent hippocampus provides a useful model system for studying neurophysiology such as neural plasticity. Vascular changes at this site are closely related to brain diseases, such as Alzheimer’s disease, dementia, and epilepsy. Vascular imaging around the hippocampus in mice may help to further elucidate the mechanisms underlying these diseases. Optical coherence tomography angiography (OCTA) is an emerging technology that can provide label-free blood flow information. As the hippocampus is a deep structure in the mouse brain, direct in vivo visualisation of the vascular network using OCTA and other microscopic imaging modalities has been challenging. Imaging of blood vessels in the hippocampus has been performed using multiphoton microscopy; however, labelling with fluorescence probes is necessary when using this technique. Here, we report the use of label-free and noninvasive microvascular imaging in the hippocampal formation of mice using a 1.7-μm swept-source OCT system. The imaging results demonstrate that the proposed system can visualise blood flow at different locations of the hippocampus corresponding with deep brain areas. Nature Publishing Group UK 2018-08-02 /pmc/articles/PMC6072748/ /pubmed/30072791 http://dx.doi.org/10.1038/s41598-018-29975-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Park, Kwan Seob Shin, Jun Geun Qureshi, Muhammad Mohsin Chung, Euiheon Eom, Tae Joong Deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation |
title | Deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation |
title_full | Deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation |
title_fullStr | Deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation |
title_full_unstemmed | Deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation |
title_short | Deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation |
title_sort | deep brain optical coherence tomography angiography in mice: in vivo, noninvasive imaging of hippocampal formation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072748/ https://www.ncbi.nlm.nih.gov/pubmed/30072791 http://dx.doi.org/10.1038/s41598-018-29975-6 |
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